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Evaluation of Cardiomyocyte Viability after Cardiac Tissue Incubated in Special Culture System

Published online by Cambridge University Press:  02 July 2020

C. Wei  and
J Papadimitriou
C. Wei
Affiliation:
Cardiovascular Molecular Research, Departments of Surgery and Pathology, University of Maryland School of Medicine, Baltimore, MD, 21201
J Papadimitriou
Affiliation:
Cardiovascular Molecular Research, Departments of Surgery and Pathology, University of Maryland School of Medicine, Baltimore, MD, 21201
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Extract

Cardiac myocytes maintained in cell culture develop hypertrophy both in response to mechanical loading as well as to receptor-mediated signaling mechanisms. However, it has been shown that the hypertrophic response to these stimuli may be modulated through effects of intercellular contact achieved by maintaining cells at different plating densities. We recently developed the new special tissue culture system to culture minced cardiac tissue and to evaluate the apoptotic effect of different agent in cardiomyocytes. After 24 hours culture period, different culture conditions and factors may damage the cardiomyocyte. Therefore, we performed different methods to evaluate the viability of cardiomyocyte in cardiac tissue with 24 hours culture period in our special culture system.

Human cardiac atrial tissue was obtained from open-heart surgery (n=6). After cardiac tissue excision, the samples were immediately placed in oxygenated, nominally Ca2+-free Tyrode solution for transport to the laboratory. The time between excision and the beginning of laboratory processing was five to fifteen minutes.

Type
Pathology
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 620 - 621
Copyright
Copyright © Microscopy Society of America

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References

References:

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4. This research was supported in part by grants from the NIH (HL03174 & HL61299, C. Wei), AHAMD, NKF and University of Maryland School of Medicine.Google Scholar