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Environmental Scanning Electron Microscopy Technique to Identify Asbestos Phases Inside Ferruginous Bodies

Published online by Cambridge University Press:  26 February 2013

Alessandro Croce ,
Maya Musa ,
Mario Allegrina ,
Paolo Trivero  and
Caterina Rinaudo
Alessandro Croce
Affiliation:
Department of Science and Technological Innovation, Università del Piemonte Orientale “Amedeo Avogadro,”Viale Teresa Michel 11, 15121 Alessandria, Italy
Maya Musa
Affiliation:
Department of Science and Technological Innovation, Università del Piemonte Orientale “Amedeo Avogadro,”Viale Teresa Michel 11, 15121 Alessandria, Italy
Mario Allegrina
Affiliation:
Department of Science and Technological Innovation, Università del Piemonte Orientale “Amedeo Avogadro,”Viale Teresa Michel 11, 15121 Alessandria, Italy
Paolo Trivero
Affiliation:
Department of Science and Technological Innovation, Università del Piemonte Orientale “Amedeo Avogadro,”Viale Teresa Michel 11, 15121 Alessandria, Italy
Caterina Rinaudo*
Affiliation:
Department of Science and Technological Innovation, Università del Piemonte Orientale “Amedeo Avogadro,”Viale Teresa Michel 11, 15121 Alessandria, Italy
*
*Corresponding author. E-mail: caterina.rinaudo@mfn.unipmn.it
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Abstract

Ferruginous bodies observed in lungs of patients affected by mesothelioma, asbestosis, and pulmonary carcinoma are important to relate the illness to exposure, environmental or occupational, to asbestos. Identification of the inorganic phase constituting the core of the ferruginous bodies, formed around asbestos but also around phases different from asbestos, is essential for legal purposes. Environmental scanning electron microscopy/energy dispersive spectroscopy was used to identify the fibrous mineral phase in the core of ferruginous bodies observed directly in thin sections of tissue, without digestion of the biological matrix. Spectra were taken with sequential analyses along a line crossing the core of the ferruginous bodies. By comparing the spectra taken near to and far from the core, the chemical elements that make up the core could be identified.

Keywords

ESEM/EDSasbestosferruginous bodiesmalignant mesothelioma
Type
Biological Applications: Short Communications
Information
Microscopy and Microanalysis , Volume 19 , Issue 2 , April 2013 , pp. 420 - 424
Copyright
Copyright © Microscopy Society of America 2013

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