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Electron Microscopy Findings in N-Methyl-N-Nitrosourea-Induced Mammary Tumors

Published online by Cambridge University Press:  22 September 2016

Ana I. Faustino-Rocha ,
Ana M. Calado ,
Adelina Gama ,
Rita Ferreira ,
Mário Ginja  and
Paula A. Oliveira
Ana I. Faustino-Rocha*
Affiliation:
Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801, Vila Real, Portugal Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), UTAD, 5001-801, Vila Real, Portugal Animal and Veterinary Research Center (CECAV), UTAD, 5001-801, Vila Real, Portugal Organic Chemistry, Natural Products and Foodstuffs (QOPNA), Department of Chemistry, Mass Spectrometry Center, University of Aveiro, 3810-193, Aveiro, Portugal
Ana M. Calado
Affiliation:
Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801, Vila Real, Portugal
Adelina Gama
Affiliation:
Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801, Vila Real, Portugal Animal and Veterinary Research Center (CECAV), UTAD, 5001-801, Vila Real, Portugal
Rita Ferreira
Affiliation:
Organic Chemistry, Natural Products and Foodstuffs (QOPNA), Department of Chemistry, Mass Spectrometry Center, University of Aveiro, 3810-193, Aveiro, Portugal
Mário Ginja
Affiliation:
Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801, Vila Real, Portugal Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), UTAD, 5001-801, Vila Real, Portugal
Paula A. Oliveira
Affiliation:
Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801, Vila Real, Portugal Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), UTAD, 5001-801, Vila Real, Portugal
*
*Corresponding author. anafaustino.faustino@sapo.pt
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Abstract

Although the rat model of mammary tumors chemically induced by N-methyl-N-nitrosourea (MNU) has been frequently used by several research teams, there is a lack of ultrastructural studies in this field. The main aim of this work was to perform an ultrastructural characterization of MNU-induced mammary tumors in female rats. Some alterations previously reported in human mammary tumors, such as nucleus size and shape, accumulation of heterochromatin in the perinuclear region, and interdigitating cytoplasmic processes between cancer cells were also observed in MNU-induced mammary tumors. Although a low number of samples were analyzed by transmission electron microscopy in the present study, we consider that it may contribute to a better understanding of MNU-induced mammary carcinogenesis in a rat model. The ultrastructural characteristics of the two most frequently diagnosed mammary carcinomas described in the present work can be useful to differentiate them from other histological patterns. In addition, the loss of cytoplasm in neoplastic cells and formation of vacuoles were described.

Keywords

N-methyl-N-nitrosourea-induced mammary tumorratSprague-Dawleytransmission electron microscopyultrastructure
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 22 , Issue 5 , October 2016 , pp. 1056 - 1061
Copyright
© Microscopy Society of America 2016 

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References

Benbow, U., Schoenermark, M.P., Orndorff, K.A., Givan, A.L. & Brinckerhoff, C.E. (1999). Human breast cancer cells activate procollagenase-1 and invade type I collagen: Invasion is inhibited by all-trans retinoic acid. Clin Exp Metastasis 17(3), 231–238.Google Scholar
Calado, A.M., Oliveira, E., Colaco, A. & Sousa, M. (2011). Ultrastructural and cytochemical characterization of follicular cell types in bovine (Bos taurus) cumulus-oocyte complexes aspirated from small and medium antral follicles during the estrus cycle. Anim Reprod Sci 123(1–2), 23–31.Google Scholar
Faustino-Rocha, A.I., Ferreira, R., Oliveira, P.A., Gama, A. & Ginja, M. (2015 a). N-Methyl-N-nitrosourea as a mammary carcinogenic agent. Tumour Biol 36(12), 9095–9117.Google Scholar
Faustino-Rocha, A.I., Gama, A., Oliveira, P.A., Neuparth, M., Ferreira, R. & Ginja, M. (2016). Effects of lifelong exercise training on mammary tumorigenesis induced by MNU in female Sprague-Dawley rats. Clin Exp Med. doi:10.1007/s10238-016-0419-0 [Epub ahead of print].Google ScholarPubMed
Faustino-Rocha, A.I., Gama, A., Oliveira, P.A., Vala, H., Ferreira, R. & Ginja, M. (2015 b). Effects of histamine on the development of MNU-induced mammary tumours. Virchows Archiv 467, S51–S51.Google Scholar
Faustino-Rocha, A.I., Gama, A., Pires, M.J., Oliveira, P.A. & Ginja, M. (2014 a). Effects of a mast cell stabilizer on the vascularization of mammary tumors: Ultrasonographic evaluation. Anticancer Res 34(10), 5901–5901.Google Scholar
Faustino-Rocha, A.I., Oliveira, P.A., Duarte, J.A., Ferreira, R. & Ginja, M. (2013 a). Ultrasonographic evaluation of gastrocnemius muscle in a rat model of N-Methyl-N-nitrosourea-induced mammary tumor. In Vivo 27(6), 803–807.Google Scholar
Faustino-Rocha, A.I., Pinto, C., Gama, A. & Oliveira, P.A. (2014 b). Effects of ketotifen on mammary tumors volume and weight. Anticancer Res 34(10), 5902–5902.Google Scholar
Faustino-Rocha, A.I., Silva, A., Gabriel, J., Teixeira-Guedes, C.I., Lopes, C., da Costa, R.G., Gama, A., Ferreira, R., Oliveira, P.A. & Ginja, M. (2013 b). Ultrasonographic, thermographic and histologic evaluation of MNU-induced mammary tumors in female Sprague-Dawley rats. Biomed Pharmacother 67(8), 771–776.Google Scholar
Forbes, D., Blom, H., Kostomitsopulos, N., Moore, G. & Perretta, G. (Eds) (2007). Euroguide: On the Accommodation and Care of Animals Used for Experimental and Other Scientific Purposes. London: Federation of European Laboratory Animal Science Associations.Google Scholar
Ghadially, F. & Parry, E. (1966). Ultrastructure of a human hepatocellular carcinoma and surrounding non-neoplastic liver. Cancer 19(12), 1989–2004.Google Scholar
Ghadially, F.N. (1985). Differences between normal and neoplastic cells. In Diagnostic Electron Microscopy of Tumors, Ghadially, F.N. (ed.), pp. 25–62. London: Butterworths.Google Scholar
Hyttel, P., Xu, K., Smith, S. & Greve, T. (1986). Ultrastructure of in-vitro oocyte maturation in cattle. J Reprod Fertil 78(2), 615–625.CrossRefGoogle ScholarPubMed
Jaafar, H., Sharif, S.E.T. & Das Murtey, M. (2012). Distinctive features of advancing breast cancer cells and interactions with surrounding stroma observed under the scanning electron microscope. Asian Pac J Cancer Prev 13(4), 1305–1310.CrossRefGoogle ScholarPubMed
Kataoka, S. & Tsuruo, T. (1996). Physician education: Apoptosis. Oncologist 1, 399–401.Google Scholar
Kaul-Ghanekar, R., Singh, S., Mamgain, H., Jalota-Badhwar, A., Paknikar, K.M. & Chattopadhyay, S. (2009). Tumor suppressor protein SMAR1 modulates the roughness of cell surface: Combined AFM and SEM study. BMC Cancer 9, 350.Google Scholar
Kramer, R.H., Bensch, K.G. & Wong, J. (1986). Invasion of reconstituted basement-membrane matrix by metastatic human-tumor cells. Cancer Res 46(4), 1980–1989.Google Scholar
Luparello, C., Sheterline, P., Pucciminafra, I. & Minafra, S. (1991). A comparison of spreading and motility behavior of 8701-Bc breast-carcinoma cells on Type-I, I-Trimer and Type-V collagen substrata—evidence for a permissive effect of Type I-Trimer collagen on cell locomotion. J Cell Sci 100, 179–185.Google Scholar
Ribatti, D. & Crivellato, E. (2011). Mast cells and tumors. In Mast cells and tumors: from biology to clinic, Ribatti, D. & Crivellato, E. (Eds.), pp. 83–88. New York: Springer.Google Scholar
Russo, J., Bradley, R.H., Mcgrath, C. & Russo, I.H. (1977). Scanning and transmission electron-microscopy study of a human breast carcinoma cell line (MCF-7) cultured in collagen-coated cellulose sponge. Cancer Res 37(7), 2004–2014.Google Scholar
Russo, J. & Russo, I.H. (2000). Atlas and histologic classification of tumors of the rat mammary gland. J Mammary Gland Biol Neoplasia 5(2), 187–200.Google Scholar
Scott, M.A., Lagios, M.D., Axelsson, K., Rogers, L.W., Anderson, T.J. & Page, D.L. (1997). Ductal carcinoma in situ of the breast: Reproducibility of histological subtype analysis. Hum Pathol 28(8), 967–973.CrossRefGoogle ScholarPubMed
Sloane, J. (Ed.) (1985). Biopsy Pathology of the Breast, pp. 39–40. London: Chapman & Hall.Google Scholar
Soares-Maia, R., Faustino-Rocha, A.I., Teixeira-Guedes, C.I., Pinho-Oliveira, J., Talhada, D., Rema, A., Faria, F., Ginja, M., Ferreira, R., da Costa, R.M.G., Oliveira, P.A. & Lopes, C. (2013). MNU-Induced rat mammary carcinomas: Immunohistology and estrogen receptor expression. J Environ Pathol Toxicol Oncol 32(2), 157–163.Google Scholar
Tsuchiya, S. & Li, F. (2005). Electron microscopic findings for diagnosis of breast lesions. Med Mol Morphol 38, 216–224.CrossRefGoogle ScholarPubMed
Winey, M., Meehl, J.B., O’Toole, E.T. & Giddings, T.H. (2014). Conventional transmission electron microscopy. Mol Biol Cell 25, 319–323.Google Scholar