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The Effects of Cyclosporin-A on Human Vascular Smooth Muscle Cells Apoptosis

Published online by Cambridge University Press:  02 July 2020

M. Kinjo ,
J. S. McLaughlin  and
C. Wei.
M. Kinjo
Affiliation:
Cardiovascular Molecular Research, Department of Surgery, University of Maryland School of Medicine, Baltimore, MD, 21201
J. S. McLaughlin
Affiliation:
Cardiovascular Molecular Research, Department of Surgery, University of Maryland School of Medicine, Baltimore, MD, 21201
C. Wei.
Affiliation:
Cardiovascular Molecular Research, Department of Surgery, University of Maryland School of Medicine, Baltimore, MD, 21201
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Extract

Cyclosporine-A is the one of the most effective immunosuppressant drug in organ transplantation. However, cyclosporine-A has a potent cytotoxic effect. Cellular apoptosis is being suggested may contribute to the cyclosporine A-mediated cytotoxic action. To date, regarding the effects of cyclosporine-A on apoptosis and apoptosis-related genes expression in vascular smooth muscle cells remain unclear. Therefore, the current study was designed to investigate the actions of cyclosporine-A on apoptosis and p53 expression in human vascular smooth muscle cells.

Human saphenous vein was obtained from coronary artery bypass surgery (n=5). After saphenous vein removed, the tissue was minced and incubated in the special tissue culture system for 24 hours in the absence or presence of cyclosporine-A (10-6M). To detect the DNA fragmentation, in situ terminal deoxymucleotidyl transferase dUTP nick end labeling (TUNEL) was performed. The p53 level in human vascular smooth muscle cells was determined by immunohistochemical staining (IHCS). An average of 1000 nuclei was analyzed for both TUNEL and p53 EHCS studies.

Type
Apoptosis
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 634 - 635
Copyright
Copyright © Microscopy Society of America

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References

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5. This research was supported in part by grants from the N1H (HL03174 & HL61299, C. Wei), AHAMD, NKF and University of Maryland School of Medicine.Google Scholar