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CryoET Data Collection and Subtomogram Averaging Using emClarity

Published online by Cambridge University Press:  30 July 2020

Tao Ni
Affiliation:
University of Oxford, Oxford, England, United Kingdom
Peijun Zhang
Affiliation:
University of Oxford, Oxford, England, United Kingdom
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Abstract

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Type
The Promise of Cryo-Electron Tomography
Copyright
Copyright © Microscopy Society of America 2020

References

Zhang, P. Advances in cryo-electron tomography and subtomogram averaging and classification. Curr. Opin. Struct. Biol. 58 , 249258 (2019).10.1016/j.sbi.2019.05.021CrossRefGoogle ScholarPubMed
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Hagen, W. J. H., Wan, W. & Briggs, J. A. G. Implementation of a cryo-electron tomography tilt-scheme optimized for high resolution subtomogram averaging. J. Struct. Biol. 197 , 191198 (2017).10.1016/j.jsb.2016.06.007CrossRefGoogle ScholarPubMed
Mastronarde, D. N. & Held, S. R. Automated tilt series alignment and tomographic reconstruction in IMOD. J. Struct. Biol. 197 , 102113 (2017).10.1016/j.jsb.2016.07.011CrossRefGoogle ScholarPubMed
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Himes, B. A. & Zhang, P. emClarity: software for high-resolution cryo-electron tomography and subtomogram averaging. Nat. Methods 15 , 955961 (2018).10.1038/s41592-018-0167-zCrossRefGoogle ScholarPubMed
Schur, F. K. M. et al. . An atomic model of HIV-1 capsid-SP1 reveals structures regulating assembly and maturation. Science 353 , 506508 (2016).10.1126/science.aaf9620CrossRefGoogle ScholarPubMed
Cassidy, C. K. et al. . CryoEM and computer simulations reveal a novel kinase conformational switch in bacterial chemotaxis signaling. Elife 4, 1193 (2015).10.7554/eLife.08419CrossRefGoogle ScholarPubMed
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