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Cryo-Electron Microscopy and Image Reconstruction of PBCV-1, an ALGAL Virus with T=169, Icosahedral Lattice Symmetry

Published online by Cambridge University Press:  02 July 2020

X. Yan ,
N. H. Olson ,
J. L. Van Etten  and
T. S. Baker
X. Yan
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN47907
N. H. Olson
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN47907
J. L. Van Etten
Affiliation:
Department of Plant Pathology, University of Nebraska, Lincoln, Nebraska68583
T. S. Baker
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN47907
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Extract

Paramecium bursaria Chlorella virus l(PBCV-l), the type species of the Pycodnaviridae family, is a large, plaque-forming virus that replicates in certain unicellular, exsymbiotic, Chlorella-like green algae. The virion (∼lxl09 daltons) has a 330,740 base pair dsDNA genome encapsulated within an icosahedral shell of ∼190nm diameter. Purified virions contain more than 50 different proteins, which account for 65% of the total virion mass and range in size from 10 to more than 200kDa. Vp54, the major capsid protein (40% of total virion protein) is a myristilated glycoprotein but it is not phosphorylated. Three other proteins are located on the virus surface. Virions contain 5-10% lipid, located inside the glycoprotein shell, which is required for virus infectivity. Little is known about the detailed ultrastructure of PBCV-1 virions, though some information has been obtained with sectioned, negatively-stained, and metal-shadowed specimens. We have used cryo-electron microscopy and three-dimensional (3D) image reconstruction methods to examine the native morphology of PBCV-1.

Type
Imaging of Macromolecular Complexes
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 948 - 949
Copyright
Copyright © Microscopy Society of America

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References

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