Hostname: page-component-848d4c4894-jbqgn Total loading time: 0 Render date: 2024-06-26T01:32:51.771Z Has data issue: false hasContentIssue false
  • English
  • Français

Cryo Ultramicrotomy of Biological Samples and Polymers

Published online by Cambridge University Press:  02 July 2020

H. Gnaegi
H. Gnaegi*
Affiliation:
Diatome ltd, P.O. Box 557, CH-2501Biel
Get access

Extract

Introduction

Cryo Ultramicrotomy gains increasing importance for immunocyto- and immunohisto- chemistry, element analysis, morphological studies and other investigations.

Major advances in immunocyto-chemistry and the sectioning of frozen hydrated specimens have been realized with the appropriate instrumentation and tools (1,2).

In immuno-electron microscopy it has been found that a considerable reduction of structural damage in tissue and cells can be obtained with a modified sectioning and pick-up method using sucrose/methyl cellulose solution (3, 4).

In recent years various scientists have tried to understand cryo sectioning and the problems related to the cry-osectioning process (5, 6, 7, 8).

A promising attempt to eliminate one of the major artefacts in ultramicrotomy, that of compression", was made with the development of the oscillating diamond knife (9).

In polymer research cryo ultramicrotomy is a well established technique.

However, only little information is available about the the cryo sectioning process for the following reasons: Much cryo sectioning is performed in polymer companies.

Type
Technologists Forum: Cryo Microscopy
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 314 - 315
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Sitte, H., Scanning Microscopy Supplement 10, 1996, 387–466.Google Scholar
2.Michel, M. et al., Journal of Microscopy, Vol. 166, Pt 1, 1992, 43–56.CrossRefGoogle Scholar
3.Tokuyasu, K. T., Journal of Cell Biology, Vol. 57, 1973, 551–565.CrossRefGoogle Scholar
4.Liu, W. et al., Histochemistry and Cell Biology, Vol. 106, 1996, 41–55.CrossRefGoogle Scholar
5.Chang, P. M. et al., Journal of Microscopy, Vol. 132, 1983, 109–123.CrossRefGoogle Scholar
6.Zierold, K., Ultramicroscopy, Vol. 14, 1984, 201–210.CrossRefGoogle Scholar
7.Richter, K. et al., Journal of Microscopy, Vol. 163, Pt 1, 1991, 19–28.CrossRefGoogle Scholar
8.Richter, K., Micron, Vol. 25, No. 4, 1994, 297–308.CrossRefGoogle Scholar
9.Studer, D. et al., Microscopy and Analysis 5, Suppl. 2: Proceedings, 1999, 418–419.Google Scholar
10.Vallotton, P. H. et al., J. Biomater. Sci. Polymer Edn., Vol 6, No.7, 1994, 609–620.CrossRefGoogle Scholar
11.Sawyer, L. C. et al., Polymer Microscopy, Second Edition, 1996, 102–122.CrossRefGoogle Scholar
12. Personal communication with B. Vastenhout, Dow Chemicals, Terneuzen, NL.Google Scholar