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Centrosome Proliferation in the Human Androgen-Responsive LNCaP and the Androgen-Independent DU145 Prostate Cancer Cell Lines

Published online by Cambridge University Press:  02 July 2020

Heide Schatten ,
Maureen Ripple ,
Ron Balczon ,
Meghan Taylor  and
Michael Crosser
Heide Schatten
Affiliation:
Department of Veterinary Pathobiology, University of Missouri-Columbia, Columbia, MO65211
Maureen Ripple
Affiliation:
University of Wisconsin Comprehensive Cancer Center, Department of Medicine, Environmental Toxicology Center, and the William S. Middleton Veterans Administration Hospital, University of Wisconsin, Madison, WI53792
Ron Balczon
Affiliation:
Department of Structural and Cellular Biology, The University of South Alabama, Mobile, AL36688
Meghan Taylor
Affiliation:
Department of Veterinary Pathobiology, University of Missouri-Columbia, Columbia, MO65211
Michael Crosser
Affiliation:
Department of Veterinary Pathobiology, University of Missouri-Columbia, Columbia, MO65211
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Extract

Cancer is a disease characterized by uncontrolled cell divisions in which the molecular controls for cytoskeletal regulation are bypassed. Cell division is governed by centrosomes, microtubule-organizing cell organelles which are crucial for the organization of the mitotic apparatus during mitosis and cell division. Because centrosome abnormalities are observed in the most common human cancers, we used immunofluorescence and transmission electron microscopy to determine centrosome organization in the human androgenresponsive prostate cancer cell line LNCaP and the androgen-independent prostate cancer cell line DU145. During interphase, centrosomes are located in close vicinity to the outer nuclear membrane, duplicate during S-phase, and become separated to the mitotic poles during the transition from interphase to mitosis. Centrosome regulation is based on a number of different factors which are only partly understood. Hormones play a role during developmental regulation of prostates which might trigger the activation of centrosome proteins and consequent cell divisions in order to ensure tissue growth.

Type
Pathology
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1066 - 1067
Copyright
Copyright © Microscopy Society of America

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References

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