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Cellular Invasion and Matrix Degradation, a Different Type of Matrix-Degrading Cells in the Cartilage of Catfish (Clarias gariepinus) and Japanese Quail Embryos (Coturnix coturnix japonica)

Part of: Micrographia Collection

Published online by Cambridge University Press:  04 October 2019

Soha A. Soliman ,
Basma Mohamed Kamal  and
Hanan H. Abd-Elhafeez Open the ORCID record for Hanan H. Abd-Elhafeez [Opens in a new window]
Soha A. Soliman
Affiliation:
Department of Histology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt
Basma Mohamed Kamal
Affiliation:
Anatomy and Embryology Department, Faculty of Veterinary Medicine, Sadat-City University, Sadat City, Egypt
Hanan H. Abd-Elhafeez*
Affiliation:
Department of Anatomy, Embryology and Histology, Assiut University, Assiut, 71526, Egypt
*
*Author for correspondence: Hanan H. Abd-Elhafeez, E-mail: hhmmzz91@gmail.com
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Abstract

We previously studied the phenomena of the mesenchymal cell-dependent mode of cartilage growth in quail and catfish. Thus, we selected the two cartilage models in which mesenchymal cells participate in their growth. In such models, cartilage degradation occurred to facilitate cellular invasion. The studies do not explain the nature of the cartilage degrading cells. The current study aims to explore the nature of the cartilage-degrading cells using transmission electron microscopy (TEM) and immunohistochemistry. Samples of cartilage have been isolated from the air-breathing organ of catfish and the cartilage of the prospective occipital bone of quail embryos. Samples have been processed for TEM and immunohistochemistry. We found that two different cell types are involved in cartilage degradation; the macrophage in the cartilage of catfish and mesenchymal cells in the cartilage of the quail. Areas of cellular invasion in both catfish cartilage and quail embryo cartilage had an immunological affinity for MMP-9. In catfish, cartilage-degrading cells had identical morphological features of macrophages, whereas in quail embryos, cartilage-degrading cells were mesenchymal-like cells which had cell processes rich in vesicles and expressed CD117. Further study should consider the role of macrophage and mesenchymal cells during cartilage degradation. This could be valuable to be applied to remove the defective cartilage matrix formed in osteoarthritic patients to improve cartilage repair strategies.

Keywords

c-kitcartilage degrading cellsmacrophagemesenchymal-like cellsMMP-9
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 25 , Issue 5 , October 2019 , pp. 1283 - 1292
Copyright
Copyright © Microscopy Society of America 2019 

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Footnotes

The work is equally distributed between authors.

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