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Autoschizic Death of Human Ovarian Carcinoma (MDAH 2774) Cells: LM and SEM Aspects

Published online by Cambridge University Press:  02 July 2020

J. Gillotcaux ,
J.M. Jamison ,
D. Arnold ,
V. Von Greunigen  and
J.L. Summers
J. Gillotcaux
Affiliation:
Lake Erie College of Osteopathic Medicine, Erie, PA, 1650
J.M. Jamison
Affiliation:
Urology, Summa Health System/NEOUCOM, Akron, OH44304
D. Arnold
Affiliation:
Lake Erie College of Osteopathic Medicine, Erie, PA, 1650
V. Von Greunigen
Affiliation:
Obstetrics and Gynecology, Akron General Hospital, Akron, OH, 44308, U.S.A.
J.L. Summers
Affiliation:
Urology, Summa Health System/NEOUCOM, Akron, OH44304
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Abstract

Co-administration of Vitamin C (VC) and Vitamin K3 (VK3) combination (in a VC/VK3 ratio of 100:1) to several human carcinoma cell lines resulted in equivalent antineoplastic activity at concentrations that were 10-50 times lower than when either vitamin was administered alone. Peroxides generated by VC resulted in membrane lipid alteration. VC and VK3 combination resulted in cytoskeletal alterations, DNAses and RNase activation. This report documents LM and SEM morphological changes of ovarian carcinoma cells which accompany vitamin treatment and lead to autoschizic cell death.

MDAH cells were cultivated in titer dishes and on coverslips coated with polylysine. After exposure to either VC, VK3, or the VC/VK3 combination for 1 hour, cells on coverslips were fixed and processed for SEM. Cells in dishes (105 cells) were harvested, centrifuged (<500 r/min), fixed and embedded in PolyBed. Sections, oneurn thick, were stained by Toluidine blue prior to LM and TEM studies.

Type
The Cell Biology of Cancer (Organized by J. Jerome and B. Gunning)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 586 - 587
Copyright
Copyright © Microscopy Society of America 2001

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