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Autoschizic Death of Human Ovarian Carcinoma (MDAH 2774) Cells: LM and SEM Aspects

Published online by Cambridge University Press:  02 July 2020

J. Gillotcaux
Affiliation:
Lake Erie College of Osteopathic Medicine, Erie, PA, 1650
J.M. Jamison
Affiliation:
Urology, Summa Health System/NEOUCOM, Akron, OH44304
D. Arnold
Affiliation:
Lake Erie College of Osteopathic Medicine, Erie, PA, 1650
V. Von Greunigen
Affiliation:
Obstetrics and Gynecology, Akron General Hospital, Akron, OH, 44308, U.S.A.
J.L. Summers
Affiliation:
Urology, Summa Health System/NEOUCOM, Akron, OH44304
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Abstract

Co-administration of Vitamin C (VC) and Vitamin K3 (VK3) combination (in a VC/VK3 ratio of 100:1) to several human carcinoma cell lines resulted in equivalent antineoplastic activity at concentrations that were 10-50 times lower than when either vitamin was administered alone. Peroxides generated by VC resulted in membrane lipid alteration. VC and VK3 combination resulted in cytoskeletal alterations, DNAses and RNase activation. This report documents LM and SEM morphological changes of ovarian carcinoma cells which accompany vitamin treatment and lead to autoschizic cell death.

MDAH cells were cultivated in titer dishes and on coverslips coated with polylysine. After exposure to either VC, VK3, or the VC/VK3 combination for 1 hour, cells on coverslips were fixed and processed for SEM. Cells in dishes (105 cells) were harvested, centrifuged (<500 r/min), fixed and embedded in PolyBed. Sections, oneurn thick, were stained by Toluidine blue prior to LM and TEM studies.

Type
The Cell Biology of Cancer (Organized by J. Jerome and B. Gunning)
Copyright
Copyright © Microscopy Society of America 2001

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References

1Noto, V. et al. Cancer 63 (1989) 9019063.0.CO;2-G>CrossRefGoogle Scholar
2Venugopal, M. et al. Life Science 59 (1996) 13891400CrossRefGoogle Scholar
3Taper, H.S. et al. Journal of Histochemistry and Cytochemistry 49 (2001) 109119CrossRefGoogle Scholar
4Gilloteaux, J. et al. Scanning 20 (1998) 564575CrossRefGoogle ScholarPubMed
5Ervin, E. et al. Scanning 20 (1998) 210211Google Scholar
6Gilloteaux, J. et al. Ultrastructural Patholology 25 (2001) in pressCrossRefGoogle Scholar