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Antibody-Mediated Self-Limiting Self-Assembly for Quantitative Analysis of Nanoparticle Surfaces by Atomic Force Microscopy

Published online by Cambridge University Press:  03 March 2011

Carly Lay A. Geronimo  and
Robert I. MacCuspie
Carly Lay A. Geronimo
Affiliation:
Nanomechanical Properties Group, National Institute of Standards and Technology, Gaithersburg, MD, USA
Robert I. MacCuspie*
Affiliation:
Nanomechanical Properties Group, National Institute of Standards and Technology, Gaithersburg, MD, USA
*
Corresponding author. E-mail: robert.maccuspie@nist.gov
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Abstract

Quantification of very low density molecular coatings on large (60 nm) gold nanoparticles (AuNPs) is demonstrated via the use of antibody-mediated self-limiting self-assembly of small and large AuNPs into raspberry-like structures subsequently imaged by atomic force microscopy (AFM). AFM imaging is proposed as an automated, lower-cost, higher-throughput alternative to immunostaining and imaging by transmission electron microscopy. Synthesis of large AuNPs, containing one of three ligand molecules in one of three stoichiometries (1, 2, or 10 ligands per AuNP), and small probe AuNPs with one of three antibody molecules in a one antibody per AuNP ratio, enabled a range of predicted self-limiting self-assembled structures. A model predicting the probability of observing a given small to large AuNP ratio based on a topography measurement such as AFM is described, in which random orientational deposition is assumed and which accounts for the stochastic synthesis method of the library AuNPs with varied ligand ratios. Experimental data were found to agree very well with the predictive models when using an established AFM sample preparation method that avoids drying-induced aggregation.

Keywords

self-limiting self-assemblynanoparticle surface characterizationimmunostainingatomic force microscopy (AFM)transmission electron microscopy (TEM)
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 17 , Issue 2 , April 2011 , pp. 206 - 214
Copyright
Copyright © Microscopy Society of America 2011

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Footnotes

NIST Disclaimer: Certain trade names and company products are mentioned in the text or identified in illustrations in order to specify adequately the experimental procedure and equipment used. In no case does such identification imply recommendation or endorsement by National Institute of Standards and Technology, nor does it imply that the products are necessarily the best available for the purpose.

References

REFERENCES

ASTM (2009). Interlaboratory Study to Establish Precision Statements for ASTM E2490-09 Standard Guide for measurement of particle size distribution of nanomaterials in suspension by photon correlation spectroscopy. Research Report E56-1001. West Conshohocken, PA: American Society for Testing and Materials.Google Scholar
Bonevich, J.E. & Haller, W.K. (2010). NIST—NCL Joint Assay Protocol, PCC-7: Measuring the size of nanoparticles using transmission electron microscopy (TEM). Gaithersburg, MD: National Institute of Standards and Technology.Google Scholar
Daniel, M.C. & Astruc, D. (2004). Gold nanoparticles: Assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Chem Rev 104, 293–346.Google Scholar
Dobrovolskaia, M.A., Patri, A.K., Zheng, J.W., Clogston, J.D., Ayub, N., Aggarwal, P., Neun, B.W., Hall, J.B. & McNeil, S.E. (2009). Interaction of colloidal gold nanoparticles with human blood: Effects on particle size and analysis of plasma protein binding profiles. Nanomed-Nanotechnol Biol Med 5, 106–117.Google Scholar
Duan, J., Park, K., MacCuspie, R.I., Vaia, R.A. & Pachter, R. (2009). Optical properties of rodlike metallic nanostructures: Insight from theory and experiment. J Phys Chem C 113, 15524–15532.CrossRefGoogle Scholar
Eck, W., Craig, G., Sigdel, A., Ritter, G., Old, L.J., Tang, L., Brennan, M.F., Allen, P.J. & Mason, M.D. (2008). PEGylated gold nanoparticles conjugated to monoclonal F19 antibodies as targeted labeling agents for human pancreatic carcinoma tissue. ACS Nano 2, 2263–2272.Google Scholar
Elghanian, R., Storhoff, J.J., Mucic, R.C., Letsinger, R.L. & Mirkin, C.A. (1997). Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles. Science 277, 1078–1081.CrossRefGoogle ScholarPubMed
El Rifai, O.M. & Youcef-Toumi, K. (2004). On automating atomic force microscopes: An adaptive control approach. Proceedings 43rd IEEE Conference on Decision and Control (CDC), Vols. 1–5, pp. 1574–1579. New York: Institute of Electrical and Electronics Engineers.Google Scholar
Fisher, T.E., Oberhauser, A.F., Carrion-Vazquez, M., Marszalek, P.E. & Fernandez, J.M. (1999). The study of protein mechanics with the atomic force microscope. Trends Biochem Sci 24, 379–384.Google Scholar
Gao, X.Y., Yu, L.T., MacCuspie, R.I. & Matsui, H. (2005). Controlled growth of Se nanoparticles on Ag nanoparticles in different ratios. Adv Mater 17, 426–429.Google Scholar
Grobleny, J., Delrio, F.W., Pradeep, N., Kim, D.-I., Hackley, V.A. & Cook, R.F. (2009). NIST—NCL Joint Assay Protocol, PCC-6: Size measurement of nanoparticles using atomic force microscopy. Gaithersburg, MD: National Institute of Standards and Technology. Available at http://ncl.cancer.gov/working_assay-cascade.asp.Google Scholar
Hackley, V.A. & Clogston, J.D. (2007). NIST—NCL Joint Assay Protocol PCC-1: Measuring the size of nanoparticles in aqueous media using batch-mode dynamic light scattering. Gaithersburg, MD: National Institute of Standards and Technology.Google Scholar
Hall, J.B., Dobrovolskaia, M.A., Patri, A.K. & McNeil, S.E. (2007). Characterization of nanoparticles for therapeutics. Nanomedicine 2, 789–803.Google Scholar
Hansen, D.J. (2008). FDA confronts nanotechnology. Chem Eng News 86, 32–34.CrossRefGoogle Scholar
Hostetler, M.J., Templeton, A.C. & Murray, R.W. (1999). Dynamics of place-exchange reactions on monolayer-protected gold cluster molecules. Langmuir 15, 3782–3789.Google Scholar
Jiang, K.Y., Schadler, L.S., Siegel, R.W., Zhang, X.J., Zhang, H.F. & Terrones, M. (2004). Protein immobilization on carbon nanotubes via a two-step process of diimide-activated amidation. J Mater Chem 14, 37–39.Google Scholar
Kelly, K.L., Coronado, E., Zhao, L.L. & Schatz, G.C. (2002). The optical properties of metal nanoparticles: The influence of size, shape, and dielectric environment. J Phys Chem B 107, 668–677.Google Scholar
Lee, C.K., Wang, Y.M., Huang, L.S. & Lin, S.M. (2007). Atomic force microscopy: Determination of unbinding force, off rate and energy barrier for protein-ligand interaction. Micron 38, 446–461.Google Scholar
Levy, R., Wang, Z.X., Duchesne, L., Doty, R.C., Cooper, A.I., Brust, M. & Fernig, D.G. (2006). A generic approach to monofunctionalized protein-like gold nanoparticles based on immobilized metal ion affinity chromatography. Chembiochem 7, 592–594.CrossRefGoogle ScholarPubMed
Link, S. & El-Sayed, M.A. (1999). Spectral properties and relaxation dynamics of surface plasmon electronic oscillations in gold and silver nanodots and nanorods. J Phys Chem B 103, 8410–8426.Google Scholar
Liu, J.Y. (2005). Scanning transmission electron microscopy and its application to the study of nanoparticles and nanoparticle systems. J Elec Microsc 54, 251–278.Google Scholar
MacCuspie, R.I., Allen, A.J. & Hackleky, V.A. (2011). Dispersion stabilization of silver nanoparticles in synthethetic lung fluid studied under in-situ conditions. Nanotoxicology doi:10.3109/17435390.2010.504311.Google Scholar
MacCuspie, R.I., Banerjee, I.A., Pejoux, C., Gummalla, S., Mostowski, H.S., Krause, P.R. & Matsui, H. (2008a). Virus assay using antibody-functionalized peptide nanotubes. Soft Matter 4, 833–839.CrossRefGoogle ScholarPubMed
MacCuspie, R.I., Elsen, A.M., Diamanti, S.J., Patton, S.T., Altfeder, I., Jacobs, J.D., Voevodin, A.A. & Vaia, R.A. (2010). Purification—chemical structure—electrical property relationship in gold nanoparticle liquids. Appl Organometal Chem 24, 590–599.Google Scholar
MacCuspie, R.I., Nuraje, N., Lee, S.Y., Runge, A. & Matsui, H. (2008b). Comparison of electrical properties of viruses studied by AC capacitance scanning probe microscopy. J Am Chem Soc 130, 887–891.Google Scholar
Maeshima, K., Eltsov, M. & Laemmli, U.K. (2005). Chromosome structure: Improved immunolabeling for electron microscopy. Chromosoma 114, 365–375.Google Scholar
Malinsky, M.D., Kelly, K.L., Schatz, G.C. & Van Duyne, R.P. (2001). Chain length dependence and sensing capabilities of the localized surface plasmon resonance of silver nanoparticles chemically modified with alkanethiol self-assembled monolayers. J Am Chem Soc 123, 1471–1482.Google Scholar
Markiewicz, P. & Goh, M.C. (1994). Atomic force microscopy probe tip visualization and improvement of images using a simple deconvolution procedure. Langmuir 10, 5–7.Google Scholar
Neffati, R., Alexeev, A., Saunin, S., Brokken-Zijp, J.C.M., Wouters, D., Schmatloch, S., Schubert, U.S. & Loos, J. (2003). Automated scanning probe microscopy as a new tool for combinatorial polymer research: Conductive carbon black/poly(dimethylsiloxane) composites. Macromolec Rapid Comm 24, 113–117.Google Scholar
Ngunjiri, J. & Garno, J.C. (2008). AFM-based lithography for nanoscale protein assays. Anal Chem 80, 1361–1369.Google Scholar
NIST (2008). Reference Material 8011, Gold Nanoparticles, Nominal 10 nm diameter. Gaithersburg, MD: National Institute of Standards and Technology.Google Scholar
Nuraje, N., Banerjee, I.A., Maccuspie, R.I., Yu, L.T. & Matsui, H. (2004). Biological bottom-up assembly of antibody nanotubes on patterned antigen arrays. J Am Chem Soc 126, 8088–8089.Google Scholar
Nuraje, N., Su, K., Samson, J., Haboosheh, A., MacCuspie, R.I. & Matsui, H. (2006). Self-assembly of Au nanoparticle-containing peptide nano-rings on surfaces. Supramolec Chem 18, 429–434.CrossRefGoogle ScholarPubMed
Paciotti, G.F., Myer, L., Weinreich, D., Goia, D., Pavel, N., McLaughlin, R.E. & Tamarkin, L. (2004). Colloidal gold: A novel nanoparticle vector for tumor directed drug delivery. Drug Delivery 11, 169–183.CrossRefGoogle ScholarPubMed
Patton, S.T., Slocik, J.M., Campbell, A., Hu, J.J., Naik, R.R. & Voevodin, A.A. (2008). Bimetallic nanoparticles for surface modification and lubrication of MEMS switch contacts. Nanotechnology 19, 405705–405711.Google Scholar
Ruben, G.C., Wang, J.Z., Iqbal, K. & Grundke-Iqbal, I. (2005). Paired helical filaments (PHFs) are a family of single filament structures with a common helical turn period: Negatively stained PHF imaged by TEM and measured before and after sonication, deglycosylation, and dephosphorylation. Microsc Res Techniq 67, 175–195.Google Scholar
Shaffer, A.W., Worden, J.G. & Huo, Q. (2004). Comparison study of the solution phase versus solid phase place exchange reactions in the controlled functionalization of gold nanoparticles. Langmuir 20, 8343–8351.Google Scholar
Sitti, M. (2003). Teleoperated and automatic nanomanipulation systems using atomic force microscope probes. Proceedings 42nd IEEE Conference on Decision and Control. Vols. 1–6, pp. 2118–2123. New York: Institute of Electrical and Electronics Engineers.Google Scholar
Slocik, J.M., Tam, F., Halas, N.J. & Naik, R.R. (2007). Peptide-assembled optically responsive nanoparticle complexes. Nano Lett 7, 1054–1058.Google Scholar
Tracy, J.B., Kalyuzhny, G., Crowe, M.C., Balasubramanian, R., Choi, J.P. & Murray, R.W. (2007). Poly(ethylene glycol) ligands for high-resolution nanoparticle mass spectrometry. J Am Chem Soc 129, 6706–6707.Google Scholar
Tsai, D.-H., Delrio, F.W., MacCuspie, R.I., Cho, T.J., Zachariah, M. & Hackley, V.A. (2010). Competitive adsorption of thiolated polyethylene glycol and mercaptopropionic acid on gold nanoparticles measured by physical characterization methods. Langmuir 26, 10325–10333.Google Scholar
Voevodin, A.A., Vaia, R.A., Patton, S.T., Diamanti, S., Pender, M., Yoonessi, M., Brubaker, J., Hu, J.J., Sanders, J.H., Phillips, B.S. & MacCuspie, R.I. (2007). Nanoparticte-wetted surfaces for relays and energy transmission contacts. Small 3, 1957–1963.Google Scholar
Williams, D.B. & Carter, C.B. (2009). Transmission Electron Microscopy: A Textbook for Materials Science. New York: Springer Science.Google Scholar
Woehrle, G.H., Brown, L.O. & Hutchison, J.E. (2005). Thiol-functionalized, 1.5-nm gold nanoparticles through ligand exchange reactions: Scope and mechanism of ligand exchange. J Am Chem Soc 127, 2172–2183.CrossRefGoogle ScholarPubMed
Woehrle, G.H. & Hutchison, J.E. (2005). Thiol-functionalized undecagold clusters by ligand exchange: Synthesis, mechanism, and properties. Inorganic Chem 44, 6149–6158.Google Scholar
Worden, J.G., Dai, Q., Shaffer, A.W. & Huo, Q. (2004). Monofunctional group-modified gold nanoparticles from solid phase synthesis approach: Solid support and experimental condition effect. Chem Mater 16, 3746–3755.Google Scholar
Xu, X.Y., Rosi, N.L., Wang, Y.H., Huo, F.W. & Mirkin, C.A. (2006). Asymmetric functionalization of gold nanoparticles with oligonucleotides. J Am Chem Soc 128, 9286–9287.Google Scholar
Yang, Y., Wang, H. & Erie, D.A. (2003). Quantitative characterization of biomolecular assemblies and interactions using atomic force microscopy. Methods 29, 175–187.Google Scholar
Zook, J.M., MacCuspie, R.I., Locascio, L.E. & Elliott, J.E. (2011). Stable nanoparticle aggregates/agglomerates of different sizes and the effect of their sizes on hemolytic cytotoxicity. Nanotoxicology doi:10.3109/17435390.2010.536615.Google Scholar
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