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Age-Dependent Acoustic and Microelastic Properties of Red Blood Cells Determined by Vector Contrast Acoustic Microscopy

Published online by Cambridge University Press:  29 May 2012

Esam T. Ahmed Mohamed ,
Albert E. Kamanyi ,
Mieczysław Pluta  and
Wolfgang Grill
Esam T. Ahmed Mohamed*
Affiliation:
Institute of Experimental Physics II, University of Leipzig, Linnéstr. 5, D-04103 Leipzig, Germany
Albert E. Kamanyi
Affiliation:
Institute of Experimental Physics II, University of Leipzig, Linnéstr. 5, D-04103 Leipzig, Germany
Mieczysław Pluta
Affiliation:
Institute of Experimental Physics II, University of Leipzig, Linnéstr. 5, D-04103 Leipzig, Germany
Wolfgang Grill
Affiliation:
Institute of Experimental Physics II, University of Leipzig, Linnéstr. 5, D-04103 Leipzig, Germany
*
Corresponding author. E-mail: esam@physik.uni-leipzig.de
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Abstract

Variations of the mechanical properties of red blood cells that occur during their life span have long been an intriguing task for investigations. The research presented is based on noninvasive monitoring of red blood cells of different ages performed by scanning acoustic microscopy with magnitude and phase contrast. The characteristic signature of fixed cells from groups of three different ages fractionated according to mass density is obtained from the acoustic microscope images, with the data represented in polar graphs. The analysis of these data enables the determination of averaged values for the velocities of ultrasound propagating in the cells from the different groups ranging from (1,681 ± 16) m s−1 in the youngest to (1,986 ± 20) m s−1 in the oldest group. The determined bulk modulus varies with age from (3.04 ± 0.05) GPa to (4.34 ± 0.08) GPa. An approach to determine for an age-mixed population of red blood cells, collected from a healthy person, the age of the individual cells and the age dependence of the cell parameters including density, velocity, and attenuation of longitudinal polarized ultrasonic waves traveling in the cells is demonstrated.

Keywords

SAMPSAMelastic properties of red blood cellsred blood cell age monitoring
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 18 , Issue 3 , June 2012 , pp. 436 - 444
Copyright
Copyright © Microscopy Society of America 2012

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