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Affinity Cryo-Electron Microscopy Studies of Viral Particles Captured Directly From Cell Culture

Published online by Cambridge University Press:  23 September 2015

Ci Zhang
Affiliation:
Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, West Lafayette, IN 47907
Frank Vago
Affiliation:
Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, West Lafayette, IN 47907
Fei Guo
Affiliation:
Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, West Lafayette, IN 47907
Zheng Liu
Affiliation:
Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, West Lafayette, IN 47907
Guimei Yu
Affiliation:
Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, West Lafayette, IN 47907
Phil Serwer
Affiliation:
Department of Biochemistry, University of Texas Health Science Center, San Antonio, TX 78229
Wen Jiang
Affiliation:
Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, West Lafayette, IN 47907
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Abstract

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Copyright © Microscopy Society of America 2015 

References

[1] Fang, PA, Wright, ET, Weintraub, ST, et al., Visualization of bacteriophage T3 capsids with DNA incompletely packaged in vivo. J Mol Biol 384 (2008). p 13841399.CrossRefGoogle ScholarPubMed
[2] Yu, G, Vago, F, Zhang, D, et al., Single-step antibody-based affinity cryo-electron microscopy for imaging and structural analysis of macromolecular assemblies. J Struct Biol 187 (2014). p. 19.CrossRefGoogle ScholarPubMed
[3] Llaguno, MC, Xu, H, Shi, L, et al., Chemically functionalized carbon films for single molecule imaging. J Struct Biol 185 (2014). p. 405417.CrossRefGoogle ScholarPubMed
[4] Han, BG, Walton, RW, Song, A, et al., Electron microscopy of biotinylated protein complexes bound to streptavidin monolayer crystals. J Struct Biol 180 (2012). p. 249253.CrossRefGoogle ScholarPubMed
[5] Kelly, DF, Dukovski, D & Walz, T Monolayer purification: a rapid method for isolating protein complexes for single-particle electron microscopy. Proc Natl Acad Sci U S A 105 (2008). p. 47034708.CrossRefGoogle ScholarPubMed
[6] Guo, F, Liu, Z, Fang, P, et al., Capsid expansion mechanism of bacteriophage T7 revealed by multi-state atomic models derived from cryo-EM reconstructions. Proceedings of the National Academy of Sciences of the United States of America 111 (2014). p. E460614.CrossRefGoogle Scholar
[7] Wikoff, WR, Liljas, L, Duda, RL, et al., Topologically linked protein rings in the bacteriophage HK97 capsid. Science 289 (2000). p. 21292133.CrossRefGoogle ScholarPubMed
[8] The authors acknowledge funding from the National Institutes of Health and Welch Foundation. The cryo-EM images were taken in the Purdue Cryo-EM Facility, and the Purdue Rosen Center for Advanced Computing provided the computational resource for the 3D reconstructions.Google Scholar
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