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Adding “colors” to cryo-EM: extracting local chemical data from radiation damage

Published online by Cambridge University Press:  30 July 2021

Gili Abelya ,
Leonard Joseph Campanello ,
Ran Zalk  and
Gabriel A Frank
Gili Abelya
Affiliation:
Dept. of Life Sciences, Ben-Gurion University of the Negev, United States
Leonard Joseph Campanello
Affiliation:
205 Orient St. Bayonne, NJ 07002, United States
Ran Zalk
Affiliation:
Ilse Katz Institute for Nanoscale Science & Technology, Ben-Gurion University of the Negev, United States
Gabriel A Frank
Affiliation:
Dept. of Life Sciences, Ben-Gurion University of the Negev, United States

Abstract

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Type
Michael Rossmann Memorial Symposium
Information
Microscopy and Microanalysis , Volume 27 , Supplement S1 , August 2021 , pp. 1386 - 1388
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

Baker, L. A.; Rubinstein, J. L. Radiation Damage in Electron Cryomicroscopy; Elsevier Masson SAS, 2010; Vol. 481. https://doi.org/10.1016/S0076-6879(10)81015-8.Google ScholarPubMed
Hattne, J.; Shi, D.; Glynn, C.; Martynowycz, M. W.; Rodriguez, J. A.; Correspondence, Gonen, T. Analysis of Global and Site-Specific Radiation Damage in Cryo-EM. Structure 2018, 26, 759–766. https://doi.org/10.1016/j.str.2018.03.021.CrossRefGoogle ScholarPubMed
Zivanov, J.; Nakane, T.; Forsberg, B.; Kimanius, D.; Hagen, W. J. H.; Lindahl, E.; Scheres, S. H. W. RELION-3: New Tools for Automated High-Resolution Cryo-EM Structure Determination. bioRxiv 2018, 1–22. https://doi.org/10.1101/421123.Google ScholarPubMed
Davidov, G.; Abelya, G.; Zalk, R.; Izbicki, B.; Shaibi, S.; Spektor, L.; Shagidov, D.; Meyron-Holtz, E. G.; Zarivach, R.; Frank, G. A. Folding of an Intrinsically Disordered Iron-Binding Peptide in Response to Sedimentation Revealed by Cryo-EM. Journal of the American Chemical Society 2020, 142 (46), 19551–19557. https://doi.org/10.1021/jacs.0c07565.CrossRefGoogle ScholarPubMed