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Quantitative Analysis of Metal Sequestering in Different Cell Components of Gloeocapsa alpicola in the Overplus Phase

Published online by Cambridge University Press:  02 July 2020

J.J. Goldberg  and
T.E. Jensen
J.J. Goldberg
Affiliation:
Department of Biological Sciences, Lehman College & CUNY Grad. Center, Bronx, NY10468
T.E. Jensen
Affiliation:
Department of Biological Sciences, Lehman College & CUNY Grad. Center, Bronx, NY10468
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Extract

The elemental composition of polyphosphate bodies (PPB's) and other components of the cell have previously been determined by using energy dispersive X-ray spectrometers(EDX). In this present study we perform a quantitative analysis of normally grown cells and cells that were grown in the overplus phenomenon.

Cells of Gloeocapsa alpicola were grown in modified Fitzgerald's media and harvested after a logarithmic growth phase of 14 days. The overplus cells were grown, as has been previously described. The cells were exposed to 20ppm of Al, Cd, Cu, Mn, Ni, Pb, and Zn, as well as a control, for one hour. The cells were then air dried on formvar coated grids or fixed and embedded in EPON according to Luft's procedure.

For Xn-ay analysis cells were first located using the TEM mode, and then the microscope was switched to the STEM mode. Analyses of cell components were carried out using the spot mode (75Kv).

Type
Biological Microanalysis
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 908 - 909
Copyright
Copyright © Microscopy Society of America

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References

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