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Presence of Caveolae in the Granular Epithelial Cells of Rabbit Urinary Bladder
Published online by Cambridge University Press: 02 July 2020
Extract
Previously, we reported the presence of caveolae, clathrin-coated pits and vesicles in toad urinary bladder granular epithelial cells, and in smooth muscle and endothelial cells. Since then we investigated the occurrence of caveolae in the rabbit urinary bladder cells. Here we report for the first time the presence of caveolae and clathrin-coated pits and vesicles in the granular epithelial, smooth muscle and endothelial cells of rabbit urinary bladders.
Urinary bladder sacs were surgically removed from doubly pithed toads and anaesthetized rabbits, and allowed fixation for 1 hr in 2% glutaraldehyde in PIPES buffer. These bladder tissues were divided for scanning (SEM) and transmission electron microscopy (TEM) for comparative analysis. A postfixation using 1% osmium tetroxide was carried out for 1 hr. Tissues were appropriately processed for SEM and TEM observations. Ultrathin sections, made with a diamond knife, were taken on bare nickel grids and then exposed to uranyl acetate and lead citrate for viewing in the TEM.
- Type
- Imaging Cells and Organelles
- Information
- Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 253 - 254
- Copyright
- Copyright © Microscopy Society of America 1997