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Observation of Amyloid-Like Fibers of the Sup35 Protein from Saccharomyces Cerevisiae

Published online by Cambridge University Press:  02 July 2020

Anthony S. Kowal ,
Thomas Scheibel  and
Susan L. Lindquist
Anthony S. Kowal
Affiliation:
Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois60637
Thomas Scheibel
Affiliation:
Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois60637
Susan L. Lindquist
Affiliation:
Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois60637
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Extract

In the yeast Saccharomyces cerevisiae, [PST] acts as an epigenetic modifier of translation termination efficiency. [PSI+] can be passed through generations of yeast cells via changes in protein conformation rather than changes in DNA or RNA, and has thus been referred to as a yeast prion. The [PSI+] determinant is the Sup35 protein. Sup35 can exist in two states - soluble and insoluble. Soluble Sup35 functions in translation termination, but when insoluble, stop codons are read through, resulting in incorrect protein products.

Sup35 is composed of three distinct domains, N, M, and C. The N region is rich in glutamine and asparagine and is required for the [PST] phenotype to exist. M is a highly charged domain, and no specific function has been assigned to it. C is essential in yeast, as it is responsible for translation termination. The insoluble form of Sup35 has characteristics reminiscent of other prion proteins - in vitro it binds to the dye Congo Red and it exhibits apple green birefringence in polarized light.

Type
Microorganisms: The Good, The Bad, The Unusual
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 664 - 665
Copyright
Copyright © Microscopy Society of America

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References

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