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JNK2 Participates in Spindle Assembly during Mouse Oocyte Meiotic Maturation

Published online by Cambridge University Press:  31 January 2011

Xin Huang
Affiliation:
Organ Transplantation Institute, Xiamen University, Xiamen City, Fujian Province, China State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Jing-Shan Tong
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Zhen-Bo Wang
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Cai-Rong Yang
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Shu-Tao Qi
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Lei Guo
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Ying-Chun Ouyang
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Song Quan
Affiliation:
Department of OB/GY, Southern Medical University, Guangzhou City, Guangdong Province, China
Qing-Yuan Sun
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Zhong-Quan Qi
Affiliation:
Organ Transplantation Institute, Xiamen University, Xiamen City, Fujian Province, China
Ru-Xin Huang*
Affiliation:
Organ Transplantation Institute, Xiamen University, Xiamen City, Fujian Province, China Health Bureau of Xiamen, Xiamen City, Fujian Province, China
Hai-Long Wang*
Affiliation:
Organ Transplantation Institute, Xiamen University, Xiamen City, Fujian Province, China
*Corresponding
Corresponding author. E-mail: rxhuang@public.xm.fj.cn
Corresponding author. E-mail: hailongwang@xmu.edu.cn

Abstract

It is well known that c-Jun N-terminal kinase (JNK) plays pivotal roles in various mitotic events, but its function in mammalian oocyte meiosis remains unknown. In this study, we found that no specific JNK2 signal was detected in germinal vesicle stage. JNK2 was associated with the spindles especially the spindle poles and cytoplasmic microtubule organizing centers at prometaphase I, metaphase I, and metaphase II stages. JNK2 became diffusely distributed and associated with the midbody at telophase I stage. Injection of myc-tagged JNK2α1 mRNA into oocytes also revealed its localization on spindle poles. The association of JNK2 with spindle poles was further confirmed by colocalization with the centrosomal proteins, γ-tubulin and Plk1. Nocodazole treatment showed that JNK2 may interact with Plk1 to regulate the spindle assembly. Then we investigated the possible function of JNK2 by JNK2 antibody microinjection and JNK specific inhibitor SP600125 treatment. These two manipulations caused abnormal spindle formation and decreased the rate of first polar body (PB1) extrusion. In addition, inhibition of JNK2 resulted in impaired localization of Plk1. Taken together, our results suggest that JNK2 plays an important role in spindle assembly and PB1 extrusion during mouse oocyte meiotic maturation.

Type
Biological Applications
Copyright
Copyright © Microscopy Society of America 2011

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