Hostname: page-component-76fb5796d-45l2p Total loading time: 0 Render date: 2024-04-26T17:54:12.159Z Has data issue: false hasContentIssue false
  • English
  • Français

A Gill Histopathology Study in two Native Fish Species from the Hydrographic Douro Basin

Published online by Cambridge University Press:  12 February 2019

Dércia Santos ,
Ana Luzio ,
Ana M. Coimbra Open the ORCID record for Ana M. Coimbra [Opens in a new window] ,
Simone Varandas ,
António Fontaínhas-Fernandes  and
Sandra M. Monteiro
Dércia Santos*
Affiliation:
Departamento de Biologia e Ambiente (DeBA), Centro de Investigação de Tecnologias Agro-Ambientais e Biológicas (CITAB), Escola de Ciências da Vida e Ambiente (ECVA), Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal
Ana Luzio
Affiliation:
Departamento de Biologia e Ambiente (DeBA), Centro de Investigação de Tecnologias Agro-Ambientais e Biológicas (CITAB), Escola de Ciências da Vida e Ambiente (ECVA), Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal
Ana M. Coimbra
Affiliation:
Departamento de Biologia e Ambiente (DeBA), Centro de Investigação de Tecnologias Agro-Ambientais e Biológicas (CITAB), Escola de Ciências da Vida e Ambiente (ECVA), Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal
Simone Varandas
Affiliation:
Departamento de Ciências Florestais e Arquitetura Paisagista (CIFAP), Centro de Investigação de Tecnologias Agro-Ambientais e Biológicas (CITAB), Escola de Ciências Agrárias e Veterinárias (ECAV), Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal
António Fontaínhas-Fernandes
Affiliation:
Departamento de Biologia e Ambiente (DeBA), Centro de Investigação de Tecnologias Agro-Ambientais e Biológicas (CITAB), Escola de Ciências da Vida e Ambiente (ECVA), Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal
Sandra M. Monteiro*
Affiliation:
Departamento de Biologia e Ambiente (DeBA), Centro de Investigação de Tecnologias Agro-Ambientais e Biológicas (CITAB), Escola de Ciências da Vida e Ambiente (ECVA), Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal
*
*Authors for correspondence: Dércia Santos, E-mail: dsantos@utad.pt; Sandra M. Monteiro, E-mail: smonteir@utad.pt
*Authors for correspondence: Dércia Santos, E-mail: dsantos@utad.pt; Sandra M. Monteiro, E-mail: smonteir@utad.pt
Get access

Abstract

Gill histopathology is a valuable tool to evaluate ecosystems health, because the gill is a key site of waterborne pollutant uptake and the first target organ to their toxicity. Consequently, this makes it important in biomonitoring programs. This study aims to evaluate gill histopathological differences in Douro basin native fish species and determine possible associations with water quality and the ecological status classifications. Two native fish species (Pseudochondrostoma duriense and Luciobarbus bocagei) were sampled in four points of the Douro basin: two reference points, Ameixiosa (Paiva River) and Covelas (Bestança River), both classified with an excellent ecological status; and two disturbed points, Castro Daire (Paiva River) and Alvações do Corgo (Corgo River), categorized with a good and a moderate ecological status, respectively. Gill histopathological differences were qualitative and quantitatively analyzed. The histological analysis showed that, in all sampling locations, both species presented some degree of gill differences, such as epithelial lifting, lamellar fusion, and/or necrosis. The histopathological differences evaluation emphasized some variances in the responses between the two species. In nase, the filament and lamellar epithelium proliferation were the histopathological differences that better reflected the river ecological status classification, proving their usefulness in biomonitoring programs.

Keywords

biomonitoringfreshwater fisheshistologyLuciobarbus bocageiPseudochondrostoma duriense
Type
Life Sciences
Information
Microscopy and Microanalysis , Volume 25 , Issue 1 , February 2019 , pp. 236 - 243
Copyright
Copyright © Microscopy Society of America 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abdel-Moneim, AM, Al-Kahtani, MA and Elmenshawy, OM (2012) Histopathological biomarkers in gills and liver of Oreochromis niloticus from polluted wetland environments, Saudi Arabia. Chemosphere 88(8), 1028–1035.Google Scholar
Agamy, E (2013) Sub chronic exposure to crude oil, dispersed oil and dispersant induces histopathological alterations in the gills of the juvenile rabbit fish (Siganus canaliculatus). Ecotoxicol Environ Saf 92, 180–190.Google Scholar
Ameur, WB, De Lapuente, J, El Megdiche, Y, Barhoumi, B, Trabelsi, S, Camps, L, Serret, J, Ramos-Lopez, D, Gonzalez-Linares, J, Driss, MR and Borras, M (2012) Oxidative stress, genotoxicity and histopathology biomarker responses in mullet (Mugil cephalus) and sea bass (Dicentrarchus labrax) liver from Bizerte Lagoon (Tunisia). Mar Pollut Bull 64(2), 241–251.Google Scholar
Au, DW (2004) The application of histo-cytopathological biomarkers in marine pollution monitoring: A review. Mar Pollut Bull 48(9–10), 817–834.Google Scholar
Barbieri, E, Campos-Garcia, J, Martinez, DS, Da Silva, JR, Alves, OL and Rezende, KF (2016) Histopathological effects on gills of Nile Tilapia (Oreochromis niloticus, Linnaeus, 1758) exposed to Pb and carbon nanotubes. Microsc Microanal 22(6), 1162–1169.Google Scholar
Barisic, J, Dragun, Z, Ramani, S, Filipovic Marijic, V, Krasnici, N, Coz-Rakovac, R, Kostov, V, Rebok, K and Jordanova, M (2015) Evaluation of histopathological alterations in the gills of Vardar chub (Squalius vardarensis Karaman) as an indicator of river pollution. Ecotoxicol Environ Saf 118, 158–166.Google Scholar
Bernet, D, Schmidt, H, Meier, W, Burkhardt-Holm, P and Wahli, T (1999) Histopathology in fish: Proposal for a protocol to assess aquatic pollution. J Fish Dis 22(1), 25–34.Google Scholar
Bordalo, AA, Teixeira, R and Wiebe, WJ (2006) A water quality index applied to an international shared river basin: The case of the Douro river. Environ Manage 38, 910–920.Google Scholar
Colin, N, Porte, C, Fernandes, D, Barata, C, Padros, F, Carrasson, M, Monroy, M, Cano-Rocabayera, O, De Sostoa, A, Pina, B and Maceda-Veiga, A (2016) Ecological relevance of biomarkers in monitoring studies of macro-invertebrates and fish in Mediterranean rivers. Sci Total Environ 540, 307–323.Google Scholar
Dane, H and Sisman, T (2015) Histopathological changes in gill and liver of Capoeta capoeta living in the Karasu River, Erzurum. Environ Toxicol 30(8), 904–917.Google Scholar
European Union. (2000) Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy. Off J Eur Union L327, 1–73.Google Scholar
Evans, DH (1987) The fish gill: Site of action and model for toxic effects of environmental pollutants. Environ Health Perspect 71, 47–58.Google Scholar
Evans, DH, Piermarini, PM and Choe, KP (2005) The multifunctional fish gill: Dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste. Physiol Rev 85(1), 97–177.Google Scholar
Fernandes, C, Fontainhas-Fernandes, A, Monteiro, SM and Salgado, MA (2007) Histopathological gill changes in wild leaping grey mullet (Liza saliens) from the Esmoriz-Paramos coastal lagoon, Portugal. Environ Toxicol 22(4), 443–448.Google Scholar
INAG (2009) Critérios para a Classificação do Estado das Massas de Água Superficiais – Rios e Albufeiras. Ministério do Ambiente, do Ordenamento do Território e do Desenvolvimento Regional. Instituto da Água, I. P. 29 pp.Google Scholar
Malaj, E, Von Der Ohe, PC, Grote, M, Kuhne, R, Mondy, CP, Usseglio-Polatera, P, Brack, W and Schafer, RB (2014) Organic chemicals jeopardize the health of freshwater ecosystems on the continental scale. Proc Natl Acad Sci USA 111(26), 9549–9554.Google Scholar
Mallatt, J (1985) Fish gill structural changes induced by toxicants and other irritants: A statistical review. Can J Fish Aquat Sci 42(4), 630–648.Google Scholar
Mazon, AF, Cerqueira, CC and Fernandes, MN (2002) Gill cellular changes induced by copper exposure in the South American tropical freshwater fish Prochilodus scrofa. Environ Res 88(1), 52–63.Google Scholar
Monteiro, SM, Rocha, E, Fontaínhas-Fernandes, A and Sousa, M (2008) Quantitative histopathology of Oreochromis niloticus gills after copper exposure. J Fish Biol 73(6), 1376–1392.Google Scholar
Mucha, AP, Bordalo, AA and Vasconcelos, MT (2004) Sediment quality in the Douro river estuary based on trace metal contents, macrobenthic community and elutriate sediment toxicity test (ESTT). J Environ Monit 6(7), 585–592.Google Scholar
Pereira, S, Pinto, AL, Cortes, R, Fontainhas-Fernandes, A, Coimbra, AM and Monteiro, SM (2013) Gill histopathological and oxidative stress evaluation in native fish captured in Portuguese northwestern rivers. Ecotoxicol Environ Saf 90(0), 157–166.Google Scholar
Raven, PJ, Fox, P, Everard, M, Holmes, NTH and Dawson, FH (1997) River habitat survey: A new system for classifying rivers according to their habitat quality. In Freshwater Quality: Defining the Indefinable?, Boon, PJ and Howell, DL (Eds.). The Stationery Office, Edinburgh, pp. 215–234.Google Scholar
Raven, PJ, Holmes, NTH, Dawson, FH and Everard, M (1998) Quality assessment using river habitat survey data. Aquat Conserv Mar Freshw Ecosyst 8, 477–499.Google Scholar
Rocha, MJ, Cruzeiro, C, Ferreira, C and Rocha, E (2012) Occurrence of endocrine disruptor compounds in the estuary of the Iberian Douro River and nearby Porto Coast (NW Portugal). Toxicol Environ Chem 94, 252–261.Google Scholar
Schlacher, TA, Mondon, JA and Connolly, RM (2007) Estuarine fish health assessment: Evidence of wastewater impacts based on nitrogen isotopes and histopathology. Mar Pollut Bull 54(11), 1762–1776.Google Scholar
Van Der Oost, R, Beyer, J and Vermeulen, NP (2003) Fish bioaccumulation and biomarkers in environmental risk assessment: A review. Environ Toxicol Pharmacol 13(2), 57–149.Google Scholar
Whitfield, AK and Elliott, M (2002) Fishes as indicators of environmental and ecological changes within estuaries: A review of progress and some suggestions for the future. J Fish Biol 61(sa), 229–250.Google Scholar
Yancheva, V, Velcheva, I, Stoyanova, S and Georgieva, E (2016) Histological biomarkers in fish as a tool in ecological risk assessment and monitoring programs: A review. Appl Ecol Environ Res 14(1), 47–75.Google Scholar