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Fluorescence Intravital Microscopy of the Coronary Microvasculature
Published online by Cambridge University Press: 02 July 2020
Extract
Much of our knowledge of coronary blood flow regulation is based on global blood flow measurements or from studies of large epicardial coronary arteries. Those studies which have examined the coronary microcirculation, have often considered it as a single homogeneous vascular bed. However, regulation of coronary microvascular resistance is not distributed uniformly, but varies across different segments of the vasculature. Under normal conditions, a major portion of coronary vascular resistance is controlled by the coronary arterioles.
Studies of these vessels in vivo are difficult, due to problems associated with cardiac and ventilatory motion. To eliminate these problems, we have utilized a technique of fluorescent stroboscopic epi-illumination. Dogs (4-12 kg) were anesthetized with sodium pentobarbital, intubated, and ventilated with room air. A 24 gauge catheter was placed in the proximal left circumflex coronary artery for measurements of coronary artery pressure and administration of drugs. Following these procedures, the animal was ventilated on a high frequency jet ventilator synchronized to the cardiac cycle.
- Type
- From Scanning Probe Microscopy to High Resolution Ultrasound: New Versions of the Vasculature
- Information
- Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 321 - 322
- Copyright
- Copyright © Microscopy Society of America 1997
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