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Analysis of Biological Structures by Electron Energy Loss Spectroscopy and Imaging

Published online by Cambridge University Press:  02 July 2020

R.D. Leapman  and
S.B. Andrews
R.D. Leapman
Affiliation:
Division of Bioengineering and Physical Science, ORS, MD20892
S.B. Andrews
Affiliation:
Laboratory of Neurobiology, NINDS, National Institutes of Health, Bethesda, MD20892
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Abstract

As techniques for electron energy-loss spectroscopy (EELS) reach a higher degree of optimization, detection limits for analyzing biological structures are approaching those predicted by theory. in favorable specimens, single atom detection is predicted for elemental maps acquired by means of the scanning transmission electron microscope (STEM) equipped with a field emission source, paralleldetection EELS and a spectrum-imaging system. to obtain such results, the electron detector should have a detective quantum efficiency close to unity and a well behaved point-spread function; such design features are now available with a cooled charge-couple device (CCD) array. The energy-filtering transmission electron microscope (EFTEM) provides a complementary approach to mapping elements occurring at higher concentrations but distributed over larger regions of the specimen. Use of an optimized CCD detector in the EFTEM now enables accurate quantitation in addition to high analytical sensitivity, albeit not at the single atom level.

Type
EELS Microanalysis at High Sensitivity: Advances in Spectrum Imaging, Energy Filtering and Detection (Organized by R. Leapman and J. Bruley)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 1166 - 1167
Copyright
Copyright © Microscopy Society of America 2001

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References

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