The species of the Parmelia saxatilis complex occurring in the Iberian Peninsula were revised. Eight species are accepted, including a new species found in southern Spain, described as P. rojoi A. Crespo, V. J. Rico & Divakar. The new species, which forms a sister-group relationship with P. saxatilis s. str., is rare in the Iberian Peninsula and is restricted to higher altitudes of northern and central Spain. Parmelia rojoi differs from P. saxatilis by generally narrower isidia and a more fragile thallus. The segregation of the new species is also supported by ITS (rDNA) and Mcm7 (MS456) phylogeny and multispecies coalescent-based approaches, including StarBEAST and BP&P. Furthermore, the divergence of P. rojoi is dated back to the Pleistocene, c. 2.13 Ma. A key to the identification of species from the P. saxatilis complex with their diagnostic features is provided. All species of the complex known from Europe are also found in the Iberian Peninsula. We hypothesize that P. rojoi is a relict species that survived the Pleistocene glaciations in refugia in Spain and has been unable to extend its distributional range in postglacial periods.

Saxicolous, lecideoid lichenized fungi have a cosmopolitan distribution but, being mostly cold adapted, are especially abundant in polar and high-mountain regions. To date, little is known of their origin or the extent of their trans-equatorial dispersal. Several mycobiont genera and species are thought to be restricted to either the Northern or the Southern Hemisphere, whereas others are thought to be widely distributed and occur in both hemispheres. However, these assumptions often rely on morphological analyses and lack supporting molecular genetic data. Also unknown is the extent of regional differentiation in the southern polar regions. An extensive set of lecideoid lichens (185 samples) was collected along a latitudinal gradient at the southern end of South America. Subantarctic climate conditions were maintained by increasing the elevation of the collecting sites with decreasing latitude. The investigated specimens were placed in a global context by including Antarctic and cosmopolitan sequences from other studies. For each symbiont three markers were used to identify intraspecific variation (mycobiont: ITS, mtSSU, RPB1; photobiont: ITS, psbJ-L, COX2). For the mycobiont, the saxicolous genera Lecidea, Porpidia, Poeltidea and Lecidella were phylogenetically re-evaluated, along with their photobionts Asterochloris and Trebouxia. For several globally distributed species groups, the results show geographically highly differentiated subclades, classified as operational taxonomical units (OTUs), which were assigned to the different regions of southern South America (sSA). Furthermore, several small endemic and well-supported clades apparently restricted to sSA were detected at the species level for both symbionts.

Five species of lichenicolous fungi are described as new to science: Buelliella ohmurae Zhurb. & Diederich (on Icmadophila), with a non-granulose epihymenium, not or only slightly enlarged, pale brown apical cells of paraphysoids and 1-septate, smooth ascospores; Catillaria japonica Zhurb. & Hafellner (on Dibaeis and Pseudobaeomyces), with a medium to dark reddish brown epihymenium, exciple and hypothecium, rather frequently branched and anastomosed paraphyses with only slightly enlarged apical cells without a dark cap, and Catillaria-type asci; Cryptodiscus ihlenii Zhurb. (on Dibaeis), with persistently immersed ascomata, non-amyloid asci and hymenium, not or only slightly enlarged apical cells of paraphyses and narrowly obovate, 1-septate ascospores; Llimoniella chilensis Zhurb. (on Dibaeis and a sterile microsquamulose lichen), with a K+ green exciple and epihymenium and aseptate, broadly ellipsoid ascospores; and Stigmidium phyllobaeidis Zhurb., Etayo & Flakus (on apothecial discs of Phyllobaeis), with a hemiamyloid interascal gel, not previously reported in that genus, well-developed, 1‒2(‒4)-celled periphysoids, elongate asci and hyaline, 1-septate ascospores. An undescribed species of Arthonia (on Pseudobaeomyces) is briefly characterized. Sphaerellothecium coniodes is newly reported for Asia. A key to the 32 species of lichenicolous fungi and lichens known to occur on baeomycetoid lichens and Icmadophila is provided.

The phylogeny of foliicolous taxa in Pilocarpaceae was reconstructed using Bayesian and maximum likelihood analyses of concatenated ITS and mtSSU sequences. Sixty-six new partial sequences representing 36 taxa were generated and 29 sequences were downloaded from GenBank. Our results indicate that Lasioloma R. Sant. is nested within a paraphyletic Calopadia Vězda, whereas Fellhanera Vězda is polyphyletic and the phylogenetic relationships of Eugeniella Lücking et al. and Sporopodium Mont. with Fellhanera require further investigation. In addition, Tapellaria parvimuriformis W. C. Wang & J. C. Wei is described as a new species from Khao Yai National Park, Thailand, and is characterized by its small muriform ascospores, grey campylidia with a pale base, and short conidia. Eight new records of foliicolous lichens for Thailand are also listed.

Common gardens are experimental plantations for comparing the performance of tree species while eliminating many of the variables that prevail in natural tree stands. The aim of this study was to evaluate the biodiversity of corticolous lichens on Danish tree species (Acer pseudoplatanus, Alnus glutinosa, Betula pendula, Fagus sylvatica, Fraxinus excelsior, Quercus robur and Tilia cordata) under common garden conditions and to examine the height distribution of particular lichen species. Observations were recorded through regular sampling of at least 36 lichen species on the main stems (from the base of the stem to the treetops) of 44-year-old trees at four common garden sites. Acer pseudoplatanus and Fraxinus excelsior had the greatest lichen species richness and Shannon diversity values while these measures were significantly lower for Betula pendula and Fagus sylvatica. The distribution of lichen species appeared biased among tree species. The general lichen distribution and relative sample height were weakly related (nonmetric multidimensional scaling). However, single lichen species showed a clear differential distribution along the tree stem (P < 0.001, non-parametric multiplicative regression and logistic log-binomial regression). Lepraria incana, Pseudosagedia aenea and Arthonia atra were mainly found at the stem base while Lecanora carpinea, L. chlarotera, Lecidella elaeochroma, Physcia tenella and Xanthoria parietina, were most abundant at around 70% of the total tree height. The differential distribution of single lichen species presumably reflects different specific requirements during spore germination and thallus growth. By isolating the unique effect of key variables (tree species and height), this study contributes to the knowledge base of corticolous lichen ecology.

The principal goal of our study was to test whether ecologically and chemically different populations of lichens in the Physconia muscigena group belong to a single, or multiple, species. We used sequence data from three markers (ITS rDNA, mtSSU rDNA and TEF1-α) for the reconstruction of phylogenetic trees based on a sampling of mostly European and Canadian populations of P. muscigena (Ach.) Poelt, P. muscigena var. bayeri (Nádv.) Poelt and P. isidiomuscigena Essl. In addition, we sought any possible geographical or ecological trends among chemotypes and haplotypes. Results show that: 1) sequence data of ITS rDNA and TEF1-α show large genetic variation in the Physconia muscigena group, which does not correlate with geographical distribution or thallus chemistry; 2) Physconia muscigena var. bayeri and P. isidiomuscigena appear undifferentiated with P. muscigena in our phylogenetic trees, and the three species cannot be distinguished on the basis of ITS rDNA, mtSSU rDNA and TEF1-α sequences. We therefore synonymized Physconia muscigena var. bayeri with P. muscigena and we recombine P. isidiomuscigena as a variety of P. muscigena.

A new species, Huriella flakusii, is described from the arid region of the Colca Canyon in southern Peru, and is characterized by squamulose, orange thalli and abundant, crowded and aggregated, usually flexuous when compressed, reddish apothecia. The thalline cortex, proper margin and hypothecium are paraplectenchymatous. The ascospores are broadly ellipsoid, 10‒15 × 5.0‒9.5 µm, with rather thin septa, 2‒4 µm. Phylogenetic analyses of the DNA sequences of ITS, nuLSU and mrSSU rDNA markers revealed that the new species is nested within the Amundsenia-Squamulea subclade in the subfamily Xanthorioideae, and has a sister group relationship to Huriella loekoesiana from South Korea.

Fruticose lichens of the genus Usnea Dill. ex Adans. (Parmeliaceae), generally known as beard lichens, are among the most iconic epiphytic lichens in modern forest ecosystems. Many of the c. 350 currently recognized species are widely distributed and have been used as bioindicators in air pollution studies. Here we demonstrate that usneoid lichens were present in the Palaeogene amber forests of Europe. Based on general morphology and annular cortical fragmentation, one fossil from Baltic amber can be assigned to the extant genus Usnea. The unique type of cortical cracking indirectly demonstrates the presence of a central cord that keeps the branch intact even when its cortex is split into vertebrae-like segments. This evolutionary innovation has remained unchanged since the Palaeogene, contributing to the considerable ecological flexibility that allows Usnea species to flourish in a wide variety of ecosystems and climate regimes. The fossil sets the minimum age for Usnea to 34 million years (late Eocene). While the other similar fossils from Baltic and Bitterfeld ambers cannot be definitely assigned to the same genus, they underline the diversity of pendant lichens in Palaeogene amber forests.

A molecular phylogeny of the genus Byssoloma is inferred from mtSSU sequences using Bayesian and maximum likelihood phylogenetic analyses. Byssoloma subdiscordans is resolved as sister to B. citricola rather than to the B. leucoblepharum clade, the B. subundulatum group (species with a compact apothecial margin) is shown to be a monophyletic group, and three species belonging to this group are described as new to science from Hainan Province in China: B. brunneodiscum W. C. Wang & J. C. Wei, with dark brown apothecia, crystals in the excipulum and the presence of 2,5,7-trichloro-3-O-methylnorlichexanthone; B. rubrofuscum W. C. Wang & J. C. Wei, with red-brown apothecia, 3–6-septate ascospores and the presence of 5,7-dichloro-3-O-methylnorlichexanthone; B. melanodiscocarpum W. C. Wang & J. C. Wei, with pure black apothecia, a K+ olive-black hypothecium and the presence of thiophanic acid.

Fungi are one of the most diverse carbon source-assimilating organisms, living as saprobes, parasites and symbionts; they play an important role in carbon cycling in the ecosystem. A lichen thallus provides habitats for many non-lichenized fungi and usually contains large quantities of polyols. However, research has not been undertaken to identify carbon sources of lichen-inhabiting fungi. In this study, we isolated various lichen-inhabiting fungi from surface-sterilized Ramalina spp., Flavoparmelia caperata and Peltigera degenii, and demonstrated their ability to assimilate carbon sources, namely glucose, ribitol and mannitol. Several isolates efficiently assimilated mannitol and ribitol; however, most isolates could assimilate only mannitol or both ribitol and mannitol at low levels. It is suggested that there are different preferences and niche segregation of carbon sources among lichen-inhabiting fungi, and that this assemblage includes fungi with different lifestyles such as saprobes, endophytes and transient visitors.

Corticolous lichens in the central mountains of Sri Lanka differ with vegetation type, disturbance and climate. All growth forms of lichens were studied in 42 plots (six plots × seven vegetation types), yielding 124 species. Lichen species diversity varied with number of tree species per plot (correlations) and differed with disturbance group, vegetation type and climate zone (general linear models). Lichen community composition (estimated cover of 74 species each at ≥ 3 plots) varied along two ordination gradients secondarily correlated with disturbance (nonmetric multidimensional scaling, NMS). Undisturbed and disturbed plots (mostly grouped by vegetation type) were divided along NMS axis 1, correlating with distance to undisturbed forest. Longest-disturbed plots differed from all others along NMS axis 2 and were correlated with canopy cover. Climate was weakly reflected on the ordination as the proximity of two plot clusters in montane vegetation types. Indicator species analyses (ISA) of lichen cover by plot identified 60 strong indicator species (indicator value ≥ 50%, P < 0.015). Fifty-seven species were indicators for individual vegetation types (28 of them for undisturbed types); three were for a disturbance group only; 11 were also for a disturbance group or climate zone. Most species strongly driving ordination patterns were also ISA indicators. Most lichens were crustose (39, with 24 in the Graphidaceae). Each vegetation type had at least one indicator with trentepohliod algae (increasing for undisturbed plots) and one with chlorococcoid algae. Two visually distinct indicator species, three genera and two multi-genus groups will be useful to parataxonomists in forest evaluation.

Usnic acid is a unique lichen metabolite of industrial importance, widely studied to explore its pharmacological potential and valued especially as an antibacterial agent in cosmetics. Although a vast number of papers describe usnic acid extraction from various lichen species, none has so far provided an unequivocal indication of the best extraction procedure for this compound. Thus, the current study was focused on the direct comparison of three commonly used usnic acid extraction methods (heat reflux, shaking, ultrasound-assisted extractions), which were optimized using fractional factorial design. Heat reflux extraction, shaking extraction and ultrasound-assisted extraction were first optimized in a series of experiments using fractional factorial design, with respect to three parameters: the extraction time, the solvent used and the number of extraction repetitions. HPLC was employed for usnic acid quantitative analysis. The best scores for each extraction method were statistically compared and the optimal conditions were indicated. The optimal set of parameters for usnic acid was established to be a single, 60 min heat reflux extraction with acetone. This extraction scheme provided 4.25 ±0.08 mg g−1 d.w. of usnic acid, while for ultrasound-assisted and shaking extractions the amount was two- or even four times lower (2.33 ±0.17 and 0.97 ±0.08 mg g−1 d.w., respectively). The optimal procedure for usnic acid extraction described here may be suitable for effective acquisition of this compound for scientific research purposes, but also for applications in the pharmaceutical or cosmetic industries.