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Verrucariaceae from Nepal

Published online by Cambridge University Press:  29 July 2022

Alan Orange*
Honorary Research Fellow, Natural Sciences, Amgueddfa Cymru - National Museum Wales, Cardiff CF10 3NP, Wales, UK
Som G. Chhetri
Ratnanagar 6, Bachhauli, Chitwan, Nepal
Author for correspondence: Alan Orange. E-mail:


Twenty-eight species of Verrucariaceae are reported from Nepal. One genus and nine species are described as new: Nesothele gen. nov., sister to Staurothele s. lat., with a crustose to squamulose thallus, hymenial algae and 4–8 colourless muriform ascospores per ascus; Nesothele glebulosa sp. nov., resembling N. rugulosa but with smaller perithecia; Thelidium uvidulum sp. nov., producing a thin thallus with soralia, prominent perithecia, and 1-septate ascospores; Verrucaria antepotens sp. nov., having a well-developed thallus with dark-sided areoles, immersed perithecia, and small ascospores 12.5–16.5 μm long; V. lactea sp. nov., resembling V. praetermissa but with larger ascospores and a strongly deviating ITS sequence; V. parvipeltata sp. nov., with brown, basally constricted areoles on an extensive dark prothallus; V. senta sp. nov., with a brown cracked thallus and prominent naked perithecia; Willeya eminens sp. nov., with prominent perithecia, and differing from W. protrudens in its ITS sequence; W. irrigata sp. nov. with a thallus cracked into dark-sided areoles, and relatively large ascospores 28.5–40 μm long; W. nepalensis sp. nov. with a cracked thallus, immersed perithecia and a distinctive ITS sequence. Three species complexes might contain new taxa, but wider geographical sampling is necessary before delimiting species: Thelidium minutulum, Verrucaria elaeomelaena and V. hydrophila. Five new combinations are made: Willeya honghensis comb. nov. (for Staurothele honghensis), Nesothele globosa comb. nov. (for Endocarpon globosum), N. hymenogonia comb. nov. (for Staurothele hymenogonia), N. rugulosa comb. nov. (for Staurothele rugulosa) and N. succedens comb. nov. (for Staurothele succedens). Four species are unidentified.

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Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of the British Lichen Society

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Aptroot, A (2016) The first European Willeya (Verrucariaceae) on limestone brought from China. Herzogia 29, 688691.CrossRefGoogle Scholar
Aptroot, A and Feijen, FJ (2002) Annotated checklist of the lichens and lichenicolous fungi of Bhutan. Fungal Diversity 11, 2148.Google Scholar
Aptroot, A and Sparrius, LB (2006) Additions to the lichen flora of Vietnam, with an annotated checklist and bibliography. Bryologist 109, 358371.CrossRefGoogle Scholar
Awasthi, DD (1991) A key to the microlichens of India, Nepal and Sri Lanka. Bibliotheca Lichenologica 40, 1337.Google Scholar
Borchsenius, F (2009) FastGap 1.2. Department of Biosciences, Aarhus University. [WWW resource] URL Scholar
Breuss, O (1996) Revision der Flechtengattung Placidiopsis (Verrucariaceae). Österreissiche Zeitschrift für Pilzkunde 5, 6594.Google Scholar
Buaruang, K, Boonpragob, K, Mongkolsuk, P, Sangvichien, E, Vongshewarat, K, Polyiam, W, Rangsiruji, A, Saipunkaew, W, Naksuwankul, K, Kalb, J, et al. (2017) A new checklist of lichenized fungi occurring in Thailand. MycoKeys 23, 191.CrossRefGoogle Scholar
Döring, H, Clerc, P, Grube, M and Wedin, M (2000) Mycobiont-specific PCR primers for the amplification of nuclear ITS and LSU rDNA from lichenized ascomycetes. Lichenologist 32, 200204.CrossRefGoogle Scholar
Gardes, M and Bruns, TD (1993) ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts. Molecular Ecology 2, 113118.CrossRefGoogle Scholar
Gueidan, C, Roux, C and Lutzoni, F (2007) Using a multigene phylogenetic analysis to assess generic delineation and character evolution in Verrucariaceae (Verrucariales, Ascomycota). Mycological Research 111, 11451168.CrossRefGoogle Scholar
Gueidan, C, Savić, S, Thüs, H, Roux, C, Keller, C, Tibell, L, Prieto, M, Heiðmarsson, S, Breuss, O, Orange, A, et al. (2009) Generic classification of the Verrucariaceae (Ascomycota) based on molecular and morphological evidence: recent progress and remaining challenges. Taxon 58, 184208.CrossRefGoogle Scholar
Gueidan, C, Van Do, T and Lu, NT (2014) Phylogeny and taxonomy of Staurothele (Verrucariaceae, lichenized ascomycetes) from the karst of northern Vietnam. Lichenologist 46, 515533.CrossRefGoogle Scholar
Hall, TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 9598.Google Scholar
Harada, H (1991) Thelidium japonicum, a new species of the lichen family Verrucariaceae from Japan. Natural History Research 1, 911.Google Scholar
Harada, H (1992 a) A taxonomic study on the lichen genus Staurothele (Verrucariaceae) in Japan. Natural History Research 2, 3942.Google Scholar
Harada, H (1992 b) Taxonomic notes on the lichen family Verrucariaceae in Japan (I). Five species of Verrucaria previously reported from Japan. Journal of Japanese Botany 67, 218226.Google Scholar
Harada, H (1993) Taxonomic notes on the lichen family Verrucariaceae in Japan (IV). Verrucaria minuscula sp. nov. Hikobia 11, 231233.Google Scholar
Harada, H (1994) Taxonomic notes on the lichen family Verrucariaceae in Japan (V). Verrucaria iwatsukii Harada sp. nov. Hikobia 11, 519522.Google Scholar
Harada, H (1995 a) Taxonomic notes on the lichen family Verrucariaceae in Japan (VII). Verrucaria marinomuralis Harada sp. nov. Natural History Research 3, 111114.Google Scholar
Harada, H (1995 b) Two new species of maritime lichens in the genus Verrucaria (Lichenes, Verrucariaceae) from Japan. Nova Hedwigia 60, 7378.Google Scholar
Harada, H (1995 c) Endocarpon minutum Harada sp. nov. (lichenized Ascomycotina, Verrucariaceae) from western Japan. Bryologist 98, 385388.CrossRefGoogle Scholar
Harada, H (1996 a) Verrucaria igii, a new freshwater species of Verrucariaceae (lichenized Ascomycotina) from Japan. Bryologist 99, 343344.CrossRefGoogle Scholar
Harada, H (1996 b) Taxonomic notes on the lichen family Verrucariaceae in Japan (X). Thelidium radiatum Harada sp. nov. Hikobia 12, 133136.Google Scholar
Harada, H (1996 c) Taxonomic notes on the lichen family Verrucariaceae in Japan (IX). Verrucaria rheitrophila Zsch., new to Japan. Journal of Japanese Botany 71, 317322.Google Scholar
Harada, H (1998) Taxonomic notes on the lichen family Verrucariaceae in Japan (XI). Thelidium pacificum, a new maritime species. Hikobia 12, 289291.Google Scholar
Harada, H (2000) Three new maritime species of Verrucaria (lichenized Ascomycota, Verrucariaceae) from Japan. Bryologist 103, 555562.CrossRefGoogle Scholar
Harada, H (2003) Psoroglaena japonica (lichenized Ascomycota, Verrucariaceae), a new species from Chiba-ken, central Japan, with notes on Psoroglaena. Lichenology 2, 510.Google Scholar
Harada, H (2012 a) Taxonomic study on the freshwater species of Verrucariaceae of Japan (1). Verrucaria praetermissa and V. yoshimurae sp. nov. Lichenology 10, 3341.Google Scholar
Harada, H (2012 b) Taxonomic study on the freshwater species of Verrucariaceae of Japan (2). Genus Verrucaria. Lichenology 10, 97135.Google Scholar
Harada, H (2013 a) Agonimia deguchii (lichenized Ascomycota, Verrucariaceae), a new saxicolous species from central Japan. Hikobia 16, 307310.Google Scholar
Harada, H (2013 b) The lichen genus Thelidium (Verrucariaceae) in Japan. Lichenology 11, 5366.Google Scholar
Harada, H and Wang, L-S (1996) Two new freshwater species of Verrucariaceae from Yunnan, China. Lichenologist 28, 297305.CrossRefGoogle Scholar
Harada, H and Wang, L-S (2006 a) Taxonomic study on the freshwater species of Verrucariaceae (lichenized Ascomycota) of Yunnan, China (2). Genus Staurothele. Lichenology 5, 1322.Google Scholar
Harada, H and Wang, L-S (2006 b) Taxonomic study on the freshwater species of Verrucariaceae (lichenized Ascomycota) of Yunnan, China (3). Genus Thelidium. Lichenology 5, 2330.Google Scholar
Harada, H and Wang, L-S (2008) Taxonomic study on the freshwater species of Verrucariaceae (lichenized Ascomycota) of Yunnan, China (4). Genus Verrucaria. Lichenology 7, 124.Google Scholar
Heiðmarsson, S, Gueidan, C, Miadłlikowska, J and Lutzoni, F (2017) Multi-locus phylogeny supports the placement of Endocarpon pulvinatum within Staurothele s. str. (lichenised ascomycetes, Eurotiomycetes, Verrucariaceae). Phytotaxa 306, 3748.CrossRefGoogle Scholar
Löytynoja, A and Goldman, N (2010) webPRANK: a phylogeny-aware multiple sequence aligner with interactive alignment browser. BMC Bioinformatics 11, 579.CrossRefGoogle ScholarPubMed
Lücking, R, Hodkinson, B and Leavitt, SD (2016) The 2016 classification of lichenized fungi in the Ascomycota and Basidiomycota – approaching one thousand genera. Bryologist 119, 361416.CrossRefGoogle Scholar
Magnusson, AH (1940) Lichens from central Asia I. In Hedin, S (ed.), Reports from the Scientific Expedition to the North-Western Provinces of China Under the Leadership of Dr. Sven Hedin. The Sino-Swedish Expedition 13, XI. Botany, 1. Stockholm: Aktiebolaget Thule.Google Scholar
Malcolm, WM and Galloway, DJ (1997) New Zealand Lichens. Checklist, Key, and Glossary. Wellington: Museum of New Zealand Te Papa Tongarewa.Google Scholar
McCarthy, PM (1991) A new species and new records of Verrucaria Schrader (lichenised Ascomycotina, Verrucariaceae) from New Zealand. New Zealand Journal of Botany 29, 283286.CrossRefGoogle Scholar
McCarthy, PM (1995 a) Notes on Australian Verrucariaceae (lichenised Ascomycotina). 5. Staurothele pallidopora sp. nov. from south-eastern Queensland. Muelleria 8, 275277.CrossRefGoogle Scholar
McCarthy, PM (1995 b) Aquatic species of Verrucaria in eastern Australia. Lichenologist 27, 105126.Google Scholar
McCarthy, PM (2003) Additional lichen records from Australia 51. Some aquatic pyrenolichens from Kosciuszko National Park, Snowy Mountains, New South Wales. Australasian Lichenology 52, 1618.Google Scholar
Miller, MA, Pfeiffer, W and Schwartz, T (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In Proceedings of the Gateway Computing Environments Workshop (GCE), 14 November 2010, New Orleans, Louisiana, pp. 18.10.1109/GCE.2010.5676129CrossRefGoogle Scholar
Ohmura, Y and Kashiwadani, H (2018) Checklist of lichens and allied fungi of Japan. National Museum of Nature and Science Monographs 49, 1140.Google Scholar
Olley, L (2011) A provisional checklist to the lichens of Nepal. [WWW document] URL Scholar
Olley, L and Sharma, LR (2013) A provisional checklist of the lichens of Nepal. Journal of the Department of Plant Resources Nepal 35, 1821.Google Scholar
Orange, A (2000) Verrucaria elaeina, a misunderstood European lichen. Lichenologist 32, 411422.CrossRefGoogle Scholar
Orange, A (2012) Semi-cryptic marine species of Hydropunctaria (Verrucariaceae, lichenized Ascomycota) from north-west Europe. Lichenologist 44, 299320.CrossRefGoogle Scholar
Orange, A (2018) A new species-level taxonomy for Trapelia (Trapeliaceae, Ostropomycetidae) with special reference to Great Britain and the Falkland Islands. Lichenologist 50, 342.CrossRefGoogle Scholar
Orange, A (2020) The Verrucaria aethiobola group (lichenised Ascomycota, Verrucariaceae) in North-west Europe. Phytotaxa 459, 115.CrossRefGoogle Scholar
Pérez-Ortega, S, de los Ríos, A, Crespo, A. and Sancho, LG (2010) Symbiotic lifestyle and phylogenetic relationships of the bionts of Mastodia tesselata (Ascomycota, incertae sedis). American Journal of Botany 97, 738752.CrossRefGoogle Scholar
Pérez-Ortega, S, Miller, KA and de los Ríos, A (2018) Challenging the lichen concept: Turgidosculum ulvae (Verrucariaceae) represents an independent photobiont shift to a multicellular blade-like alga. Lichenologist 50, 341356.CrossRefGoogle Scholar
Prieto, M, Martínez, I, Aragón, G and Otálora, MAG (2010) Phylogenetic study of Catapyrenium s. str. (Verrucariaceae, lichen-forming Ascomycota) and related genus Placidiopsis. Mycologia 102, 291304.CrossRefGoogle ScholarPubMed
Prieto, M, Martínez, I, Aragón, G, Gueidan, C and Lutzoni, F (2012) Molecular phylogeny of Heteroplacidium, Placidium, and related catapyrenioid genera (Verrucariaceae, lichen-forming Ascomycota). American Journal of Botany 99, 2335.CrossRefGoogle Scholar
Pykälä, J, Launis, A and Myllys, L (2018) Verrucaria tenebrosa (Verrucariaceae), a new lichen species from Finland and Norway, and notes on the taxonomy of epiphytic taxa belonging to the V. hydrophila complex. Phytotaxa 361, 211221.CrossRefGoogle Scholar
Rai, H, Nag, P, Khare, R, Upreti, DK and Gupta, RK (2016) Twenty-eight new records of lichenized fungi from Nepal: a signature of undiscovered biodiversity in central Himalaya. Proceedings of the National Academy of Sciences, India, Section B Biologial Sciences 87, 13631376.CrossRefGoogle Scholar
Savić, S and Tibell, L (2009) Taxonomy and species delimitation in Sporodictyon (Verrucariaceae) in Northern Europe and the adjacent Arctic – reconciling molecular and morphological data. Taxon 58, 585605.CrossRefGoogle Scholar
Savić, S and Tibell, L (2012) Polyblastia in Northern Europe and the adjacent Arctic. Symbolae Botanicae Upsalienses 36, 169.Google Scholar
Sharma, LR (1995) Enumeration of the Lichens of Nepal. Nepal Bio-diversity profile project. Kathmandu: Department of National Parks & Wildlife Conservation.Google Scholar
Stamatakis, A (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22, 26882690.CrossRefGoogle ScholarPubMed
Stamatakis, A, Hoover, P and Rougemont, J (2008) A fast bootstrapping algorithm for the RAxML web servers. Systematic Biology 57, 758771.CrossRefGoogle Scholar
Tamura, K, Dudley, J, Nei, M and Kumar, S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 15961599.CrossRefGoogle ScholarPubMed
Thüs, H and Nascimbene, J (2008) Contributions toward a new taxonomy of Central European freshwater species of the lichen genus Thelidium (Verrucariales, Ascomycota). Lichenologist 40, 499521.CrossRefGoogle Scholar
Thüs, H, Muggia, L, Pérez-Ortega, S, Favero-Longo, SE, Joneson, S, O'Brien, H, Nelsen, MP, Duque-Thüs, R, Grube, M, Friedl, T, et al. (2011) Revisiting photobiont diversity in the lichen family Verrucariaceae (Ascomycota). European Journal of Phycology 46, 399415.CrossRefGoogle Scholar
Thüs, H, Orange, A, Gueidan, C, Pykälä, J, Ruberti, C, Lo Schiavo, F and Nascimbene, J (2015) Revision of the Verrucaria elaeomelaena species complex and morphologically similar freshwater lichens (Verrucariaceae, Ascomycota). Phytotaxa 197, 161185.CrossRefGoogle Scholar
Wang-Yang, JR and Lai, MJ (1973) A checklist of the lichens of Taiwan. Taiwania 18, 83104.Google Scholar
White, TJ, Bruns, T, Lee, S and Taylor, J (1990) Amplification and direct sequencing of fungal ribosomal genes for phylogenetics. In Innis, M, Gelfand, D, Sninsky, J and White, T (eds), PCR Protocols: a Guide to Methods and Applications. New York: Academic Press, pp. 315322.Google Scholar
Zahlbruckner, A (1930) Teil 3. Lichens. In Handel-Mazzetti, HRE (ed.), Symbolae Sinicae: Botanische Ergebnisse der Expedition der Akademie der Wissenschaften in Wein nach Südwest-China, 1914–1918. Wien: J. SpringerGoogle Scholar
Zoller, S, Scheidegger, C and Sperisen, C (1999) PCR primers for the amplification of mitochondrial small subunit ribosomal DNA of lichen-forming ascomycetes. Lichenologist 31, 511516.CrossRefGoogle Scholar