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Species identification of bivalve larvae using random amplified polymorphic DNA (RAPD): differentiation between Cerastoderma edule and C. lamarcki

Published online by Cambridge University Press:  04 April 2001

C. André ,
M. Lindegarth ,
P.R. Jonsson  and
P. Sundberg
C. André
Affiliation:
Department of Zoology, Stockholm University, S-106 91 Stockholm, Sweden Present address: Tjärnö Marine Biological Laboratory, S-452 96 Strömstad, Sweden
M. Lindegarth
Affiliation:
Department of Zoology, Stockholm University, S-106 91 Stockholm, Sweden Present address: Marine Ecology Laboratories, University of Sydney, NSW 2006, Australia
P.R. Jonsson
Affiliation:
Department of Zoology, Stockholm University, S-106 91 Stockholm, Sweden Present address: Tjärnö Marine Biological Laboratory, S-452 96 Strömstad, Sweden
P. Sundberg
Affiliation:
Department of Zoology, Göteborg University, Medicinaregatan 18, S-413 90 Göteborg, Sweden
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Abstract

The polymerase chain reaction (PCR) was used to produce species-specific DNA markers (RAPDs) from two sibling cockle species and five other co-occurring intertidal bivalves. Amplification reactions with one single primer readily distinguished larvae and adults of Cerastoderma edule from larvae and adults of C. lamarcki, and from adults of Mya arenaria, Macoma balthica, Scrobicularia plana, Venerupis pulastra and Mytilus edulis. Random amplified polymorphic DNA (RAPD) is suggested as a simple and quick method to determine species identity in taxa that are difficult to identify on the basis of morphological characters alone, such as marine bivalve larvae.

Type
SHORT COMMUNICATION
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 79 , Issue 3 , June 1999 , pp. 563 - 565
Copyright
© 1999 Marine Biological Association of the United Kingdom

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