Hostname: page-component-8448b6f56d-t5pn6 Total loading time: 0 Render date: 2024-04-23T19:09:07.866Z Has data issue: false hasContentIssue false
  • English
  • Français

Metal-Containing Blood Cells of Oysters: Ultrastructure, Histochemistry and X-Ray Microanalysis

Published online by Cambridge University Press:  11 May 2009

Brian J. S. Pirie ,
Stephen G. George ,
David G. Lytton  and
John D. Thomson
Brian J. S. Pirie
Affiliation:
N.E.R.C. Institute of Marine Biochemistry, St Fittick's Road, Aberdeen
Stephen G. George
Affiliation:
N.E.R.C. Institute of Marine Biochemistry, St Fittick's Road, Aberdeen
David G. Lytton
Affiliation:
AB 3RA Department of Pathology, University of Tasmania, Hobart, Tasmania 7001
John D. Thomson
Affiliation:
Australia Tasmanian Fisheries Development Authority, 23 Old Wharf, Hobart, Tasmania 7000, Austlia
Get access Rights & Permissions [Opens in a new window]

Extract

The ultrastructure, histochemistry and X-ray microanalysis of the metal-containing blood cells in three species of oyster, Ostrea edulis, Ostrea angasi and Crassostrea gigas are described. Granular cells containing both copper (up to 400 mM) and zinc (up to 1 M) are present in all three species. In addition,Ostrea edulis has two other granular amoebocyte types which specifically accumulate either copper (400 mM) or zinc (12 M). In all types of amoebocyte, the metals are stored in membrane-limited vesicles. The ultrastructure and histochemical staining characteristics of the vesicular contents vary according to metal composition. Zinc-containing granules appear basophilic after sulphide treatment and display homogeneous electron density; copper/zinc-containing granules are less dense and show varying degrees of crystallinity; copper-containing granules appear crystalline (however, any acidophilic or basophilic reaction is masked by the dark brown precipitate of copper sulphide).

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 64 , Issue 1 , February 1984 , pp. 115 - 123
Copyright
Copyright © Marine Biological Association of the United Kingdom 1984

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Boyce, R. & Herdman, W. A. 1897. On a green leucocytosis in oysters associated with the presence of copper in the leucocytes. Proceedings of the Royal Society, 62, 30–38.Google Scholar
Brooks, R. R. & Rumsby, M. G. 1965. The biogeochemistry of trace element uptake by some New Zealand bivalves. Limnology and Oceanography, 10, 521–527.CrossRefGoogle Scholar
Dacie, J. V. & Lewis, S. M. 1975. Practical Haematology. 629 pp. Edinburgh: Churchill Livingstone.Google Scholar
Davies, T. W. & Morgan, A. J. 1976. The application of x-ray analysis in the transmission electron analytical microscope (T.E.A.M.) to the quantitative bulk analysis of biological microsamples. Journal of Microscopy, 107, 47–54.Google Scholar
Feng, S. Y.Feng, J. S.Burke, C. N. & Khairallah, L. H. 1971. Light and electron microscopy of the leucocytes of Crassostrea virginica (Mollusca: Pelecypoda). Zeitschrift fur Zellforschung und mikroskopische Anatomie, 120, 222–245.Google Scholar
George, S. G. & Frazier, J. M. 1983. Some aspects of the relationship between tolerance to heavy metal pollution and metabolism of Cd, Cu and Zn in oysters. Thalassia jugoslavica. (In the Press.)Google Scholar
George, S. G.Pirie, B. J. S.Cheyne, A. R.Coombs, T. L. & Grant, P. T. 1978. Detoxication of metals by marine bivalves: an ultrastructural study of the compartmentation of copper and zinc in the oyster Ostrea edulis. Marine Biology, 45, 147–156.Google Scholar
Hall, T. A. 1971. The microprobe assay of chemical elements. In Physical Techniques in Biological Research, vol. 1 A. Optical Techniques (ed. G. Oster), pp. 157–275. Academic Press.Google Scholar
Hall, T. A. & Gupta, B. L. 1979. Eds quantitation and application to biology. In Introduction to Analytical Electron Microscopy (ed. J. J. Hren, J. Goldstein and D. Joy), pp. 169–197. Plenum Press.Google Scholar
Midorikawa, O. & Eder, M. 1962. Vergleichende histochemische Untersuchungen über Zink im Darm. Histochemie, 2, 444–446.Google Scholar
Orton, J. H. 1923. Summary of an account of investigations into the cause or causes of the unusual mortality among oysters in English oyster beds during 1920 and 1921.Journal of the Marine Biological Association of the United Kingdom, 13, 1–23.CrossRefGoogle Scholar
Pearse, A. G. E. 1972. Histochemistry, vol. 2. 761–1518 pp. Churchill.Google Scholar
Pringle, B. H.Hissong, D. E.Katz, E. L. & Mulawaka, S. T. 1968. Trace metal accumulation by estuarine molluscs. Journal of the Sanitary Engineering Division, American Society of Civil Engineers, 94, 455–47Google Scholar
Roomans, G. M. & Van Gaal, H. L. M. 1977. Organometallic and organometalloid compounds as standards for microprobe analysis of epoxy resin embedded tissue. Journal of Microscopy, 109, 235–240.CrossRefGoogle ScholarPubMed
Ruddell, C. L. 1971a. Elucidation of the nature and function of the granular oyster amoebocytes through histochemical studies of normal and traumatized oyster tissues. Histochemie, 26, 98–112.CrossRefGoogle Scholar
Ruddell, C. L. 1971b. The fine structure of the granular amoebocytes of the Pacific oyster, Crassostrea gigas. Journal of Invertebrate Pathology, 18, 269–275.Google Scholar
Ruddell, C. L. & Rains, D. W. 1975. The relationship between zinc, copper and the basophils of two crassostreid oysters, C. gigas and C. virginica. Comparative Biochemistry and Physiology, 51A, 585–591.Google Scholar
Ruddell, C. L.Dunlap, T.Okazaki, R. K. & Munn, R. 1978. The effect of selected basic dyes on the blood cells, in particular, the basophils, of the Pacific oyster, Crassostrea gigas. Journal of Invertebrate Pathology, 31, 313–323.Google Scholar
Thrower, S. J. & Eustace, I. J. 1973. Heavy metal accumulation in oysters grown in Tasmanian waters. Food Technology in Australia, 25, 546–553.Google Scholar