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Isolation and partial characterisation of the serum immunoglobulin of the flounder, Platichthys flesus

Published online by Cambridge University Press:  11 May 2009

P.J. Glynn  and
A.L. Pulsford
P.J. Glynn
Affiliation:
Department of Biological Sciences, Polytechnic South West, Plymouth, PL4 8AA
A.L. Pulsford
Affiliation:
Plymouth Marine Laboratory, Citadel Hill, Plymouth, PL1 2PB
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Abstract

Whole flounder serum has been fractionated by gel filtration on Ultrogel AcA22. Pooled material from the first major peak of the elution profile was further fractionated by FPLC ion-exchange chromatography and this yielded one major and three minor peaks. Under both reducing and non-reducing conditions SDS electrophoresis showed that the major peak and two of the minor ones were immunoglobulin (Ig). All three of these Ig populations were tetrameric, with estimated molecular weights of 710 kD. However, approximately 90% of the major Ig species consisted of tetramers which were covalently linked by disulphide bonds, whereas the remaining 10% was composed of dimeric molecules held together by non-covalent interactions. The heavy chains of all three Ig populations had apparent molecular weights of approximately 72 kD but the light chain composition showed considerable heterogeneity. In the major population, five polypep-tides were detected in the light chain region of the gel with apparent molecular weights covering the range 22–28 kD. However, there was no difference in the light chain composition of covalently and non-covalently linked tetramers. The two minor populations differed both from each other and from the major species in respect of their light chain compositions. No evidence was found for a monomeric serum Ig, and alkaline urea electrophoresis failed to demonstrate a J chain.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 70 , Issue 2 , May 1990 , pp. 429 - 440
Copyright
Copyright © Marine Biological Association of the United Kingdom 1990

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