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Genetic characteristics of broodstock and offspring of the seven-band grouper (Hyporthodus septemfasciatus) using fluorescent-AFLP markers

Published online by Cambridge University Press:  14 September 2016

Yongshuang Xiao ,
Shuguang Guan ,
Qinghua Liu ,
Hongjun Liu ,
Daode Yu ,
Daoyuan Ma ,
Shihong Xu ,
Jing Liu ,
Ming Dai  and
Zhizhong Xiao
...Show all authors
Yongshuang Xiao
Affiliation:
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, People's Republic of China Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Shuguang Guan
Affiliation:
Marine Biology Institute of Shandong Province, Qingdao 226104, China
Qinghua Liu
Affiliation:
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, People's Republic of China Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Hongjun Liu
Affiliation:
Marine Biology Institute of Shandong Province, Qingdao 226104, China
Daode Yu
Affiliation:
Marine Biology Institute of Shandong Province, Qingdao 226104, China
Daoyuan Ma
Affiliation:
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, People's Republic of China Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Shihong Xu
Affiliation:
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, People's Republic of China Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Jing Liu
Affiliation:
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, People's Republic of China
Ming Dai
Affiliation:
Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, Guangzhou 510300, People's Republic of China
Zhizhong Xiao*
Affiliation:
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, People's Republic of China Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Jun Li*
Affiliation:
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, People's Republic of China Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
*
Correspondence should be addressed to: J. Li and Z. Xiao, Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, People's Republic of China and Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China email: junli@qdio.ac.cn and xzz@qdio.ac.cn
Correspondence should be addressed to: J. Li and Z. Xiao, Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, People's Republic of China and Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China email: junli@qdio.ac.cn and xzz@qdio.ac.cn
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Abstract

Seven-band grouper (Hyporthodus septemfasciatus) is a commercial rocky reef fish in East Asia that has been regarded as a promising species for aquaculture. To investigate the broodstock contributions to offspring for the sustainability of fry production, 62 individuals of H. septemfasciatus from two broodstocks and one offspring population were analysed using fluorescent-AFLP. A total of 602 bands were amplified and 70.10% of them were polymorphic. The numbers of polymorphic loci were 308 (Pbroodstock I = 55.50%) and 356 (Pbroodstock II = 63.12%) in the two broodstocks, and 294 (Poffspring = 52.88%) in the offspring, respectively. The average values of Shannon diversity index (I) and expected heterozygosity (H) were higher in the broodstock (Ibroodstock I = 0.281, Ibroodstock II = 0.244, Hbroodstock I = 0.185, Hbroodstock II = 0.161) than those in the offspring (Ioffspring = 0.243, Hoffspring = 0.161). AMOVA and FST analyses showed that significant genetic differentiation between broodstock and offspring populations, and limited effective broodstock population size has contributed to the offspring. Both STRUCTURE and Principal Coordinates Analysis (PCoA) also showed the three populations composed of two stocks and most offspring individuals (95.0%) only originated from 44.0% of the individuals of broodstock I, which may have negative effects on sustainable fry production. Therefore, genetic variation between broodstock and offspring should be monitored, and large effective size of broodstock should be employed to ensure the success of commercial breeding programmes. Our data provide a useful genetic basis for future planning of sustainable culture and management of H. septemfasciatus.

Keywords

Serranidaeaquaculturegenetic diversitygenetic structure
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 98 , Issue 2 , March 2018 , pp. 261 - 267
Copyright
Copyright © Marine Biological Association of the United Kingdom 2016 

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