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Synthesis of gold nanoparticles resistant to pH and salt for biomedical applications; functional activity of organic amine

Published online by Cambridge University Press:  18 October 2016

Prem C. Pandey*
Department of Chemistry, Indian Institute of Technology (Banaras Hindu University), Varanasi-221005, India
Govind Pandey
BRD Medical College, Gorakhpur-273013, India
a)Address all correspondence to this author. e-mail:
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The potency of many biomedical applications of gold nanoparticles (AuNPs); i.e., (i) bioimaging, (ii) diagnostic, (iii) therapeutic, (iv) drug carriers, and (v) immunochemical properties; are limited due its sensitivity toward salt and pH allowing variation in nanogeometry during practical applications. Such limitations directed the synthesis of AuNPs having extreme salt and pH resistant ability which has been undertaken in current research program. It has been found that the pH and salt tolerance ability of AuNPs are dependent on the nature of reducing and stabilizing agents. The use of organic amine containing reagents, i.e., polyethylenimine, 3-aminopropyltrimethoxysilane, in the presence of formaldehyde is examined that allows controlled and rapid synthesis of AuNPs having salt and pH tolerance ability. The mechanism justifying these properties of as-made AuNPs are presented herein. These reagents not only allow the synthesis of monometallic nanoparticles (NPs) but also enable the synthesis of bimetallic and trimetallic NPs. The synthesis of Au–Ag/Ag–Au, Pd-Au/Ag@(PdAu) NPs are examined involving the contribution of organic amine.

Invited Feature Paper
Copyright © Materials Research Society 2016 

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Contributing Editor: Lennart Bergström

This paper has been selected as an Invited Feature Paper.



Zhang, L., Gu, F.X., Chan, J.M., Wang, A.Z., Langer, R.S., and Farokhzad, O.C.: Nanoparticles in medicine: Therapeutic applications and developments. Clin. Pharmacol. Ther. 83, 761 (2008).Google Scholar
Davis, M.E., Chen, Z.G., and Shin, D.M.: Nanoparticle therapeutics: An emerging treatment modality for cancer. Nat. Rev. Drug Discovery 7, 771 (2008).CrossRefGoogle ScholarPubMed
Wang, L., O'Donoghue, M.B., and Tan, W.: Nanoparticles for multiplex diagnostics and imaging. Nanomedicine 1, 413 (2006).Google Scholar
Gindy, M.E. and Prud'homme, R.K.: Multifunctional nanoparticles for imaging, delivery and targeting in cancer therapy. Expert Opin. Drug Delivery 6, 865 (2009).Google Scholar
Kumar, M.R., Hellermann, G., Lockeyand, R.F., and Mohapatra, S.S.: Nanoparticle-mediated gene delivery: State of the art. Expert Opin Biol Ther. 4, 1213 (2004).Google Scholar
Ragusa, A., García, I., and Penadés, S.: Nanoparticles as nonviral gene delivery vectors. IEEE Trans Nanobiosci. 6, 319 (2007).Google Scholar
Sokolova, V. and Epple, M.: Inorganic nanoparticles as carriers of nucleic acids into cells. Angew. Chem., Int. Ed. Engl. 47, 1382 (2008).CrossRefGoogle ScholarPubMed
Jin, S., Leach, J.C., and Ye, K.: Nanoparticle-mediated gene delivery. Methods Mol. Biol. 544, 547 (2009).Google Scholar
Chowdhury, E.H. and Akaike, T.: Bio-functional inorganic materials: An attractive branch of gene-based nano-medicine delivery for 21st century. Curr. Gene Ther. 5, 669 (2005).Google Scholar
Ghosh, P.S., Kim, C.K., Han, G., Forbes, N.S., and Rotello, V.M.: Efficient gene delivery vectors by tuning the surface charge density of amino acid-functionalized gold nanoparticles. ACS Nano 2, 2213 (2008).Google Scholar
Pandey, P.C. and Chauhan, D.S.: 3-Glycidoxypropyltrimethoxysilane mediated in situ synthesis of noble metal nanoparticles: Application to hydrogen peroxide sensing. Analyst 137, 376 (2012).CrossRefGoogle ScholarPubMed
Pandey, P.C., Pandey, D., and Pandey, G.: 3 Aminopropyltrimethoxysialne and organic electron donors mediated synthesis of functional gold nanoparticles and their bioanalytical applications. RSC Adv. 4, 60563 (2014).CrossRefGoogle Scholar
Pandey, P.C., Pandey, A.K., and Pandey, G.: Functionalized alkoxysilanes mediated controlled synthesis of noble metal nanoparticles dispersible in aqueous and non-aqueous medium. J. Nanosci. Nanotechnol. 14, 6606 (2014).Google Scholar
Pandey, P.C. and Pandey, G.: Tunable functionality and nanogeometry in tetrahydrofuran hydroperoxide and 3-aminopropyltrimethoxy silane mediated synthesis of gold nano-particles; Functional application in glutathione sensing. J. Mater. Chem. B 2, 3383 (2014).CrossRefGoogle Scholar
Pandey, P.C., Pandey, G., and Narayan, R.J.: Controlled synthesis of polyethylenimine coated gold nanoparticles: Application in glutathione sensing and nucleotide delivery. J. Biomed. Mater. Res., Part B, (2016). doi: 10.1002/jbm.b.33647.Google Scholar
Pandey, P.C. and Pandey, G.: Indian Patent. 4043/DEL/2014.Google Scholar
Pandey, P.C., Pandey, G., Jamal, H., and Pandey, G.: Role of organic carbonyl moiety and 3-aminopropyltrimethoxysilane on the synthesis of gold nanoparticles specific to pH- and salt-tolerance. J. Nanosci. Nanotechnol. 16, 6155 (2016).Google Scholar
Pandey, P.C. and Pandey, G.: One-pot two-step rapid synthesis of 3-aminopropyltrimethoxysilane-mediated highly catalytic Ag@(PdAu) trimetallic nanoparticles. Catal. Sci. Technol. 6, 3911 (2016).Google Scholar
Zhu, H., Pan, Z., Hagaman, E.W., Liang, C., Overbury, S.H., and Dai, S.: Facile one-pot synthesis of gold nanoparticles stabilized with bifunctional amino/siloxy ligands. J. Colloid Interface Sci. 287, 360 (2005).Google Scholar
Pandey, P.C., Upadhyay, S., Tiwari, I., and Sharma, S.: Novel ferrocene encapsulated palladium-linked ormosil based electrocatalytic biosensor; Role of reactive functional group. Electroanalysis 13, 1519 (2001).Google Scholar
Pandey, P.C., Upadhyay, S., Tiwari, I., and Sharma, S.: Functionalized ormosils-based biosensor probing a horseradish peroxidase-catalyzed reaction. J. Electrochem. Soc. 150, H85 (2003).Google Scholar
Pandey, P.C., Upadhyay, S., and Pathak, H.C.: A new glucose biosensor based on sandwich configuration of organically modified sol–gel glass. Electroanalysis 11, 59 (1999).Google Scholar
Pandey, P.C., Upadhyay, S., Pathak, H.C., Tiwari, I., and Tripathi, V.: Studies on glucose biosensors based on nonmediated and mediated electrochemical oxidation of reduced glucose oxidase encapsulated within organically modified sol–gel glasses. Electroanalysis 11, 1251 (1999).Google Scholar
Wight, A.P. and Davis, M.E.: Design and preparation of organic–inorganic hybrid catalysts. Chem. Rev. 102, 3589 (2002).CrossRefGoogle ScholarPubMed
Pandey, P.C., Singh, R., and Pandey, A.K.: Tetrahydrofuran hydroperoxide and 3-aminopropyltrimethoxysilane mediated synthesis of Pd, Pd–Au, Au–Pd nanoparticles: Role of palladium nanoparticles on the redox electrochemistry of ferrocene monocarboxylic acid. Electrochim. Acta 138, 163 (2014).Google Scholar
Pandey, P.C. and Singh, R.: Controlled synthesis of Pd and Pd–Au nanoparticles: Effect of organic amine and silanol groups and morphology and polycrystallinity of nanomaterials. RSC Adv. 5, 10964 (2015).Google Scholar
Pandey, P.C., Singh, R., and Pandey, Y.: Controlled synthesis of functional Ag, Ag–Au/Au–Ag nanoparticles and their Prussian blue nanocomposites for bioanalytical applications. RSC Adv. 5, 49671 (2015).Google Scholar