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Hearing outcome of early postnatal exposure to hypoxia in Sprague–Dawley rats

Published online by Cambridge University Press:  15 April 2014

S J Daniel*
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Montreal Children's Hospital – McGill University Health Centre, Quebec, Canada McGill Auditory Sciences Laboratory, Montreal Children's Hospital – McGill University Health Centre, Quebec, Canada
M McIntosh
Affiliation:
Division of Cardiology, Montreal Children's Hospital – McGill University Health Centre, Quebec, Canada
O V Akinpelu
Affiliation:
McGill Auditory Sciences Laboratory, Montreal Children's Hospital – McGill University Health Centre, Quebec, Canada
C V Rohlicek
Affiliation:
Division of Cardiology, Montreal Children's Hospital – McGill University Health Centre, Quebec, Canada Department of Physiology, McGill University, Montreal, Quebec, Canada
*
Address for correspondence: Dr S J Daniel, Department of Otolaryngology, Head and Neck Surgery, McGill University, Montreal Children's Hospital, B240, 2300 Tupper Avenue, Montreal, Quebec H3H 1P3, Canada Fax: 514-412-4342 E-mail: sam.daniel@mcgill.ca

Abstract

Objective:

To determine the effect of experimentally induced hypoxia, in the first 10 days of life, on physiological hearing in a Sprague–Dawley rat model.

Methods:

A prospective, controlled animal study was carried out using 22 male rat pups. The rats in the hypoxic group (n = 12) were reared in hypoxia for the first 10 days of life, and subsequently reared in normoxia, while those in the control group (n = 10) were reared in normoxia for the duration of the experiment. Hearing was assessed using auditory brainstem response testing at approximately 72 days of age.

Results:

The hypoxia group had higher auditory brainstem response thresholds for all frequencies tested (more pronounced at 16 kHz), compared with controls. Wave I–V inter-peak latencies were more prolonged in the hypoxic rats, while both groups had similar wave I latencies.

Conclusion:

Chronic postnatal hypoxia induced permanent hearing loss in this Sprague–Dawley rat model. Prolonged wave I–V inter-peak latencies suggested functional abnormality in the central auditory pathway.

Type
Main Articles
Copyright
Copyright © JLO (1984) Limited 2014 

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