Hostname: page-component-77c89778f8-vsgnj Total loading time: 0 Render date: 2024-07-18T21:35:46.857Z Has data issue: false hasContentIssue false
  • English
  • Français

Distribution of SP- and CGRP-immunoreactivity in the cat's larynx

Published online by Cambridge University Press:  29 June 2007

Yasumasa Tanaka ,
Yoshikazu Yoshida ,
Minoru Hirano ,
Masatoshi Morimoto  and
Takeshi Kanaseki
Yasumasa Tanaka*
Affiliation:
Department of Otorhinolaryngology and Head and Neck Surgery, School of Medicine, Kurume University, Kurume, Japan
Yoshikazu Yoshida
Affiliation:
Department of Otorhinolaryngology and Head and Neck Surgery, School of Medicine, Kurume University, Kurume, Japan
Minoru Hirano
Affiliation:
Department of Otorhinolaryngology and Head and Neck Surgery, School of Medicine, Kurume University, Kurume, Japan
Masatoshi Morimoto
Affiliation:
Department of Anesthesiology, Saga Medical College, Saga, Japan
Takeshi Kanaseki
Affiliation:
Saga Medical College, Saga, Japan
*
Yasumasa Tanaka, M.D., Department of Otorhinolaryngology and Head and Neck Surgery, School of Medicine, Kurume University, 67, Asahimachi, Kurume 830, Japan Fax: 942-37-1200
Get access Rights & Permissions [Opens in a new window]

Abstract

The distribution of substance P (SP) and calcitonin gene-related peptide (CGRP) immunoreactive (ir) fibres in the cat's larynx was investigated utilizing immunohistochemistry. Many SP- and CGRP-ir fibres with varicosities were found within and below the epithelium and along the basement membrane of the mucosa of all different regions except in the membranous portion of the vocal fold. In the subepithelium, some SP- and CGRP-ir nerve bundles and nerve fibres were recognized around the vessels and glands. In the mucosa, the pattern of distribution and the density of SR-ir fibres were similar to those of CGRP-ir fibres. These reactive fibres were denser in the supraglottic region than in the subglottic region. In the taste bud-like structures, only SP-ir fibres appeared, whereas in the motor endplates, CGRP-reaction was found exclusively. The present findings suggest that the regional distribution of SP- and CGRP-immunoreactivity might be related with sensory and autonomic innervation in the larynx.

Keywords

NeuropeptidesAnimallaboratorycatLarynx
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 107 , Issue 6 , June 1993 , pp. 522 - 526
Copyright
Copyright © JLO (1984) Limited 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Basterra, J., Dilly, P. N., Martorell, M. A. (1989) The autonomic innervation of the human vocal cord: neuropeptides. Laryngoscope 99: 293–296Google Scholar
Cadieux, A., Springall, D. R., Mulderry, P. K., Rodrigo, J., Ghatei, M. R., Terenghi, G., Bloom, S. R., Polak, J. M. (1986) Occurrence, distribution and ontogeny of CGRP-immunoreactivity in the rat lower respiratory tract: effect of capsaicin treatment and surgical denervations. Neuroscience 19: 605–627Google Scholar
Domeij, S., Dahlqvist, A., Forsgren, S. (1991) Studies on colocalization of neuropeptide Y, vasoactive intestinal polypeptide, catecholamine-synthesizing enzymes and acetylecholinesterase in the larynx of the rat. Cell and Tissue Research 263: 495–505Google Scholar
Domeij, S., Carlsoo, B., Dahlqvist, A., Forsgren, S. (1990) Occurrence of mast cells in relation to the distribution of nerve fibers in the rat larynx. Acta Otolaryngologica (Stockholm) 111: 981–989Google Scholar
Domeij, S., Dahlqvist, A., Forsgren, S. (1991) Regional differences in the distribution of nerve fibers showing substance P-and calcitonin gene-related peptide-like immunoreactivity in the rat larynx. Anatomy and Embryology 1: 49–56Google Scholar
Hisa, Y., Sato, F, Fukui, K., Ibata, Y.Mizukoshi, O. (1985) Substance P nerve fibers in the canine larynx by PAP immunohistochemistry. Acta Otolaryngologica (Stockholm) 100: 128–133Google Scholar
Kawazoe, M., Shin, T., Masuko, S. (1990) Distribution of neuropeptide-like immunoreactive fibers in the canine larynx. Otolaryngology—Head and Neck Surgery 103: 957–962Google Scholar
Krekel, J., Weihe, D., Nohr, D., Yanaihara, N., Weber, E. (1990) Distribution of Met-enkephalyl-Ary-Gly-Leu in rat larynx: partial coexistence with vasoactive intestinal polypeptide, peptide histidine isoleucine and neuropeptide Y. Neuroscience Letters 119: 64–67Google Scholar
Lundberg, J. M., Hokfelt, T., Martling, C.-R., Saria, A., Cuello, C. (1984) Substance P-immunoreactive sensory nerves in the lower respiratory tract of various mammals including man. Cell and Tissue Research 235: 251–261Google Scholar
Ninoyu, O., Yamashita, T., Kumazawa, T., Tsubara, A. (1983) Immunohistochemical studies of peptidergic innervation in the field of otolaryngology. Part III: Distribution of peptidergic nerve fibers in the respiratory tract. Practica Otologica (Kyoto) 76: 3229–3240Google Scholar
Shin, T., Wada, S., Maeyama, T. (1987) Substance P immunoreactive nerve fibers of the canine laryngeal mucosa. In Neurolaryngology—Recent Advances. (Hirano, M., Kirchner, J. A., Bless, D. M., eds.), A College Hill Publication, Little, Brown Company, Boston, Toronto, San Diego.Google Scholar
Terenghi, G., Polak, J. M., Rodrigo, J., Mulderry, P. K., Bloom, S. R. (1986) Calcitonin gene-related peptide immunoreactive nerves in the tongue, epiglottis and pharynx of the rat: occurrence, distribution and origin. Brain Research 365: 1–14Google Scholar
Tsuda, K., Shin, T., Masuko, S. (1992) Immunohistochemical study of intralaryngeal ganglia in the cat. Otolaryngology—Head and Neck Surgery 106: 42–46Google Scholar
Uddman, R., Alumets, J., Densert, O., Hakanson, R., Sundler, F. (1978) Occurrence and distribution of VIP nerves in the nasal mucosa and tracheobronchial wall. Acta Otolaryngologica (Stockholm) 86: 443–448Google Scholar