Hostname: page-component-848d4c4894-4rdrl Total loading time: 0 Render date: 2024-07-05T21:07:03.715Z Has data issue: false hasContentIssue false
  • English
  • Français

The behaviour of tympanic membrane perforations in tissue culture: a scanning electron microscopic study

Published online by Cambridge University Press:  29 June 2007

Erling Grønvold Olsen  and
Lars-Eric Stenfors
Erling Grønvold Olsen
Affiliation:
Department of Ophthalmology, University of Tromsö, Tromsö, Norway and the Department of Otolaryngology
Lars-Eric Stenfors*
Affiliation:
Department of Ophthalmology, Central Hospital of Keski-Pohjanmaa, Kokkola, Finland
*
Professor Lars-Eric Stenfors, Department of Otolaryngology, University of Tromsö, N-9038 Tromsö, Norway
Get access Rights & Permissions [Opens in a new window]

Abstract

The effects of keeping rat tympanic membranes with an artificially made pars tensa perforation in tissue culture were observed under a scanning electron microscope. After one day and onwards, spreading and thickening of the keratinizing, outer squamous epithelium (OE) was noted. In addition, ballooning of the innermost cells of the outer epithelium apposing the inner tympanic epithelium (IE) was seen. No appreciable reaction was noted in the connective tissue layer of the drum. The inner tympanic epithelium appeared to be swollen, containing spherical structures in the cytoplasm, especially close to the area of contact with the outer meatal epithelium. No complete cover of the drum defect was seen after 14 days of tissue culture. Hyperplasia and spreading of the keratinizing, outer squamous epithelium of the drum is not sufficient to achieve covering of a drum perforation and complete healing cannot take place unless supported by granulation tissue formation.

Keywords

Tissue cultureTympanic membraneWound healingEar, middle
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 107 , Issue 12 , December 1993 , pp. 1106 - 1109
Copyright
Copyright © JLO (1984) Limited 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Albiin, N.Hellström, S., Salén, B.,Stenfors, L.-E., Wirell, S. (1985) The vascular supply of the rat tympanic membrane. Anatomical Record 212: 17–22CrossRefGoogle ScholarPubMed
Boedts, D. (1978) The tympanic epithelium in normal and pathological conditions. Acta Oto-Rhino-Laryngologica (Belgica) 32: 295–20Google ScholarPubMed
Clawson, J. P., Litton, W. B. (1971) The healing process of tympanic membrane perforations. Transactions of the American Academy of Ophthalmology and Otolaryngology 75: 1302–1312Google ScholarPubMed
Kristensen, S. (1992) Spontaneous healing of traumatic tympanic membrane perforations in man: a century of experience. Journal of Laryngology and Otology 106: 1037–1050CrossRefGoogle Scholar
Mclntire, C.Benitez., J. T. (1970) Spontaneous repair of the tympanic membrane. Histopathological studies in the cat. Annals of Otology, Rhinology and Laryngology 79: 1129–1139CrossRefGoogle Scholar
McMinn, R. M. H., Taylor, M. (1966) The cytology of repair in experimental perforations of the tympanic membrane. British Journal of Surgery 54: 222–232Google Scholar
Reijnen, C. H. J., Kuijpers, W. (1971) The healing pattern of the drum membrane. Acta Otolaryngologica Supplement 287: 7–74Google ScholarPubMed
Shrapnell, H. J. (1832) On the form and structure of the membrana tympani. London Medical Gazette 10: 120–124Google Scholar
Stenfors, L.-E.Carlsöö, B., Salén, B., Winblad, B. (1980) Repair of experimental tympanic membrane perforations. Acta Otolaryngologica 90: 332–341CrossRefGoogle ScholarPubMed
Stenfors, L.-E.Bloom, G. D., Hellström, S. (1984) The tympanic membrane. Acta Otolaryngologica Supplement 414: 28–30Google Scholar
Stenfors, L.-E. (1989) Repair of tympanic membrane perforations using hyaluronic acid: an alternative to myringoplasty. Journal of Laryngology and Otology 103: 39–40Google Scholar
Stenfors, L.-E., Olsen, E. G., Henriksen, A.. (1990) Pars flaccida perforation in tissue culture: morphological study. Journal of Laryngology and Otology 104: 91–96CrossRefGoogle ScholarPubMed
Taylor, M., McMinn, R. M. H. (1965) Healing of experimental perforations of the tympanic membrane. British Journal of Surgery 53: 148–158Google Scholar