Hostname: page-component-77c89778f8-rkxrd Total loading time: 0 Render date: 2024-07-17T07:06:37.647Z Has data issue: false hasContentIssue false
  • English
  • Français

Visceral infection by Porocephalus spp. (Pentastomida) in Neotropical wild mammals

Published online by Cambridge University Press:  02 April 2020

L.A. Gomez-Puerta Open the ORCID record for L.A. Gomez-Puerta [Opens in a new window] ,
L. Baselly ,
M.T. Lopez-Urbina ,
A.E. Gonzalez  and
P. Mayor
L.A. Gomez-Puerta*
Affiliation:
Laboratorio de Epidemiologia y Economía Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Av. Circunvalación 2800, San Borja, Lima 41, Peru
L. Baselly
Affiliation:
Departamento de Cirugia, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho, Botucatu, São Paulo, Brazil
M.T. Lopez-Urbina
Affiliation:
Laboratorio de Epidemiologia y Economía Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Av. Circunvalación 2800, San Borja, Lima 41, Peru
A.E. Gonzalez
Affiliation:
Laboratorio de Epidemiologia y Economía Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Av. Circunvalación 2800, San Borja, Lima 41, Peru
P. Mayor
Affiliation:
Departament de Sanitat i Antomia Animals, Faculty of Veterinary, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain
*
Author for correspondence: L.A. Gomez-Puerta, E-mail: lgomezp@unmsm.edu.pe
Get access Rights & Permissions [Opens in a new window]

Abstract

Larval stages of pentastomids were collected from different organs of small mammals from the Peruvian Amazon. These parasitized mammals included: a western Amazonian oryzomys (Hylaeamys perenensis), an elegant oryzomys (Euryoryzomys nitidus), a lowland paca (Cuniculus paca), two kinkajous (Potos flavus), two silvery woolly monkeys (Lagothrix poeppigii) and a brown-mantled tamarin (Leontocebus fuscicollis). Pentastomids were found in the mesentery and parenchyma of the liver and lungs of these animals. All pentastomids were morphologically identified as nymphs of Porocephalus spp. Only the nymphs collected from select animals (the western Amazonian oryzomys, the elegant oryzomys and the brown-mantled tamarin) were analysed molecularly. Molecular analysis was performed amplifying the mitochondrial cytochrome c oxidase subunit I gene from select nymphs collected from the western Amazonian oryzomys, the elegant oryzomys and the brown-mantled tamarin. The nucleotide sequences exhibited 95.8–97.7% similarity between them. Also, these sequences showed an identity of 95.8–97.9% to Porocephalus crotali (GenBank accession numbers MG559647–MG559655). Molecular analysis indicated the presence of at least two Porocephalus species. These findings represent the first record of Porocephalus in these mammals, thus adding new intermediate hosts for this pentastomid genus. This work represents the first molecular data of Porocephalus in a Neotropical climate.

Keywords

PentastomidaPorocephaluspentastomiasisAmazonwildlife
Type
Short Communication
Information
Journal of Helminthology , Volume 94 , 2020 , e141
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Christoffersen, ML and De Asis, JE (2013) A taxonomic monograph of the recent Pentastomida, with a compilation of their hosts. Zoologische Mededelingen 87, 1–206.Google Scholar
Christoffersen, ML and De Asis, JE (2015) Class Eupentastomida Waloszek, Repetski & Maas, 2006. Vol. 5 in von Vaupel Klein, JC, Charmantier-Daures, M and Schram, FR (Eds) Treatise on zoology – anatomy, taxonomy, biology. The crustacea. Leiden, Netherlands, Brill.Google Scholar
Esslinger, JH (1962) Development of Porocephalus crotali (Humboldt, 1808) (Pentastomida) in experimental intermediate hosts. Journal of Parasitology 48, 452–456.CrossRefGoogle Scholar
Folmer, O, Black, M, Hoeh, W, Lutz, R and Vrijenhoek, R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3, 294–299.Google ScholarPubMed
Fox, JG, Diaz, JR and Barth, RA (1972) Nymphal Porocephalus clavatus in the brain of a squirrel monkey, Saimiri sciureus. Laboratory Animal Science 22, 908–910.Google ScholarPubMed
Gárate, I, Naupay, A, Suyo, B, Colquichagua, H, Rodríguez, E and Yarlequé, A (2007) Identificación de Porocephalus stilessi (PENTASTOMIDA) en la serpiente peruana Lachesis muta. Revista de Investigaciones Veterinarias del Perú 18, 89–93.Google Scholar
Gomez-Puerta, LA, Lopez-Urbina, MT and Gonzalez, AE (2011) Presence of Porocephalus clavatus (Arthropoda: Porocephalidae) in Peruvian Boidae snakes. Veterinary Parasitology 181, 379–381.CrossRefGoogle ScholarPubMed
Gomez-Puerta, LA, Alarcon, V, Pacheco, J, Franco, F, Lopez-Urbina, MT and Gonzalez, AE (2016) Molecular and morphological evidence of Taenia omissa in pumas (Puma concolor) in the Peruvian Highlands. Revista Brasileira de Parasitologia Veterinária 25, 368–373.CrossRefGoogle ScholarPubMed
Hall, JE, Haines, DE and Frederickson, RG (1985) Pentastomid nymph from the brain of a squirrel monkey (Saimiri sciureus). I. Morphology of the nymph. Journal of Medical Primatology 14, 195–208.Google ScholarPubMed
Hu, M, Chilton, NB, Zhu, X and Gasser, RB (2002) Single-strand conformation polymorphism-based analysis of mitochondrial cytochrome c oxidase subunit 1 reveals significant substructuring in hookworm populations. Electrophoresis 23, 27–34.3.0.CO;2-7>CrossRefGoogle ScholarPubMed
Kelehear, C, Spratt, DM, Dubey, S, Brown, GP and Shine, R (2011) Using combined morphological, allometric and molecular approaches to identify species of the genus Raillietiella (Pentastomida). PLoS One 6, e24936.CrossRefGoogle Scholar
Kelehear, C, Spratt, DM, O'Meally, D and Shine, R (2014) Pentastomids of wild snakes in the Australian tropics. International Journal for Parasitology: Parasites and Wildlife 3, 20–31.Google ScholarPubMed
Martinez, FA (1982) Ninfas de Porocephalus crotali Humboldt, 1808 (Arachnida; Pentastomida) en edentatos de Argentina. Boletin Chileno de Parasitologia 37, 74–75.Google Scholar
Miller, MA, Kinsella, JM, Snow, RW, Hayes, MM, Falk, BG, Reed, RN, Mazzotti, FJ, Guyer, C and Romagosa, CM (2018) Parasite spillover: indirect effects of invasive Burmese pythons. Ecology and Evolution 8, 830–840.CrossRefGoogle ScholarPubMed
Pereira, WL, Benigno, RN and Silva, KS (2010) Fatal infections in a captive Pithecia irrorata (primate) by Porocephalus sp. (Pentastomida). Veterinary Parasitology 173, 358–361.CrossRefGoogle Scholar
Rego, AA (1980) Pentastomídeos de mamíferos da coleçăo helmintológica do Instituto Oswaldo Cruz. Revista Brasileira de Biologia 40, 783–791.Google Scholar
Riley, J (1986) The biology of pentastomids. Advances in Parasitology 25, 45–128.CrossRefGoogle ScholarPubMed
Riley, J and Self, JT (1979) On the systematics of the pentastomid genus Porocephalus (Humboldt, 1811) with descriptions of two new species. Systematic Parasitology 1, 25–42.CrossRefGoogle Scholar
Tantaleán, M and Gozalo, A (1985) Parásitos de Bothrops atrox (Viperidae) de la Amazonía Peruana. Revista de la Asociación de Médicos Veterinarios Especialistas en Animales Pequeño 20, 11–12.Google Scholar
Vargas, M (1970) A contribution to the morphology of the eggs and nymphal stages of Porocephalus stilesi Sambon, 1910 and Porocephalus clavatus (Wyman, 1847) Sambon, 1910 (Pentastomida). Revista de Biología Tropical 17, 27–89.Google Scholar
Zhang, G, Chen, J, Yang, Y, Liu, N, Jiang, W, Gu, S, Wang, X and Wang, Z (2014) Utility of DNA barcoding in distinguishing species of the family Taeniidae. Journal of Parasitology 100, 542–546.CrossRefGoogle ScholarPubMed