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Vaccination of mice with a 30 kDa Schistosoma antigen with and without human adjuvant induces high protection against S. mansoni infection

Published online by Cambridge University Press:  12 April 2024

A.M. Attallah ,
M.M. Abdel Aziz ,
A.T. Abbas ,
K.A. Elbakry  and
M.M. El-Sharabasy
A.M. Attallah*
Affiliation:
Biotechnology Research Center, PO Box 14, 23 July Street, Industrial Zone, New Damietta City 34517, Egypt
M.M. Abdel Aziz
Affiliation:
Gastro-enterology Center, Mansoura University, Mansoura 35111, Egypt
A.T. Abbas
Affiliation:
Gastro-enterology Center, Mansoura University, Mansoura 35111, Egypt
K.A. Elbakry
Affiliation:
Gastro-enterology Center, Mansoura University, Mansoura 35111, Egypt Faculty of Science – Damietta, Mansoura University, New Damietta 34517, Egypt
M.M. El-Sharabasy
Affiliation:
Faculty of Science – Damietta, Mansoura University, New Damietta 34517, Egypt
*
*Fax: +20 57 401889, Email amattallah@hotmail.com
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Abstract

A 30 kDa antigen was characterized as a hydrophobic polypeptide containing 16 amino acids and evaluated as a potential candidate vaccine against infection by Schistosoma mansoni. CD1 albino mice immunized at 0, 14, and 21 days with 25 or 50 μg of the 30 kDa antigen per mouse with and without alum developed high levels of IgG antibodies (predominantly IgG2a and IgG2b isotypes). When immunized mice were infected with 200 S. mansoni cercariae, the highest protection levels (61% and 65% reduction in worm burden in two separate experiments) were obtained using the 50-μg antigen without alum adjuvant. The granuloma size decreased to 10%, a non-significant level in mice immunized using alum adjuvant. The results demonstrate the ability of the 30 kDa antigen with and without alum adjuvant to protect mice against S. mansoni infection.

Type
Review Article
Information
Journal of Helminthology , Volume 78 , Issue 3 , September 2004 , pp. 189 - 194
Copyright
Copyright © Cambridge University Press 2004

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