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Use of an aceto-carmine procedure to examine the excysted metacercariae of Echinostoma caproni and E. trivolvis

Published online by Cambridge University Press:  05 June 2009

B. Fried  and
P. M. Manger Jr
B. Fried
Affiliation:
Department of Biology, Lafayette College, Easton, Pennsylvania 18042, USA
P. M. Manger Jr
Affiliation:
Department of Biology, Lafayette College, Easton, Pennsylvania 18042, USA
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Abstract

A simple aceto-carmine procedure was developed to relax, fix, stain, and clear excystcd metacercariae of Echinostoma caproni and E. trivolvis. This procedure allowed for morphologic details on whole metacercariae comparable to those seen with more elaborate staining procedures. Differences in dimensions and staining intensities between the two species of metacercariae are described.

Keywords

techniquesaceto-carminestainingexcysted metacercariaeTrematodaEchinosToma caproniEchinostoma trivolvis
Type
Research Note
Information
Journal of Helminthology , Volume 66 , Issue 3 , September 1992 , pp. 238 - 240
Copyright
Copyright © Cambridge University Press 1992

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References

REFERENCES

Fried, B. & Emili, S. (1988) Excystation in vitro of Echinostoma liei and E. revolutum (Trematoda) metacercariae. Journal of Parasitology 74, 98–102.CrossRefGoogle Scholar
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