Hostname: page-component-848d4c4894-mwx4w Total loading time: 0 Render date: 2024-06-17T00:14:15.349Z Has data issue: false hasContentIssue false
  • English
  • Français

Ultrastructural study of Trichinella spiralis with emphasis on adult male reproductive organs

Published online by Cambridge University Press:  05 June 2009

Y. Takahashi ,
C. Goto  and
K. K. Kita
Y. Takahashi
Affiliation:
Department of Parasitology, Gifu University School of Medicine, 40 Tsukasa, Gifu, 500, Japan
C. Goto
Affiliation:
Department of Parasitology, Gifu University School of Medicine, 40 Tsukasa, Gifu, 500, Japan
K. K. Kita
Affiliation:
Department of Parasitology, Gifu University School of Medicine, 40 Tsukasa, Gifu, 500, Japan
Get access Rights & Permissions [Opens in a new window]

Abstract

The ultrastructure of the reproductive system of adult male Trichinella spiralis has been examined, particularly to assist in recent advances such as the localization of target antigens of protective immunity and the mode of immune attack. The male reproductive system consists of a single tube with a hairpin-like bend, composed of a basal lamina, epithelial cells, rachis, circumferential and constrictor muscles, and germinal cells. The organs were surrounded by basal lamina and haemolymph. Germinal cells in different stages of maturation were found on the wall of the testis along its entire length. As the maturation of germinal cells proceeded, the cells moved towards the lumen of the testis. The germinal cells had a row of vesicles (cup-shaped structures) at the cell periphery. The mature sperm, lacking flagella and an acrosome, were stored in the seminal vesicle. The cytoplasm of the epithelial cells of the seminal vesicle and ejaculatory duct was filled with distended rough endoplasmic reticulum (rER) and exocrine granules which appeared homogenous and of medium electron density. The granules appeared to discharge to the lumen.

Type
Research Papers
Information
Journal of Helminthology , Volume 68 , Issue 4 , December 1994 , pp. 353 - 358
Copyright
Copyright © Cambridge University Press 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Chitwood, B.G. (1930) The structure of the oesophagus in the Trichuroidea. Journal of Parasitology 17, 35–42.CrossRefGoogle Scholar
Despommier, D.D. & Campbell, W.C. (1983) Synopsis of Morphology, in Campbell, W.C. (Ed.) Trichinella and Trichinosis. New York and London, Plenum Press.Google Scholar
Gualzata, M., Weiss, N. & Heusse, C.H.H. (1986) Dipetalonema viteae: phosphorylcholine and non-phosphorylcholine anti-genie determinants in infective larvae and adult worms. Experimental Parasitology 61, 95–102.CrossRefGoogle ScholarPubMed
Gualzata, M., Rudin, W., Weiss, N. & Heusse, C.H.H. (1988) The cross-reactive immune response between infective larvae and adult worms of Acanthocheilonema (Dipetalonema) viteae is dominated by phosphorylcholine. Parasite Immunology 10, 481–492.CrossRefGoogle ScholarPubMed
Lee, D.L. (1971) The structure and development of the spermatozoon of Heterakis gallinarum (Nematoda). Journal of Zoology 164, 181–187.CrossRefGoogle Scholar
Lee, D.L. & Anya, A.O. (1967) The structure and development of the spermatozoon of Aspiculuris tetraptera (Nematoda). Journal of Cell Sciences 2, 537–544.CrossRefGoogle ScholarPubMed
Martin, J. & Lee, D.L. (1980) Observations on the structure of the male reproductive system and spermatogenesis of Ne-matodirus battus. Parasitology 81, 579–586.CrossRefGoogle ScholarPubMed
McLaren, D.J. (1973) The structure and development of the spermatozoon of Dipetalonema viteae (Nematoda: Filariodea). Parasitology 66, 447–463.CrossRefGoogle Scholar
Silberstein, D.S. & Despommier, D.D. (1985) Effects on Trichinella spiralis of host response to purified antigens. Science 227, 948–950.CrossRefGoogle ScholarPubMed
Slomianny, C., Herbaut, C., Vernes, A. & Bigut, J. (1981) Etude ultrastructurale de la spermatogenese chez Trichinella spiralis Owen 1835. Zeitschrift für Parasitenkunde 64, 207–215.CrossRefGoogle Scholar
Takahashi, Y., Uno, T., Mizuno, N., Suzuki, H., Tokuda, C. & Araki, T. (1989) Hematoxylin and eosin staining profile of adult worms of Trichinella spiralis. Japanese Journal of Parasitology 38, 285–289.Google Scholar
Takahashi, Y., Homan, W. & Pak Leong Lim, P.L. (1993) Ultrastructural localization of the phosphorylcholine-associated antigen in Trichinella spiralis. Journal of Parasitology 79, 604–609.CrossRefGoogle ScholarPubMed
Takahashi, Y., Mizuno, N., Araki, T., Okuda, H.. & Nakashima, T.. (1994) Immunocytochemical localization of antigens in adult worms of Trichinella spiralis recognized by Fischer rats. Parasitology Research, 80, 291–296.CrossRefGoogle ScholarPubMed
Uno, T., Mizuno, N., Suzuki, H., Takahashi, Y.. & Araki, T.. (1993) Effects of pepsin-HCl digestion on the infectivity of Trichinella spiralis muscle larvae. Japanese Journal of Parasitology 42, 128–129.Google Scholar
Ward, S., Hogen, E. & Nelson, G.A. (1983) The initiation of spermiogenesis in the nematode Caenorhabditis elegans. Developmental Biology 98, 70–79.CrossRefGoogle ScholarPubMed
Wright, E.J. & Sommerville, R.I. (1985) Structure and development of the spermatozoon of the parasitic nematode, Ne-matospiroides dubius. Parasitology 90, 179–192.CrossRefGoogle Scholar
Wu, L.Y. (1955) Studies on Trichinella spiralis I. male and female reproductive systems. Journal of Parasitology 41, 40–47.CrossRefGoogle Scholar