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Steinernema pwaniensis n. sp., a new entomopathogenic nematode (Nematoda: Steinernematidae) from Tanzania

Published online by Cambridge University Press:  14 January 2016

V. Půža ,
J. Nermut ,
Z. Mráček ,
S. Gengler  and
S. Haukeland
V. Půža*
Affiliation:
Laboratory of Entomopathogenic Nematodes, Institute of Entomology, Biology Centre, Czech Academy of Sciences, Branišovská 31, 370 05, České Budějovice, The Czech Republic
J. Nermut
Affiliation:
Laboratory of Entomopathogenic Nematodes, Institute of Entomology, Biology Centre, Czech Academy of Sciences, Branišovská 31, 370 05, České Budějovice, The Czech Republic
Z. Mráček
Affiliation:
Laboratory of Entomopathogenic Nematodes, Institute of Entomology, Biology Centre, Czech Academy of Sciences, Branišovská 31, 370 05, České Budějovice, The Czech Republic
S. Gengler
Affiliation:
Veterinary and Agrochemical Research Centre (VAR), Brussels, Belgium
S. Haukeland
Affiliation:
International Centre for Insect Physiology and Ecology (ICIPE), Nairobi, Kenya
*
*E-mail: vpuza@seznam.cz
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Abstract

A new species of entomopathogenic nematode, Steinernema pwaniensis n. sp., belonging to the glaseri group, is described from Tanzania. The infective juveniles of S. pwaniensis n. sp. are characterized by a body length of 978 μm (808–1131), distance from anterior end to excretory pore of 86 μm (80–95) and a tail length of 87 μm (75–95). The ratios a, c and E% of S. pwaniensis n. sp. are substantially lower than those of all other African ‘glaseri’ group members. The first-generation males of S. pwaniensis n. sp. can be distinguished by a large spicule length of 92 μm (80–97) and by the absence of the caudal mucron, while second-generation males possess a short spine-like mucron. First-generation females have a peg-like tail tip bearing three spine-like projections. Second-generation females can be recognized by a slightly protruding vulva and well-developed post-anal swelling. The new species is further characterized by sequences of the internal transcribed spacer (ITS) and partial 28S regions of the ribosomal DNA. Phylogenetic analyses show that S. pwaniensis n. sp. forms a strongly supported monophyletic clade with two other East African species, S. ethiopiense and S. karii.

Type
Research Papers
Information
Journal of Helminthology , Volume 91 , Issue 1 , January 2017 , pp. 20 - 34
Copyright
Copyright © Cambridge University Press 2016 

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