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Serodiagnostic potential of chemically synthesized glycosphingolipid antigens in an enzyme-linked immunosorbent assay for alveolar echinococcosis

Published online by Cambridge University Press:  12 April 2024

K. Yamano ,
N. Hada ,
T. Yamamura ,
T. Takeda ,
H. Honma  and
Y. Sawada
K. Yamano*
Affiliation:
Department of Biological Science, Hokkaido Institute of Public Health, North 19, West 12, Kitaku, Sapporo 060-0819, Japan
N. Hada
Affiliation:
Kyoritsu University of Pharmacy, Tokyo, Japan
T. Yamamura
Affiliation:
Kyoritsu University of Pharmacy, Tokyo, Japan
T. Takeda
Affiliation:
Kyoritsu University of Pharmacy, Tokyo, Japan
H. Honma
Affiliation:
Department of Biological Science, Hokkaido Institute of Public Health, North 19, West 12, Kitaku, Sapporo 060-0819, Japan
Y. Sawada
Affiliation:
Department of Biological Science, Hokkaido Institute of Public Health, North 19, West 12, Kitaku, Sapporo 060-0819, Japan
*
*Corresponding author: Fax: 81 11 736 9476, Email: yamano@iph.pref.hokkaido.jp
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Abstract

In the serodiagnosis of alveolar echinococcosis, the detection of specific reactions against not only protein but also carbohydrate antigen is useful and both antigens supplement each other. Though recombinant protein antigens have recently advanced, the preparation of carbohydrate antigen still depends on extraction from crude antigens. In the latter case, it is not conventional to obtain carbohydrate antigen as a single component for examination and research. Therefore, chemically synthesized carbohydrate antigens were prepared for serodiagnosis by the enzyme-linked immunosorbent assay (ELISA). Four antigens with the structure of glycosphingolipids from Echinococcus multilocularis were examined and one antigen, Galβ1-6(Fucα1-3)Galβ1-6Galβ1-ceramide, was found to show significant serodiagnostic potential in differentiating alveolar from cystic echinococcosis.

Type
Research Article
Information
Journal of Helminthology , Volume 80 , Issue 4 , December 2006 , pp. 387 - 391
Copyright
Copyright © Cambridge University Press 2006

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