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Presynaptic and postsynaptic regulation of muscle contractions in the ascarid nematode Ascaris suum: a target for drug action

Published online by Cambridge University Press:  27 November 2015

S.M. Trailović ,
Z. Zurovac ,
S. Gruborović ,
D.S. Marjanović  and
J. Nedeljković-Trailović
S.M. Trailović*
Affiliation:
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Belgrade, Bulevar oslobodenja 18, 11000Belgrade, Serbia
Z. Zurovac
Affiliation:
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Belgrade, Bulevar oslobodenja 18, 11000Belgrade, Serbia
S. Gruborović
Affiliation:
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Belgrade, Bulevar oslobodenja 18, 11000Belgrade, Serbia
D.S. Marjanović
Affiliation:
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Belgrade, Bulevar oslobodenja 18, 11000Belgrade, Serbia
J. Nedeljković-Trailović
Affiliation:
Department of Nutrition and Botany, Faculty of Veterinary Medicine, University of Belgrade, Bulevar oslobodenja 18, 11000Belgrade, Serbia
*
*Fax: +381113066245 E-mail: sasa@vet.bg.ac.rs
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Abstract

The aim of this study was to determine the role in contractions of postsynaptic nicotinic acetylcholine (nACh) and γ-aminobutyric acid (GABA) receptors, in the bag region of Ascaris suum muscle cells, as well as the role of synaptic receptors between interneurons and motor neurons in the dorsal and ventral nerve cord. We have measured the isometric contractions of isolated segments of A. suum, with or without the nerve cord (dorsal or ventral). Contractions were caused by increasing concentrations of ACh or by electrical field stimulation (EFS). Based on our results, the presence of the nerve cord is essential for the contractile effects of ACh. The EC50 value of ACh for innervated muscle strips was 10.88 μm. Unlike intact (innervated) preparations, there was no contraction of the muscle flaps when the nerve cord was mechanically removed. Furthermore, continuous EFS produced stable contractions of innervated muscle strips, but they are not sensitive to mecamylamine (100 μm). However, GABA (30 μm) significantly inhibited the EFS-induced contractions. EFS with the same characteristics did not cause muscle contractions of denervated muscle strips, but EFS with a wider pulse induced the increasing of tone and irregular contractions. These contractions were completely insensitive to the effect of GABA. The EC50 for ACh did not differ between the dorsal and ventral segments (9.83 μm and 9.45 μm), while GABA exhibited features of competitive and non-competitive antagonists, regardless of whether it acted on the dorsal or ventral segments of A. suum. It is obvious that drugs will be more effective if they act on both the synaptic and extrasynaptic nACh and GABA receptors.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 6 , November 2016 , pp. 698 - 705
Copyright
Copyright © Cambridge University Press 2015 

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References

del Castillo, J., Rivera, A., Solórzano, S. & Serrato, J. (1989) Some aspects of the neuromuscular system of Ascaris . Quarterly Journal of Experimental Physiology 74, 1071–1087.CrossRefGoogle ScholarPubMed
Halton, W.D., Behnke, M.J. & Marshall, I. (2001) Practical exercises in parasitology. pp. 211–218. Cambridge, UK, Cambridge University Press.Google Scholar
Harris, J.E. & Crofton, H.D. (1957) Structure and function in the nematodes: internal pressure and cuticular structure in Ascaris . Journal of Experimental Biology 34, 116–130.Google Scholar
Holden-Dye, L. & Walker, R.J. (2007) Anthelmintic drugs. WormBook 2, 1–13.Google Scholar
Holden-Dye, L., Krogsgaard-Larsen, P., Nielsen, L. & Walker, R.J. (1989) GABA receptors on the somatic muscle cells of the parasitic nematode, Ascaris suum: stereoselectivity indicates similarity to a GABAA-type agonist recognition site. British Journal of Pharmacology 98, 841–850.CrossRefGoogle ScholarPubMed
Katzung, B.G., Masters, S.B. & Trevor, A.J. (2012) Basic and clinical pharmacology. New York, USA, McGraw-Hill.Google Scholar
Martin, R.J. (1982) Electrophysiological effects of piperazine and diethylcarbamazine on Ascaris suum somatic muscle. British Journal of Pharmacology 77, 255–265.Google Scholar
Martin, R.J., Purcell, J., Day, T. & Robertson, A.P. (2003) Neurotransmiters. pp. 359–384 in Marr, J.J. & Nilsen, T.W. (Eds) Molecular and medical parasitology. London, UK, Academic Press.CrossRefGoogle Scholar
Puttachary, S., Trailovic, S.M., Robertson, A.P., Thompson, D.P., Woods, D.J. & Martin, R.J. (2013) Derquantel and abamectin: effects and interactions on isolated tissues of Ascaris suum . Molecular and Biochemical Parasitology 188, 79–86.Google Scholar
Stretton, A.O., Fishpool, R.M., Southgate, E., Donmoyer, J.E., Walrond, J.P., Moses, J.E.R. & Kass, I.S. (1978) Structure and physiological activity of the motorneurons of the nematode Ascaris . Proceedings of the National Academy of Sciences, USA 75, 3493–3497.Google Scholar
Trailović, S.M. & Varagić, V.M. (2007) The effect of ivermectin on convulsions in rats produced by lidocaine and strychnine. Veterinary Research Communications 31, 863–872.Google Scholar
Trailović, S.M., Clark, C.L., Robertson, A.P. & Martin, R.J. (2005) Brief application of AF2 produces long lasting potentiation of nAChR responses in Ascaris suum . Molecular and Biochemical Parasitology 139, 51–64.Google Scholar
Trailović, S.M., Robertson, A.P. & Nedeljković-Trailović, J. (2009) Inhibitory effect of eugenol on rat ileal motility in vitro . Acta Veterinaria (Beograd) 59, 123–131.Google Scholar
Trailović, S.M., Ivanovic, S.R. & Varagić, V.M. (2011) Ivermectin effects on motor coordination and contractions of isolated rat diaphragm. Research in Veterinary Science 91, 426–433.Google Scholar
Trailović, S.M., Marjanović, D.S., Nedeljković Trailović, J., Robertson, A.P. & Martin, R.J. (2015) Interaction of carvacrol with the Ascaris suum nicotinic acetylcholine receptors and gamma-aminobutyric acid receptors, potential mechanism of antinematodal action. Parasitology Research 114, 3059–3068.Google Scholar
Wolstenholme, A.J. (2011) Ion channels and receptor as targets for the control of parasitic nematodes. International Journal for Parasitology – Drugs and Drug Resistance 1, 2–13.Google Scholar