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Pathological Changes and Vitamin B12 Metabolism in Sheep Parasitised by Haemonchus contortus, Ostertagia spp. and Trichostrongylus colubriformis

Published online by Cambridge University Press:  18 November 2009

M. R. Gardiner
M. R. Gardiner
Affiliation:
Animal Health Laboratory, Department of Agriculture, W.A.
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Extract

Groups of helminth-free weaner sheep were equilibrated on a ration containing almost no cobalt for 3 months. Cobalt supplementation was given orally thrice weekly to provide the minimum requirement for sheep. Infective larvae of Haemonchus contortus, Trichostrongylus colubriformis and Ostertagia spp. were given at two dosage levels to each of three groups, respectively, for 18 days, followed after a lapse of 30 days by small daily dosings of a mixed suspension of these helminths. Serum vitamin B12 was determined for a month before parasitization and for up to 12 weeks thereafter for every sheep. Sheep parasitized with higher numbers of T. colubriformis and Ostertagia spp. were significantly affected with loss of weight and in three cases, death, although there were no changes in the serum levels of vitamin B12 which remained fairly constantly at the lower end of the normal range during most of the parasitic stage. Sheep dying from these infestations showed terminal rises of serum vitamin B12 whilst those surviving showed falls in the last few weeks of the trial associated with significant decreases in liver cobalt levels. Morphological changes of some severity were found in the abomasum and intestines of the sheep receiving T. colubriformis and Ostertagia spp. and appeared to limit the uptake of vitamin B12. It is concluded that mild to moderate infestations of intestinal trichostrongyles and to a lesser degree Ostertagia spp. will interfere with the absorption of vitamin B12 and that these helminths may precipitate cobalt deficiency syndromes if cobalt is at or near the critical level in the feed.

Type
Research Papers
Information
Journal of Helminthology , Volume 40 , Issue 1-2 , June 1966 , pp. 63 - 76
Copyright
Copyright © Cambridge University Press 1966

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References

Gallagher, C. G., 1903.—“Studies on trichostrongylosis of sheep. Plasma volume, haemoglobin concentration, and blood cell count”. Aust. J. agric. Res., 14, 349–363.Google Scholar
Gardiner, M. R., 1905.—“Mineral metabolism in sheep lupinosis I. Iron and cobalt.” J. comp. Path., 75, 397–408.CrossRefGoogle Scholar
Gawthorne, J. M., 1904.—“The influence of cobalt intake on the concentration of vitamin B12 in sheep serum”. Thesis, Univ. of West. Austral., Perth.Google Scholar
Gordon, H. McL., 1953.—“The epidemiology of helminthosis in sheep in winter-rainfall regions of Australia. I. Preliminary observations”. Austral. vet. J., 29, 337–348.CrossRefGoogle Scholar
Gordon, H. McL., 1958.—“The effect of worm parasites on the productivity of sheep”. Proc. Aust. Soc. Ani. Prod., 2, 59–68.Google Scholar
Hutner, S. H., Bach, M. K. and Ross, G. I. M., 1956.—“A sugar-containing basal medium for vitamin B12 assay with Euglena; application to body fluids”. J. Protoz. 3, 101–112.CrossRefGoogle Scholar
Jacobson, E. D., Prior, J. T. and Faloon, W. W., 1960.—“Malabsorptive syndrome induced by neomycin: Morphologic alterations in the jejunal mucosa”. J. lab. clin. Med., 56, 245–250.Google ScholarPubMed
Kato, N. and Murakami, S., 1959.—“The effect of experimental hepatic necrosis on the vitamin B12 content in serum and liver”. J. lab. clin. Med, 54, 365–380.Google ScholarPubMed
Layrisse, M., 1960.—“The metabolism of vitamin B12 and folic acid in patients with Necalor americanus”. Rev. Venez. sanid. y Asist. Soc., 25, 244–253.Google Scholar
Marston, H. R., Allen, S. H. and Smith, R. M., 1901.—“Primary metabolic defect supervening on vitamin B12 deficiency in the sheep”. Nature, Lond., 190, 1085–1087.Google Scholar
McNaught, K. J., 1948.—“Cobalt, copper and iron in the liver in relation to cobalt deficiency ailment”. N.Z.J. Sci. Tech., 30A, 26–43.Google Scholar
Mollin, D. L. and Ross, G. I. M., 1953.—“Serum vitamin B12 concentrations of patients with megaloblastic anaemia after treatment with vitamin B12, folic and/or folinic acid”. Brit. med. J., Sept. 19, 640–645.CrossRefGoogle Scholar
Pitney, W. R. and Beard, M. F., 1955.—“Vitamin B12 deficiency following total gastrectomy”. Ann. int. Med., 95, 591–593.Google Scholar
Richard, R. M., Shumard, R. F., Pope, A. L., Phillips, P. H., Herrick, C. A. and Bohstedt, G., 1954.—“The effect of certain mineral supplements on lambs infected with the stomach worm (Haemonchus contortus)”. J. ani. Sci., 13, 694–705.CrossRefGoogle Scholar
Sheehy, T. W., Meroney, W. H., Cox, R. S. and Soler, J. E., 1962.—“Hookworm disease and malabsorption”. Gastroenterology, 42, 148–156.CrossRefGoogle ScholarPubMed
Stewart, D. F., 1953.—“Studies on resistance of sheep to infestation with Haemonchus contortus and Trichostrongylus spp. and on the immunological reactions of sheep exposed to infestation. V. The nature of the ‘self cure’ phenomenon”. Aust. J. agric. Res., 4, 100–117.CrossRefGoogle Scholar
Stewart, D. F., 1955.—“‘Self-cure’ in nematode infestations of sheep”. Nature, Lond., 176, 1273–1274.CrossRefGoogle ScholarPubMed
Symons, L. E. A. and Fairbairn, D., 1963.—“Biochemical pathology of the rat jejunem parasitized by the nematode Nippostrongyltis brasiliensis”. Exp. Parasit., 13, 284–304.CrossRefGoogle Scholar
Threlkeld, W. L., Price, N. O. and Linkous, W. N., 1956.—“An observation on the relationship of cobalt deficiency to internal parasites of sheep”. Am. J. vet. Res., 17, 246–251.Google Scholar
Underwood, E. J., 1962.—Trace Elements in Human and Animal Nutrition, 2nd Ed., Academic Press, N.Y. p.137.Google Scholar