Hostname: page-component-848d4c4894-r5zm4 Total loading time: 0 Render date: 2024-07-04T19:09:28.996Z Has data issue: false hasContentIssue false
  • English
  • Français

Optimum concentrations of essential components for the development of Angiostrongylus costaricensis in vitro

Published online by Cambridge University Press:  05 June 2009

H. Hata
H. Hata
Affiliation:
Department of Parasitology, Chiba University School of Medicine, Inohana, Chiba City, 260, Japan
Get access Rights & Permissions [Opens in a new window]

Abstract

Third-stage larvae of Angiostrongylus costaricensis were cultured to young adult stages in Waymouth's chemically defined medium MB 752/l, which comprises higher concentrations of the essential components histidine, lysine, methionine, tryptophan, choline chloride and glucose than various other chemically defined media. The present study has shown that choline chloride and tryptophan are required at relatively higher concentrations for worm development than those of the other essential components.

Type
Research Note
Information
Journal of Helminthology , Volume 70 , Issue 2 , June 1996 , pp. 173 - 175
Copyright
Copyright © Cambridge University Press 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Barrett, J. (1981) Biochemistry of parasitic helminths. 308 pp London and Basingstoke, Macmillan Pub.CrossRefGoogle Scholar
Guttman, H.N. (1966) First defined media for Leptomonas spp. from insects. Journal of Protozoology 13, 390–392.CrossRefGoogle ScholarPubMed
Hata, H. & Kojima, S. (1991) Angiostrongylus costaricensis: culture of third-stage larvae to young adults in a defined medium. Experimental Parasitology 73, 354–361.CrossRefGoogle Scholar
Hata, H. (1994) Essential amino acids and other essential components for the development of Angiostrongylus costaricensis from third-stage larvae to young adults. Journal of Parasitology 80, 518–520.CrossRefGoogle ScholarPubMed
Jackson, G.J. & Platzer, E.G. (1974) Nutritional biotin and purine requirements, and the folate metabolism of Neoaplectana glaseri. Journal of Parasitology 60, 453–57.CrossRefGoogle ScholarPubMed
Kawanaka, M., Hayashi, S. & Ohtomo, H. (1983) Nutritional requirements of Schistosoma japonicum eggs. Journal of Parasitology 69, 857–861.CrossRefGoogle ScholarPubMed
Thirugnanam, M. & Myers, R.F. (1974) Nutrient media for plant-parasitic nematodes. VI. Nucleic acids content and nucleotide synthesis in Aphelenchoides rutgersi. Experimental Parasitology 36, 202–209.CrossRefGoogle ScholarPubMed
Trager, W. (1957) Nutrition of a hemoflagellate (Leishmania tarentolae) having an interchangeable requirement for choline or pyridoxal. Journal of Protozoology 4, 269–276.CrossRefGoogle Scholar
Waymouth, C. (1959) Rapid proliferation of sublines of NCTC clone 929 (strain L) mouse cells in a simple chemically defined medium (MB 752/I). Journal of the National Cancer Institute 22, 1003–1017.CrossRefGoogle Scholar