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The occurrence of the filarial nematode Dirofilaria repens in canine hosts from Maio Island, Cape Verde

Published online by Cambridge University Press:  01 February 2016

R. Marcos ,
C. Pereira ,
J.P. Maia ,
M. Santos ,
C. Luzzago ,
S. Lauzi ,
C. Genchi ,
A. Faustino  and
P. Puente-Payo
R. Marcos*
Affiliation:
Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal
C. Pereira
Affiliation:
Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal
J.P. Maia
Affiliation:
CIBIO Research Centre in Biodiversity and Genetic Resources, InBIO, University of Porto, Vila do Conde, Portugal Biology Department, Faculty of Sciences, University of Porto, Porto, Portugal
M. Santos
Affiliation:
Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal
C. Luzzago
Affiliation:
Department of Veterinary Science and Public Health, University of Milan, Milan, Italy
S. Lauzi
Affiliation:
Department of Veterinary Science and Public Health, University of Milan, Milan, Italy
C. Genchi
Affiliation:
Department of Veterinary Science and Public Health, University of Milan, Milan, Italy
A. Faustino
Affiliation:
Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal
P. Puente-Payo
Affiliation:
Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal
*
*E-mail: rmarcos@icbas.up.pt
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Abstract

The prevalence of canine Dirofilaria infection in Maio Island (Cape Verde) was analysed by serology, morphological and molecular identification of the parasite species. Blood and sera were collected from 150 dogs and 80 cats aged over 6 months from various localities of the island. DNA was extracted from blood and samples were screened by polymerase chain reaction (PCR) using microfilaria-specific primers. No Dirofilaria immitis was found in dogs while D. repens microfilariae were found in 5.3% of dogs and 6% were positive by PCR. The species identity was confirmed by sequencing of PCR products, which showed almost 100% homology with D. repens European sequences published in GenBank. No difference in Dirofilaria infection was observed between males and females or in dogs with different weights. However, older dogs and those from the western part of Maio Island were more frequently infected. No Dirofilaria was found in cats. This study represents the first evidence of D. repens in Cape Verde (West Africa) and highlights the need for implementing control measures and for a better surveillance of dirofilariosis in Africa.

Type
Short Communications
Information
Journal of Helminthology , Volume 91 , Issue 1 , January 2017 , pp. 87 - 90
Copyright
Copyright © Cambridge University Press 2016 

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