Hostname: page-component-8448b6f56d-t5pn6 Total loading time: 0 Render date: 2024-04-23T11:40:58.270Z Has data issue: false hasContentIssue false
  • English
  • Français

Morphology and molecular analysis of Oncicola venezuelensis (Acanthocephala: Oligacanthorhynchidae) from the ocelot Leopardus pardalis in Brazil

Published online by Cambridge University Press:  27 September 2016

E.G.N. Santos ,
M. Chame ,
V.A. Chagas-Moutinho  and
C.P. Santos
E.G.N. Santos
Affiliation:
Laboratório de Avaliação e Promoção da Saúde Ambiental, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil, 4365 – Manguinhos, Rio de Janeiro – RJ, 21040–360 Curso de Pós-graduação em Ciências Veterinárias, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, 23890–000, Seropédica, Brasil
M. Chame
Affiliation:
Laboratório de Ecologia, Escola Nacional de Saúde Pública Sergio Arouca, FIOCRUZ
V.A. Chagas-Moutinho
Affiliation:
Laboratório de Avaliação e Promoção da Saúde Ambiental, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil, 4365 – Manguinhos, Rio de Janeiro – RJ, 21040–360
C.P. Santos*
Affiliation:
Laboratório de Avaliação e Promoção da Saúde Ambiental, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil, 4365 – Manguinhos, Rio de Janeiro – RJ, 21040–360
*
*E-mail: cpsantos@ioc.fiocruz.br
Get access Rights & Permissions [Opens in a new window]

Abstract

Oncicola venezuelensis Marteau, 1977 was found parasitizing adults of Leopardus pardalis (Linnaeus) found dead in Serra da Capivara National Park, Piauí state, Brazil, a new geographical locality reported for the species. The diversity of Oncicola Travassos, 1916 species is large, but genetic data are scarce. This article presents the results of genetic, morphological and ultrastructural studies carried out for taxonomic purposes. The first ultrastructural view showed a globular, short proboscis with 36 hooks, divided into six longitudinal rows of six hooks each. Hooks differ in size and shape: hooks I, II and III have a ‘chisel-shaped’ tip. The genetic profile included new sequences of ribosomal DNA ITS1, 5.8S and ITS2, and partial 28S rRNA regions. The results of maximum-likelihood tree analyses for each region showed Oligacanthorhynchidae Southwell et Macfie, 1925 close to Gigantorhynchidae Hamann, 1892 (supported >91%). Both use mammals and birds as definitive hosts. Morphological and ultrastructural studies combined with genetic analysis shed more light on the diversity of Oncicola species.

Type
Research Papers
Information
Journal of Helminthology , Volume 91 , Issue 5 , September 2017 , pp. 605 - 612
Copyright
Copyright © Cambridge University Press 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Altschul, S.F., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J. (1990) Basic local alignment search tool. Journal of Molecular Biology 215, 403–410.CrossRefGoogle ScholarPubMed
Amin, O.M. (1987) Key to the families and subfamilies of Acanthocephala, with the erection of a new class (Polyacanthocephala) and a new order (Polyacanthorhynchida). Journal of Parasitology 73, 1216–1219.Google Scholar
Amin, O.M. (2013) Classification of the Acanthocephala. Folia Parasitologica 60, 273–305.Google Scholar
Amin, O.M., Heckmann, R.A., Mohammed, O. & Evans, R.P. (2016) Morphological and molecular descriptions of Moniliformis saudi sp. n. (Acanthocephala: Moniliformidae) from the desert hedgehog, Paraechinus aethiopicus (Ehrenberg) in Saudi Arabia, with a key to species and notes on histopathology. Folia Parasitologia 63, 014. doi: 10.14411/fp.2016.014.Google Scholar
Barger, M. (2005) A new species of Neoechinorhynchus (Acanthocephala: Neoechinorhynchidae) from turtles in Florida, U.S.A. Comparative Parasitology 72, 6–9.Google Scholar
Bolette, D.P. (2007) A new oligacanthorhynchid acanthocephalan described from the great horned owl, Bubo virginianus (Strigidae), and red-tailed hawk, Buteo jamaicensis (Accipitridae), from central Arizona, USA. Journal of Parasitology 93, 120–128.Google Scholar
Chen, M.X., Zhang, L.Q., Wen, C.G., Sun, J. & Gao, Q. (2010) Phylogenetic relationship of species in the genus Aspidogaster (Aspidogastridae, Aspidogastrinae) in China as inferred from its rDNA sequences. Acta Hydrolobiologica Sinica 34, 312–316.Google Scholar
Chisholm, L.A., Morgan, J.A., Adlard, R.D. & Whittington, I.D. (2001) Phylogenetic analysis of the Monocotylidae (Monogenea) inferred from 28S rDNA sequences. International Journal of Parasitology 31, 1537–1547.Google Scholar
Felsenstein, J. (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. Journal of Molecular Evolution 17, 368–376.Google Scholar
Fugassa, M.H. (2015) Checklist of helminths found in Patagonian wild mammals. Zootaxa 4012, 271–328.CrossRefGoogle ScholarPubMed
Fuller, C.A. & Nickol, B.B. (2011) A description of mature Oncicola venezuelensis (Acanthocephala: Oligacanthorhynchidae) from a feral house cat in the US Virgin Islands. Journal of Parasitology 97, 1099–1100.Google Scholar
García-Varela, M., de León, G.P.P., Aznar, F.J. & Nadler, S.A. (2013) Phylogenetic relationship among genera of Polymorphidae (Acanthocephala), inferred from nuclear and mitochondrial gene sequences. Molecular Phylogenetics and Evolution 68, 176–184.Google Scholar
Marteau, M. (1977) Oncicola venezuelensis n. sp. (Archiacanthocephala: Oligacanthorhynchidae) parasite de l'ocelot (Felis pardalis L.). Annales de Parasitologie 52, 25–33.Google Scholar
Murray, J.L. & Gardner, G.L. (1997) Leopardus pardalis. Mammalian Species 548, 1–10.Google Scholar
Nickol, B.B., Fuller, C.A. & Rock, P. (2006) Cystacanths of Oncicola venezuelensis (Acanthocephala: Oligacanthorhynchidae) in Caribbean termites and various paratenic hosts in the U.S. Virgin Islands. Journal of Parasitology 92, 539–542.Google Scholar
Oliveira, T.G. (1994) Neotropical cats: ecology and conservation. Sao Luís, Editora da Universidade Federal do Maranhão, EDUFMA.Google Scholar
Oliveira, T.G., Almeida, L.B. & Campos, C.B. (2013) Avaliação do risco de extinção da jaguatirica Leopardus pardalis (Linnaeus, 1758) no Brasil. Biodiversidade Brasileira 3, 66–75.Google Scholar
Posada, D. (2008) jModelTest: Phylogenetic model averaging. Molecular Biology and Evolution 25, 1253–1256.Google Scholar
Schlötterer, C., Hauser, M.T., von Haeseler, A. & Tautz, D. (1994) Comparative evolutionary analysis of rDNA ITS regions in Drosophila . Molecular Biology and Evolution 11, 513–522.Google Scholar
Sianto, L., Souza, M.V., Chame, M., Luz, M.F., Guidon, N., Pessis, A.M. & Araujo, A. (2014) Helminths in feline coprolites up to 9,000 years in Brazilian Northeast. Parasitology International 63, 851–857.CrossRefGoogle Scholar
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28, 2731–2739.Google Scholar
Travassos, L. (1917) Contribuições para o conhecimento da fauna helmintológica brasileira. Memórias do Instituto Oswaldo Cruz 9, 5–62.CrossRefGoogle Scholar
Vieira, F.M., Luque, J.L. & Muniz-Pereira, L.C. (2008) Checklist of helminth parasites in wild carnivore mammals from Brazil. Zootaxa 1721, 1–23.Google Scholar