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Isozymic pattern of lactate dehydrogenase in cases of bancroftian filariasis

Published online by Cambridge University Press:  05 June 2009

S. Misra ,
T. M. Mahopatra  and
S. Rathaur
S. Misra
Affiliation:
Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India
T. M. Mahopatra
Affiliation:
Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India
S. Rathaur
Affiliation:
Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India
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Abstract

Isozymic patterns of lactate dehydrogenase (E.C. 1.1.1.27) by polyacrylamide gel electrophoresis (PAGE) were observed m various categories of filariasis and controls, i.e. asymptomatic microfilaraemia and symptomatic amicrofilaraemia, endemic normal and non-endemic normal. Lactate dehydrogenase (LDH) activity was also observed amongst the above categories of patients. An increase in enzyme activity and change in the isozymic pattern was observed in the above categories of filaria infected serum. LDH activity doubled in asymptomatic microfilaraemia whereas in symptomatic amicrofilaraemia the increase in LDH activity was thirtyfold. The isozymic pattern of microfilaraemia cases showed the presence of three bands B4, A1B3, A2 B2, which are quite thick as compared to normal healthy subjects, whereas the patients with symptomatic amicrofilaraemia showed marked elevation of serum LDH-4 or A3B1. The LDH was partially purified by combined treatment of (NH4)2SO4 fractionation and gel filtration. The isozymic pattern of purified LDH showed a similar pattern.

Keywords

Wuchereria bancroftilactate dehydrogenasebiochemistryisoenzymesNematodafilariasis
Type
Research Article
Information
Journal of Helminthology , Volume 66 , Issue 2 , June 1992 , pp. 142 - 146
Copyright
Copyright © Cambridge University Press 1992

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