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Isoenzymatic pattern of glucose 6-phosphate dehydrogenase from Ascaris suum

Published online by Cambridge University Press:  05 June 2009

C. Cutillas ,
B. Rodriguez ,
P. German  and
D. Guevara
C. Cutillas
Affiliation:
Departamento de Microbiologa y Parasitologia, Facultad de Farmacia, Unnersidad de Sevilla, Prof, Garcia González sln, 41012 Sevilla, Spain
B. Rodriguez
Affiliation:
Departamento de Microbiologa y Parasitologia, Facultad de Farmacia, Unnersidad de Sevilla, Prof, Garcia González sln, 41012 Sevilla, Spain
P. German
Affiliation:
Departamento de Microbiologa y Parasitologia, Facultad de Farmacia, Unnersidad de Sevilla, Prof, Garcia González sln, 41012 Sevilla, Spain
D. Guevara
Affiliation:
Departamento de Microbiologa y Parasitologia, Facultad de Farmacia, Unnersidad de Sevilla, Prof, Garcia González sln, 41012 Sevilla, Spain
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Abstract

The isoenzymatic pattern of glucose 6-phosphate dehydrogenase (abbreviation G6PD) from Ascaris suum has been studied by vertical polyacrylamide gel (PAGE) and horizontal starch gel electrophoresis. After polyacrylamide gel electrophoresis, two stained zones could be identified. One corresponded to tetrazolium oxidase activity; since this zone was stained even in the absence of glucose 6-phosphate (G6P), non-specific staining could be detected. In the other zone of activity, seven regularly-spaced bands were identified by staining in the presence of G6P and NADP as substrates. By using starch gel electrophoresis, different electrophoretic patterns for G6PD have been observed in the muscular sac, intestine and reproductive system from A. suum. The existence of three different alleles of G6PD in the same individual suggests the existence of at least two genes for this enzyme.

Keywords

Ascaris suumisoenzymatic patternPAGEstarch gel electrophoresisglucose 6-phosphate dehydrogenaseNematoda
Type
Research Papers
Information
Journal of Helminthology , Volume 67 , Issue 3 , September 1993 , pp. 226 - 232
Copyright
Copyright © Cambridge University Press 1993

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