Hostname: page-component-848d4c4894-4rdrl Total loading time: 0 Render date: 2024-06-19T12:25:55.506Z Has data issue: false hasContentIssue false
  • English
  • Français

Induction of protective immunity to Brugia pahangi in jirds by drug-abbreviated infection

Published online by Cambridge University Press:  05 June 2009

Y. Horii ,
H. Nakanishi ,
A. Mori ,
M. Ueda ,
K. Kurokawa ,
M. Zaitsu ,
T. Oda  and
K. Fujita
Y. Horii
Affiliation:
Department of Medical Zoology, Nagasaki University School of Medicine, Sakamoto-machi, Nagasaki 852, Japan
H. Nakanishi
Affiliation:
Department of Medical Zoology, Nagasaki University School of Medicine, Sakamoto-machi, Nagasaki 852, Japan
A. Mori
Affiliation:
Department of Medical Zoology, Nagasaki University School of Medicine, Sakamoto-machi, Nagasaki 852, Japan
M. Ueda
Affiliation:
Department of Medical Zoology, Nagasaki University School of Medicine, Sakamoto-machi, Nagasaki 852, Japan
K. Kurokawa
Affiliation:
Department of Medical Zoology, Nagasaki University School of Medicine, Sakamoto-machi, Nagasaki 852, Japan
M. Zaitsu
Affiliation:
Department of Medical Zoology, Nagasaki University School of Medicine, Sakamoto-machi, Nagasaki 852, Japan
T. Oda
Affiliation:
Department of General Education, School of Allied Medical Sciences, Nagasaki University, Sakamoto-machi, Nagasaki 852, Japan
K. Fujita
Affiliation:
Department of Medical Zoology, Tokyo Medical and Dental University, Yushima, Bunkyo-ku 113, Japan
Get access Rights & Permissions [Opens in a new window]

Abstract

Protective immunity of homologous challenge infection was examined in jirds after drug-abbreviated infection with Brugia pahangi. Mebendazole (MBZ) treatment at the early prepatent (5–7 weeks of post infection) or the late prepatent (7–9 weeks of post infection) period was highly effective in causing almost complete eradication of the primary infection. After challenge infection, the worm burden was significantly reduced 19% (31·1 in average) and 77% (9·5) to that of the controls (38·8 and 41·7), respectively. The magnitude of eosinophil response paralleled the degree of protection. No or only a few microfilariae were seen after challenge infection in jirds treated during the prepatent periods. They were also resistant to intravenous challenge with the microfilariae of B. pahangi. MBZ treatment at the patent period was, on the contrary, incomplete against primarily infected adult worms, and was not able to induce either significant protection (30·1 vs 33·1 in control) or eosinophil response to the challenge infection.

Keywords

Brugia pahangiprotectiondrug-abbreviated infectioneosinophilNematodaimmunitymebendazoleMeriones unguiculatus
Type
Research Article
Information
Journal of Helminthology , Volume 66 , Issue 2 , June 1992 , pp. 147 - 154
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Abraham, D., Grieve, R. B., Holy, J. M. & Christensen, B. M. (1989) Immunity to larval Brugia malayi in BALB/c mice: protective immunity and inhibition of larval development. American Journal of Tropical Medicine and Hygiene, 40, 598–604.CrossRefGoogle ScholarPubMed
Blair, L. S. & Campbell, W. C. (1981) Immunization of ferrets against Dirofilaria immitis by means of chemically abbreviated infections. Parasite Immunology, 3, 143–147.CrossRefGoogle ScholarPubMed
Carlow, C. K. S. & Philipp, M. (1987) Protective immunity to Brugia malayi larvae in BALB/c mice: potential of this model for the identification of protective antigens. American Journal of Tropical Medicine and Hygiene, 37, 597–604.Google Scholar
Chandrashekar, R., Rao, U. R. & Subrahmanyam, D. (1985) Serum dependent cell-mediated immune reactions to Brugia pahangi infective larvae. Parasite Immunology, 7, 633–641.CrossRefGoogle ScholarPubMed
Chusattayanond, W. & Denham, D. A. (1984) Induction of host resistance to Brugia pahangi in jirds (Meriones unguiculatus) protected by chemoprophylaxis. Journal of Helminthology, 58, 245–249.CrossRefGoogle ScholarPubMed
Chusattayanond, W. & Denham, D. A. (1986) Attempted vaccination of jirds (Meriones unguiculatus) against Brugia pahangi with radiation attenuated infective larvae. Journal of Helminthology, 60, 149–155.Google Scholar
Denham, D. A., McGreevy, P. B., Suswillo, R. R. & Rogers, R. (1983) The resistance to re-infection of cats repeatedly inoculated with infective larvae of Brugia pahangi. Parasitology, 86, 11–18.CrossRefGoogle ScholarPubMed
Denham, D. A., Suswillo, R. R. & Chusattayanond, W. (1984) Parasitological observations on Meriones unguiculatus singly or multiply infected with Brugia pahangi. Parasitology, 88, 295–301.CrossRefGoogle ScholarPubMed
Grieve, R. B., Abraham, D., Mika-Grieve, M. & Seibert, B. P. (1988) Induction of protective immunity in dogs to infection with Dirofilaria immitis using chemically-abbreviated infections. American Journal of Tropical Medicine and Hygiene, 39, 373–379.CrossRefGoogle ScholarPubMed
Hayashi, Y., Nakagaki, K., Nogami, S., Hammerberg, B. & Tanaka, H. (1989) Protective immunity against Brugia malayi infective larvae in mice. I. Parameters of active and passive immunity. American Journal of Tropical Medicine and Hygiene, 41, 650–656.Google Scholar
Horii, Y., Nakanishi, H., Mori, A., Zaitsu, M., Ueda, M., Kurokawa, K., Oda, T. & Fujita, K. (1989) Eosinophil hyporesponse of jirds induced by microfilariae of Brugia pahangi. American Journal of Tropical Medicine and Hygiene, 41, 183–188.CrossRefGoogle ScholarPubMed
Johnson, P., Mackenzie, C. D., Suswillo, R. R. & Denham, D. A. (1981) Serum-mediated adherence of feline granulocytes to microfilariae of Brugia pahangi in vitro: variations with parasite maturation. Parasite Immunology, 3, 69–80.CrossRefGoogle ScholarPubMed
Klei, T. R., McCall, J. W. & Malone, J. B. (1980) Evidence for increased susceptibility of Brugia pahangi-infected jirds (Meriones unguiculatus) to subsequent homologous infections. Journal of Helminthology, 54, 161–165.CrossRefGoogle ScholarPubMed
Klei, T. R., Enright, F. M., McDonough, K. C. & Coleman, S. U. (1988) Brugia pahangi: granulomatous lesion development in jirds following single and multiple infections. Experimental Parasitology, 66, 132–139.CrossRefGoogle ScholarPubMed
Lammie, P. J. & Katz, S. P. (1983a) Immunoregulation in experimental filariasis. I. In vitro suppression of mitogen-induced blastogenesis by adherent cells from jirds chronically infected with Brugia pahangi. Journal of Immunology, 130, 1381–1385.Google Scholar
Lammie, P. J. & Katz, S. P. (1983b) Immunoregulation in experimental filariasis. II. Responses to parasite and nonparasite antigens in jirds with Brugia pahangi. Journal of Immunology, 130, 1386–1389.Google Scholar
Lammie, P. J. & Katz, S. P. (1984) Immunoregulation in experimental filariasis. III. Demonstration and characterization of antigen-specific suppressor cells in the spleen of Brugia pahangi-infected jirds. Immunology, 52, 211–219.Google Scholar
Leiva, L. E. & Lammie, P. J. (1989) Regulation of parasite antigen-induced T cell growth factor activity and proliferative responsiveness in Brugia pahangi-infected jirds. Journal of Immunology, 142, 1304–1309.CrossRefGoogle ScholarPubMed
Mackenzie, C. D. (1980) Eosinophil leukocytes in filarial infections. Transactions of the Royal Society of Tropical Medicine and Hygiene, 74 (Suppl). 51–58.CrossRefGoogle Scholar
Nakanishi, H., Horii, Y., Terashima, K. & Fujita, K. (1989) Mechanisms of acquired immunity against Brugia pahangi: inflammatory cell responses and antibody formation following the challenge infection in mice immunized with naive infective larvae. Tropical Medicine, 31, 25–34.Google Scholar
Nutman, T. B., Kumaraswami, V. & Ottesen, E. A. (1987) Parasite-specific anergy in human filariasis: insights after analysis of parasite antigen-driven lymphokine production. Journal of Clinical Investigation, 79, 1516–1523.CrossRefGoogle ScholarPubMed
Piessens, W. F., Partono, F., Hoffman, S. L., Ratiwayanto, S., Piessens, P. W., Palmieri, J. R., Koiman, I., Dennis, D. T. & Carney, W. P. (1982) Antigen-specific suppressor T lymphocytes in human lymphatic filariasis. New England Journal of Medicine, 307, 144–148.CrossRefGoogle ScholarPubMed
Wadee, A. A., Vickery, A. C. & Piessens, W. F. (1987) Characterization of immunosuppressive proteins of Brugia malayi microfilariae. Acta Tropica, 44, 343–352.Google ScholarPubMed
Yamaguchi, Y., Suda, T., Suda, J., Eguchi, M., Miura, Y., Harada, N., Tominaga, A. & Takatsu, K. (1988) Purified interleukin 5 supports the terminal differentiation and proliferation of murine eosinophilic precursors. Journal of Experimental Medicine, 167, 43–56.Google Scholar
Yates, J. A. & Higashi, G. I. (1985) Brugia malayi: vaccination of jirds with 60cohalt-attenuated infected stage larvae protects against homologous challenge. American Journal of Tropical Medicine and Hygiene, 34, 1132–1137.CrossRefGoogle ScholarPubMed
Yates, J. A. & Higashi, G. 1. (1986) Ultrastructural observations on the fate of Brugia malayi in jirds previously vaccinated with irradiated infective stage larvae. American Journal of Tropical Medicine and Hygiene, 35, 982–987.Google Scholar