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In vitro screening for cestocidal activity of three species of Cassia plants against the tapeworm Raillietina tetragona

Published online by Cambridge University Press:  20 March 2012

S. Kundu  and
L.M. Lyndem
S. Kundu
Affiliation:
Department of Zoology, Visva Bharati University, Santiniketan 731235, West Bengal, India
L.M. Lyndem*
Affiliation:
Department of Zoology, Visva Bharati University, Santiniketan 731235, West Bengal, India
*
* E-mail: lyndemlarisha@hotmail.com
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Abstract

Different species of Cassia plant are widely available in India and are commonly used either for their laxative, antimicrobial or antibacterial activity. In the present study the effectiveness in vitro of the crude alcoholic extracts of three species, namely Cassia alata, C. occidentalis and C. angustifolia, in the early paralysis and mortality of the fowl tapeworm Raillietina tetragona at concentrations ranging from 5 to 80 mg/ml was investigated. Time of paralysis and death were monitored frequently. Immediately after paralysis the tapeworms were processed for electron microscopic studies. While the untreated or control parasites survived for 81.93 ± 5.85 h, the parasites treated with C. alata took less time (1.68 ± 0.27 h) to be paralysed, followed by those treated with C. angustifolia (2.95 ± 0.29 h). Although C. occidentalis took more time (4.13 ± 0.31 h) to paralyse, in combination with either C. alata or C. angustifolia the time taken to paralyse became shorter. All the plant-treated parasites showed irrevocable changes in the scolex and proglottids as compared with the control, and these observations are comparable with those obtained with praziquantel. These results indicate that the three plants tested can be claimed to have anthelmintic activity in addition to their known properties, both when used individually and in combination. Further investigations will be required to evaluate their mechanism of action.

Type
Research Papers
Information
Journal of Helminthology , Volume 87 , Issue 2 , June 2013 , pp. 154 - 159
Copyright
Copyright © Cambridge University Press 2012 

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