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In vitro culture of tetrathyridia of Mesocestoides corti using a gel based diphasic medium

Published online by Cambridge University Press:  12 April 2024

J. Chernin ,
D. Thompson  and
D. Swaine
J. Chernin*
Affiliation:
School of Biological Sciences, University of Portsmouth, King Henry Building, Portsmouth, PO1 2DY, Hants, UK
D. Thompson
Affiliation:
Research and Development, Oxoid Ltd, Wade Road, Basingstoke, RG24 8PW, Hants, UK
D. Swaine
Affiliation:
Research and Development, Oxoid Ltd, Wade Road, Basingstoke, RG24 8PW, Hants, UK
*
*Fax: 0293 92 842070 Email: jack.chernin@port.ac.uk
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Abstract

Tetrathyridia of Mesocestoides corti were cultured in vitro in a diphasic medium consisting of a liquid medium (CMRL Sigma) and a thixotropic nutrient gel (Oxoid). Tests demonstrated that a 50% medium/gel mixture produced optimum conditions for the survival and development of tetrathyridia. Established anthelminthic drugs were inoculated into the gel which demonstrated that this system can be used for preliminary anthelminthic drug screening. The development and survival of the tetrathyridia were influenced by the addition of pepsin, trypsin and liver peptone to the culture media. The development and maturation of proglottids were observed in addition to asexual reproduction by the process of budding. Tetrathyridia maintained in vitro and reinfected into both mouse and rat hosts retained their viability.

Type
Review Article
Information
Journal of Helminthology , Volume 76 , Issue 1 , March 2002 , pp. 21 - 25
Copyright
Copyright © Cambridge University Press 2002

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References

Barrett, N.J., Smyth, J.D. & Ong, SJ. (1982) Spontaneous sexual differentiation of Mesocestoides cortiin vitro. International Journal for Parasitology 12, 315–322.CrossRefGoogle ScholarPubMed
Ong, SJ. & Smyth, J.D. (1985) Effects of some culture factors on sexual differentiation of M esocestoides corti from tetrathyridia in vitro. International Journal for Parasitology 16, 361–368.CrossRefGoogle Scholar
Smyth, J.D. (1979) Echinococcus granulosus and E. multilocularis: in vitro culture of strobilar stages from protoscoleces. Angewandte Parasitologie 20, 137–148.Google Scholar
Smyth, J.D. (1985) In vitro culture of Echinococcus spp. Proceedings of the 13th International Congress of Hydatidology, Madrid 1985, pp. 84–89 Comunidad de Madrid, Coserjia de Agricultura y Granaderia, Madrid.Google Scholar
Smyth, J.D. (1987) Asexual and sexual differentiation in cestodes: especially Mesocestoides and Echinococcus, pp. 19–33 in McInnis, A. (Ed) Molecular paradigms for eradicating parasites. New York, Alan R. Liss.Google Scholar
Specht, D. & Voge, M. (1965) Asexual multiplication of Mesocestoides corti tetrathyridia in laboratory animals. Journal of Parasitology 51, 269–272.CrossRefGoogle Scholar
Tallin, M. (1965) The biology of the tetrathyridia of Mesocestoides corti infected into laboratory rats and mice. PhD thesis, University of Portsmouth.Google Scholar
Thompson, R.C.A., Jue Sue, L.P. & Buckley, S.P. (1982) In vitro development of the strobilar stage of Mesocestoides corti . International Journal for Parasitology 12, 303–314.CrossRefGoogle ScholarPubMed
Voge, M. (1967) Development in vitro of Mesocestoides (Cestoda) from oncosphere to young tetrathyridium. Journal of Parasitology 53, 78–82.CrossRefGoogle ScholarPubMed
Voge, M. & Seigel, J.S. (1968) Continuous growth in vitro of Mesocestoides (Cestoda) from oncospheres to fully developed tetrathyridium. Journal of Parasitology 54, 269–271.CrossRefGoogle ScholarPubMed