Hostname: page-component-7479d7b7d-t6hkb Total loading time: 0 Render date: 2024-07-12T09:22:44.486Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunological Studies on Experimental Infection of Pigs with Ascaris suum Goeze, 1782. II. The Use of the Conglutinating Complement Absorption Test for the Demonstration of Ascaris Antibody*

Published online by Cambridge University Press:  05 June 2009

L. F. Taffs
L. F. Taffs
Affiliation:
Department of Animal Pathology, School of Veterinary Medicine Cambridge**
Get access Rights & Permissions [Opens in a new window]

Extract

The technique of the conglutinating complement absorption test, which has been used to detect antibody in pigs infected with Ascaris, and the method employed for rendering pig serum suitable for use in this test, have been described.

Antibodies against Ascaris can be detected by the use of the following antigens: (1) An alcoholic extract of ovaries, oviducts and uteri of the female adult Ascaris. (2) Boiled and unboiled saline extracts of whole worm. (3) Polysaccharide fraction of whole worm.

On an examination of antisera from pigs infected with Oesophagostomum dentatiim, no cross-reaction between Oesophagostomum and A. sttum was observed using a saline extract of whole Ascaris worm as antigen.

Type
Research Article
Information
Journal of Helminthology , Volume 37 , Issue 4 , December 1963 , pp. 369 - 380
Copyright
Copyright © Cambridge University Press 1963

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bordet, J. and Gay, F. P., 1906.—“Sur les relations des sensibilisatrices avec l” Ann. Inst. Pasteur, 20, 467–498.Google Scholar
Campbell, D. H., 1936.—“An antigenic polysaccharide fraction of Ascaris lumbricoides (from hog)”. J. infect. Dis., 59, 266–280.CrossRefGoogle Scholar
Ehrlich, P. and Sachs, H., 1902.—“Über den Mechanisms der Amboccptorenwirkung”. Berl. hlin. Wschr.. 39, 492–496.Google Scholar
Hole, N. H. and Coombs, R. R. A., 1947a.—“The conglutination phenomenon. I. An introduction to the conglutination phenomenon and an account of the observations and views of previous investigators.” J. Hyg. (Catnb.), 45. 480–489.Google Scholar
Hole, N. H., 1947b.—”The conglutination phenomenon. II. The technique of the conglutinating complement absorption test compared with the haemolytic complement fixation test”. J. Hyg., 45, 490–496.Google Scholar
Hole, N. H., 1947c.—”The conglutination phenomenon. III. The conglutinating complement absorption test in glanders.” J. Hyg., 45, 497–503.Google Scholar
Stoker, M. G. P., Coombs, R. R. A. and Bedson, S. P., 1950.—“The application of the conglutinating complement absorption test to virus systems”. Brit. J. exp. Path., 31, 217–232.Google ScholarPubMed
Streng, O., 1910.—“Alexinets btydelse fOr ulflockmingen af mikrober hoch blodkulor”. Finska. LākSāllsk. HandL, 52, 95–106.Google Scholar
Taffs, L. F., 1961.—“Immunological studies on experimental infection of pigs with Ascaris suum Goeze, 1782. I. An introduction with a review of the literature and the demonstration of complement-fixing antibodies in the serum.” J. Helminth., 35, 319–344.CrossRefGoogle ScholarPubMed
Wiggin, N. J. B. 1955.—“Studies on conglutination with special reference to complement, anticomplemcntary action, and immunoconglutinin.” Ph.D. Thesis, University of Cambridge, England.Google Scholar