Hostname: page-component-848d4c4894-xfwgj Total loading time: 0 Render date: 2024-06-23T07:13:28.597Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunodiagnosis of ocular toxocariasis using Western-blot for the detection of specific anti-Toxocara IgG and CAP™ for the measurement of specific anti-Toxocara IgE

Published online by Cambridge University Press:  12 April 2024

J.-F. Magnaval ,
L. Malard ,
B. Morassin  and
R. Fabre
J.-F. Magnaval*
Affiliation:
Service de Parasitologie, CHU Rangueil, 31403 Toulouse, 4, France
L. Malard
Affiliation:
Service de Parasitologie, CHU Rangueil, 31403 Toulouse, 4, France
B. Morassin
Affiliation:
Service de Parasitologie, CHU Rangueil, 31403 Toulouse, 4, France
R. Fabre
Affiliation:
Service de Parasitologie, CHU Rangueil, 31403 Toulouse, 4, France
*
*Fax: 33 5 61 14 59 72 E-mail: magnaval@cict.fr.
Get access Rights & Permissions [Opens in a new window]

Abstract

A prospective multicentric study was carried out to assess both the performance of Western-blot (WB) detecting specific anti-Toxocara IgG and that of CAP™ measuring specific IgE titre for the immunodiagnosis of ocular toxocariasis. For 14 outpatients presenting ophthalmic symptoms (choroiditis, chorioretinitis, papillar oedema, hyalitis, retinal detachment and/or uveitis), samples of serum and aqueous fluid (AF) were sent to the Department of Parasitology, University Hospitals, Toulouse, France. All patients but two tested positive with WB on the serum; 13 WB tests were performed on the AF, 12 of which were positive. The two patients who had a negative WB serum result tested positive for the AF. Specific IgE detection was considered as a complementary test of WB. Two patients showed a greater specific IgE titre in the AF than in the serum, and one had a positive result in the AF, but not in the serum. These six patients were considered as clear cases of ocular toxocariasis. Western-blot coupled with specific anti-Toxocara IgE detection appeared therefore to be an accurate procedure for the immunodiagnosis of ocular toxocariasis, provided the testing was simultaneously performed on the serum and AF.

Type
Research Article
Information
Journal of Helminthology , Volume 76 , Issue 4 , December 2002 , pp. 335 - 339
Copyright
Copyright © Cambridge University Press 2002

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Akao, N., Kondo, K., Okamoto, T. & Yoshimura, H. (1983) Antigenic analysis of excretory–secretory products of 2nd stage larvae of Toxocara canis and the antigen recognition in the course of infection. Japanese Journal of Parasitology 32, 541–548.Google Scholar
Banares, A., Jover, J.A., Fernandez-Gutierrez, B., Benitez del Castillo, J.M., Garcia, J., Vargas, E. & Hernandez-Garcia, C. (1997) Patterns of uveitis as a guide in making rheumatologic and immunologic diagnoses. Arthritis and Rheumatism 40, 358–370.CrossRefGoogle ScholarPubMed
Barriga, O.O. (1988) A critical look at the importance, prevalence and control of toxocariasis and the possibilities of immunological control. Veterinary Parasitology 29, 195–234.CrossRefGoogle Scholar
Beaver, P.C., Snyder, C.H. & Carrera, G.M. (1952) Chronic eosinophilia due to visceral larva migrans. Pediatrics 9, 7–19.CrossRefGoogle ScholarPubMed
Beerlandt, N., Dralands, L., Vanginderdeuren, R., Blanckaert, J., Vanbesien, B., Van Cauwenberghe, J., De Keersmaecker, L. & Vervoort, T. (1995) Ocular toxocariasis in a 36-year-old patient: a case report. Bulletin de la Société Belge d'Ophtalmologie 259, 177–181.Google Scholar
Benitez del Castillo, J.M., Herreros, G., Guillen, J.L., Fenoy, S., Banares, A. & Garcia, J. (1995) Bilateral ocular toxocariasis demonstrated by aqueous humor enzyme-linked immunosorbent assay. American Journal of Ophthalmology 119, 514–516.CrossRefGoogle ScholarPubMed
Bowman, D.D., Mika-Grieve, M. & Grieve, R.B. (1987) Circulating excretory antigens levels and specific antibody responses in mice infected with Toxocara canis . American Journal of Tropical Medicine and Hygiene 36, 75–82.CrossRefGoogle ScholarPubMed
Brasseur, G., Charlin, J.F., Brasseur, P. & Langlois, J. (1984) Toxocarose oculaire. Acquisitions diagnostiques et thérapeutiques. Journal Français d'Ophtalmologie 7, 221–226.Google Scholar
Courtade, H., Recco, P., Magnaval, J.-F. & Charlet, J.-P. (1995) Etude comparative de 2 tests ELISA Toxocara vis-à-vis du western-blot. Bulletin de la Société Française de Parasitologie 13, 37–53.Google Scholar
De Savigny, D.H. (1975) In vitro maintenance of Toxocara canis larvae and a simple method for the production of Toxocara ES antigens for use in serodiagnostic tests for visceral larva migrans. Journal of Parasitology 61, 781–782.CrossRefGoogle Scholar
De Savigny, D.H., Voller, A. & Woodruff, A.W. (1979) Toxocariasis: serological diagnosis by enzyme immunoassay. Journal of Clinical Pathology 32, 284–288.CrossRefGoogle ScholarPubMed
De Souza, E.C. & Nakashima, Y. (1995) Diffuse unilateral subacute neuroretinitis. Report of transvitreal surgical removal of a subretinal nematode. Ophthalmology 102, 1183–1186.CrossRefGoogle ScholarPubMed
Gass, J.D. & Braunstein, R.A. (1983) Further observations concerning the diffuse unilateral subacute neuroretinitis syndrome. Archives of Ophthalmology 101, 1689–1697.CrossRefGoogle ScholarPubMed
Genchi, C., Falagiani, P., Riva, G., Tinelli, M., Brunello, F., Boero, M. & Almaviva, M. (1988) IgE and IgG antibodies in Toxocara canis infection. A clinical evaluation. Annals of Allergy 61, 43–46.Google ScholarPubMed
Gillespie, S.H., Dinning, W.J., Voller, A. & Crowcroft, N.S. (1993) The spectrum of ocular toxocariasis. Eye 7, 415–418.CrossRefGoogle ScholarPubMed
Glickman, L.T. (1993) The epidemiology of human toxocariasis. pp. 3–10 in Lewis, J.W. & Maizels, R.M. (Eds) Toxocara and toxocariasis. London, British Society for Parasitology and Institute of Biology.Google Scholar
Glickman, L.T. & Schantz, P.M. (1981) Epidemiology and pathogenesis of zoonotic toxocariasis. Epidemiologic Reviews 3, 230–250.CrossRefGoogle ScholarPubMed
Glickman, L.T., Schantz, P.M. & Grieve, R.B. (1986) Toxocariasis. pp. 201–231 in Walls, K.W. & Schantz, P.M. (Eds) Immunodiagnosis of parasitic diseases, volume 1: helmintic diseases. New York, Academic Press.Google Scholar
Gueglio, B., de Gentile, L., Nguyen, J.M., Achard, J., Chabasse, D. & Marjolet, M. (1994) Epidemiologic approach to human toxocariasis in western France. Parasitology Research 80, 531–536.CrossRefGoogle ScholarPubMed
Maetz, H.M., Kleinstein, R.N., Federico, D. & Wayne, J. (1987) Estimated prevalence of ocular toxoplasmosis and toxocariasis in Alabama. Journal of Infectious Diseases 156, 414.CrossRefGoogle ScholarPubMed
Mafee, M.F., Goldberg, M.F., Cohen, S.B., Gotsis, E.D., Safran, M., Chekuri, L. & Raofi, B. (1989) Magnetic resonance imaging versus computed tomography of leukocoric eyes and use of in vitro proton magnetic resonance spectroscopy of retinoblastoma. Ophthalmology 96, 965–975.CrossRefGoogle ScholarPubMed
Magnaval, J.-F., Fabre, R., Maurieres, P., Charlet, J.-P. & De Larrard, B. (1991) Application of the westernblotting procedure for the immunodiagnosis of human toxocariasis. Parasitology Research 77, 697–702.CrossRefGoogle Scholar
Magnaval, J.-F., Fabre, R., Maurieres, P., Charlet, J.-P. & De Larrard, B. (1992) Evaluation of an immunoenzymatic assay detecting specific anti-Toxocara immunoglobulin E for diagnosis and posttreatment follow-up of human toxocariasis. Journal of Clinical Microbiology 30, 2269–2274.CrossRefGoogle ScholarPubMed
Meyer-Riemann, W., Petersen, J. & Vogel, M. (1999) Extraktionsversuch einer intraretinalen Nematode im papillomakularen Bundel. Klinische Monatsblatter für Augenheilkunde 214, 116–119.CrossRefGoogle Scholar
Neafie, R.C. & Connor, D.H. (1976) Visceral larva migrans. pp. 433–436 in Binford, C.H. & Connor, D.H. (Eds) Pathology of tropical and extraordinary diseases. Washington, DC, Armed Forces Institute of Pathology.Google Scholar
Petithory, J.C. & Beddock, A. (1997) Rôle de Toxocara cati dans le syndrome de larva migrans viscérale. Bulletin de la Société Française de Parasitologie 15, 199–211.Google Scholar
Templeton, P.A. & Rao, K.C. (1987) Computed tomography of Toxocara canis endophthalmitis. Journal of Computer Assisted Tomography 11, 99–101.CrossRefGoogle ScholarPubMed
Tran, V.T., Lumbroso, L., LeHoang, P. & Herbort, C.P. (1999) Ultrasound biomicroscopy in peripheral retinovitreal retinovitreal toxocariasis. American Journal of Ophthalmology 127, 607–609.CrossRefGoogle ScholarPubMed
Van Asseldeft, O.W. (1985) Reference values for the total and differential leukocyte count. Blood Cells 11, 77–96.Google Scholar
Wan, W.L., Cano, M.R., Pince, K.J. & Green, R.L. (1991) Echographic characteristics of ocular toxocariasis. Ophthalmology 98, 28–32.CrossRefGoogle ScholarPubMed
Wilder, H.C. (1950) Nematode endophthalmitis. Transactions of the American Academy of Ophthalmology and Otolaryngology 55, 99–109.Google ScholarPubMed
Yoshida, M., Shirao, Y., Asai, H., Nagase, H., Nakamura, H., Okazawa, T., Kondo, K., Takayanagi, T.H., Fujita, K. & Akao, N. (1999) A retrospective study of ocular toxocariasis in Japan: correlation with antibody prevalence and ophthalmological findings of patients with uveitis. Journal of Helminthology 73, 357–361.CrossRefGoogle ScholarPubMed