Hostname: page-component-848d4c4894-2xdlg Total loading time: 0 Render date: 2024-06-29T20:32:24.209Z Has data issue: false hasContentIssue false
  • English
  • Français

Histopathological changes during experimental infections of calves with Cooperia punctata

Published online by Cambridge University Press:  12 April 2024

R.R. Rodrigues ,
S.M. Gennari ,
J.L. Guerra ,
M.B. Contieri ,
A.L. Abdalla  and
D.M.S.S. Vitti
R.R. Rodrigues
Affiliation:
Centro de Energia Nuclear na Agricultura, Avenida Centenário 303, CEP 13.400-970, Universidade de São Paulo, Piracicaba, Brazil, :
S.M. Gennari*
Affiliation:
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Universidade de São Paulo, SP, Brazil
J.L. Guerra
Affiliation:
Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, SP, Brazil
M.B. Contieri
Affiliation:
Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, SP, Brazil
A.L. Abdalla
Affiliation:
Centro de Energia Nuclear na Agricultura, Avenida Centenário 303, CEP 13.400-970, Universidade de São Paulo, Piracicaba, Brazil, :
D.M.S.S. Vitti
Affiliation:
Centro de Energia Nuclear na Agricultura, Avenida Centenário 303, CEP 13.400-970, Universidade de São Paulo, Piracicaba, Brazil, :
*
*Author for correspondence Fax: 11 3091 7928 Email: sgennari@usp.br
Get access Rights & Permissions [Opens in a new window]

Abstract

Eleven male two-month-old Holstein calves were used to determine the pathological changes induced by a Cooperia punctata infection. After weaning, ten calves received a single oral dose of 45,000 C. punctata infective larvae. One calf remained as a non-infected control. Groups of two calves were killed on days 7, 14, 21, 28 and 35 post-infection (p.i.) for determination of worm burdens and histopathological evaluation. The small intestine was sub-divided into three sections of approximately equal length, and representative samples of mucosa were fixed in 10% formalin, cut, and stained with haematoxylin-eosin. Samples of intestinal contents and mucosal digests were taken and fixed in 10% formalin for an estimation of total worm burdens. An increase in the number of adult parasites and a decrease in the number of larvae were observed with time (P<0.001). A higher concentration of worms was found in the first segment of the small intestine during the five weeks of observation. Histology showed larvae in the intestinal mucosa on day 7 p.i., with a discrete increase in the cellular response. Adult worms and a marked cellular infiltrate with eosinophils and neutrophils were present on day 21 p.i., and these persisted until day 35 p.i. Microcysts resulting from worm destruction were observed from day 21 p.i.

Type
Review Article
Information
Journal of Helminthology , Volume 78 , Issue 2 , June 2004 , pp. 167 - 171
Copyright
Copyright © Cambridge University Press 2004

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alicata, J.E. Lynd, F.T. (1961) Growth rate and other signs of infection in calves experimentally infected with Cooperia punctata . American Journal of Veterinary Research 22: 704–707.Google ScholarPubMed
Armour, J., Bairden, K., Holmes, P.H., Parkins, J.J., Ploeger, H., Salman, S.K. Mcwilliam, P.N. (1987) Pathophysiological and parasitological studies on Cooperia oncophora infections in calves. Research in Veterinary Science 42: 373–381.CrossRefGoogle ScholarPubMed
Bailey, W.S. (1949) Studies on calves experimentally infected with Cooperia punctata . American Journal of Veterinary Research 10: 119–129.Google ScholarPubMed
Balic, A., Bowles, V.M. Meeusen, E.N.T. (2000) The immunobiology of gastrointestinal nematode infections in ruminants. Advances in Parasitology 45: 182–249.Google ScholarPubMed
Bianchin, I., Honer, M.R. Nascimento, Y.A. (1990) The epidemiology of helminths in Nellore beef cattle in the cerrados of Brazil. in Epidemiology of bovine nematode parasites in the Americas. 16th Word Buiatrics Congress and 6th Latin American Buiatrics Congress, Salvador, Bahia, Brazil, 1990.Google Scholar
Cheema, K.J. Scofield, A.M. (1985) The influence of level of infection of rats with Nippostrongylus brasiliensis on the haematology and phospholipase activity and mast cell numbers in the small intestine and colon. International Journal for Parasitology 15: 55–60.CrossRefGoogle ScholarPubMed
Claerebout, E. Vercruysse, J. (2000) The immune response and the evaluation of acquired immunity against gastrointestinal nematodes in cattle: a review. Parasitology 120, S25–S42.CrossRefGoogle ScholarPubMed
Coop, R.L., Sykes, A.R. Angus, K.W. (1979) The pathogenicity of daily intakes of Cooperia oncophora larvae in growing calves. Veterinary Parasitology 5: 261–269.CrossRefGoogle Scholar
Dawkins, H.J.S., Windon, R.G. Eagleson, G.K. (1989) Eosinophil responses in sheep selected for high and low responsiveness to Trichostrongylus colubriformis . International Journal for Parasitology 19: 199–205.CrossRefGoogle ScholarPubMed
Garside, P., Kennedy, M.W., Wakelin, D. Lawrence, C. (2000) Immunopathology of intestinal helminth infection. Parasite Immunology 22: 605–612.CrossRefGoogle ScholarPubMed
Honer, M.R. Vieira-Bressan, M.C.R. (1992) Nematódeos de bovinos no Brasil–O estado da pesquisa. Revista Brasileira de Parasitologia Veterinária 1: 67–79.Google Scholar
Hung, S.L. (1926) The pathology of Cooperia punctata infestation in calves. North American Veterinarian 7: 33–36.Google Scholar
Huntley, J.F., Newlands, G.F.J. Miller, H.R.P. (1984) The isolation and characterization of globule leucocytes: their derivation from mucosal mast cells in parasitized sheep. Parasite Immunology 6: 371–390.CrossRefGoogle ScholarPubMed
Huntley, J.F., Patterson, M., Mackellar, A., Jackson, F., Stevenson, L.M. Coop, R.L. (1995) A comparison of mast cell and eosinophil responses of sheep and goats to gastrointestinal nematode infections. Research in Veterinary Science 58: 5–10.CrossRefGoogle ScholarPubMed
Jones, D.G. (1993) The eosinophil. Journal of Comparative Pathology 108: 317–335.CrossRefGoogle ScholarPubMed
Leland, S.E. (1995) Monospecific nematode infections of donor calves with Cooperia punctata . Veterinary Parasitology 60: 111–118.CrossRefGoogle ScholarPubMed
Lima, W.S. (1998) Seasonal infection pattern of gastrointestinal nematodes of beef cattle in Minas Gerais State, Brazil. Veterinary Parasitology 74: 203–214.CrossRefGoogle Scholar
Mackenzie, C.D., Jungery, M., Taylor, P.M. Ogilvie, B.M. (1981) The in vitro interaction of eosinophils, neutrophils, macrophages and mast cells with nematode surfaces in the presence of complement and antibodies. Journal of Pathology 133: 161–175.CrossRefGoogle Scholar
Miller, H.R.P. (1984) The protective mucosal response against gastrointestinal nematodes in ruminant and laboratory animals. Veterinary Immunology and Immunopathology 6: 167–259.CrossRefGoogle ScholarPubMed
Oliveira, R.O. (2001) Estudo da inter-relação parasito-hospedeiro em bovinos infectados com Cooperia punctata. Efeito da suplementação protéica sobre as alterações fisiopatológicas em bezerros com infecção única. 127 pp. Master's thesis, ICB (Instituto de Ciências Biomédicas), Universidade de São Paulo.Google Scholar
Ransom, B.H. (1920) Intestinal lesions in calves due to Cooperia punctata. Journal of Parasitology 7, 96.Google Scholar
Rothwell, T.L.W. (1989) Immune expulsion of parasitic nematodes from the alimentary tract. International Journal for Parasitology 19: 139–168.CrossRefGoogle ScholarPubMed
Rothwell, T.L.W., Windon, R.G. Horsburgh, B.A. Anderson, B.H. (1993) Relationship between eosinophilia and responsiveness to infection with Trichostrongylus colubriformis in sheep. International Journal for Parasitology 23: 203–211.CrossRefGoogle ScholarPubMed
Satrija, F. Nansen, P. (1992) Experimental infections with Cooperia oncophora in calves. Acta Veterinaria Scandinavica 33: 229–236.CrossRefGoogle ScholarPubMed
Schuberth, H.J., Freigofas, R., Daugschies, A. Leibold, W. (2000) Assessment of antibody-independent cellular cytotoxicity (AICC) of porcine neutrophilic granulocytes by quantitative flow cytometry. Lack of modulation by larval products of Oesophagostomum dentatum . Journal of Veterinary Medicine 47: 607–617.CrossRefGoogle ScholarPubMed
Wassom, D.L. Gleich, G.J. (1979) Damage to Trichinella spiralis newborn larvae by eosinophil major basic protein. American Journal of Tropical Medicine and Hygiene 28: 860–863.CrossRefGoogle ScholarPubMed
Winter, M.D., Wright, C. Lee, D.L. (1997) The mast cell and eosinophil response of young lambs to a primary infection with Nematodirus battus . Parasitology 114: 189–193.CrossRefGoogle ScholarPubMed
Vieira-Bressan, M.C.R., Gennari, S.M., Santos Filho, J.P. Rogero, J.R. (1995) Pathophysiological observations on calves concurrently infected with Cooperia punctata and Haemonchus placei . Arquivo Brasileiro de Medicina Veterinária e Zootecnia 47: 53–54.Google Scholar